PMID- 29687250
OWN - NLM
STAT- MEDLINE
DCOM- 20180814
LR  - 20191210
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 410
IP  - 18
DP  - 2018 Jul
TI  - Comprehensive monitoring of specific metabolites of tri-(2-ethylhexyl) 
      trimellitate (TEHTM) in urine by column-switching liquid chromatography-tandem 
      mass spectrometry.
PG  - 4343-4357
LID - 10.1007/s00216-018-1086-7 [doi]
AB  - Tri-(2-ethylhexyl) trimellitate (TOTM or TEHTM) is a substitute for the 
      plasticizer di-(2-ethylhexyl) phthalate (DEHP). Here, a fast and robust HPLC 
      method is presented for the first time enabling the simultaneous quantification 
      of several TEHTM metabolites in urine. These are the three TEHTM monoester 
      isomers 1-mono-(2-ethylhexyl) trimellitate (1-MEHTM), 2-mono-(2-ethylhexyl) 
      trimellitate (2-MEHTM), and 4-mono-(2-ethylhexyl) trimellitate (4-MEHTM) as well 
      as several selected side chain oxidized monoesters of TEHTM, namely, 
      1-mono-(2-ethyl-5-hydroxyhexyl) trimellitate (5OH-1-MEHTM), 
      2-mono-(2-ethyl-5-hydroxyhexyl) trimellitate (5OH-2-MEHTM), 
      1-mono-(2-ethyl-5-oxohexyl) trimellitate (5oxo-1-MEHTM), 
      2-mono-(2-ethyl-5-oxohexyl) trimellitate (5oxo-2-MEHTM), 
      1-mono-(2-ethyl-5-carboxypentyl) trimellitate (5cx-1-MEPTM), 
      2-mono-(2-ethyl-5-carboxypentyl) trimellitate (5cx-2-MEPTM), 
      2-mono-(2-carboxymethylhexyl) trimellitate (2cx-2-MMHTM), and 
      1-mono-(2-carboxymethylhexyl) trimellitate (2cx-1-MMHTM). The method is 
      characterized by a short sample preparation, for which the urine samples are 
      enzymatically hydrolyzed and cleaned up by an online column arrangement. 
      Separation of the analytes is enabled using liquid chromatography coupled with 
      tandem mass spectrometry. Thus, in less than 30 min, 11 postulated metabolites of 
      TEHTM can be selectively and sensitively quantified. The method is distinguished 
      by its wide linear working range of up to 1800 mug/L with detection limits ranging 
      from 0.3 mug/L (for 5oxo-1-MEHTM) to 1.5 mug/L (for 1-MEHTM). Precision and 
      repeatability of the method were proven with determined relative standard 
      deviations between 2.5 and 11.3%. The selection of the analytes of this method 
      was based on a pilot study, by which several potential TEHTM metabolites were 
      investigated in human urine of an orally exposed volunteer. Thus, the here 
      presented method is a perfect tool for human biomonitoring of TEHTM exposure. 
      Graphical abstract Analysis of several postulated TEHTM metabolites in human 
      urine using LC-MS/MS.
FAU - Hollerer, Christine
AU  - Hollerer C
AD  - Institute and Outpatient Clinic of Occupational, Social and Environmental 
      Medicine, Friedrich-Alexander-Universitat Erlangen-Nurnberg, Henkestr. 9-11, 
      91054, Erlangen, Germany.
FAU - Goen, Thomas
AU  - Goen T
AD  - Institute and Outpatient Clinic of Occupational, Social and Environmental 
      Medicine, Friedrich-Alexander-Universitat Erlangen-Nurnberg, Henkestr. 9-11, 
      91054, Erlangen, Germany.
FAU - Eckert, Elisabeth
AU  - Eckert E
AD  - Institute and Outpatient Clinic of Occupational, Social and Environmental 
      Medicine, Friedrich-Alexander-Universitat Erlangen-Nurnberg, Henkestr. 9-11, 
      91054, Erlangen, Germany. elisabeth.eckert@fau.de.
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20180424
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Benzoates)
RN  - 0 (Plasticizers)
RN  - 3319-31-1 (tri-(2-ethylhexyl)trimellitate)
SB  - IM
MH  - Benzoates/metabolism/*urine
MH  - Calibration
MH  - Chromatography, Liquid/*methods
MH  - Environmental Monitoring/*methods
MH  - Humans
MH  - Limit of Detection
MH  - Pilot Projects
MH  - Plasticizers/*metabolism
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - Biomonitoring
OT  - Core-shell material
OT  - Human metabolism
OT  - Medical devices
OT  - Plasticizer
OT  - Tri-(2-ethylhexyl) trimellitate
EDAT- 2018/04/25 06:00
MHDA- 2018/08/15 06:00
CRDT- 2018/04/25 06:00
PHST- 2018/02/01 00:00 [received]
PHST- 2018/04/13 00:00 [accepted]
PHST- 2018/03/21 00:00 [revised]
PHST- 2018/04/25 06:00 [pubmed]
PHST- 2018/08/15 06:00 [medline]
PHST- 2018/04/25 06:00 [entrez]
AID - 10.1007/s00216-018-1086-7 [pii]
AID - 10.1007/s00216-018-1086-7 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2018 Jul;410(18):4343-4357. doi: 10.1007/s00216-018-1086-7. 
      Epub 2018 Apr 24.