PMID- 29687844
OWN - NLM
STAT- MEDLINE
DCOM- 20191107
LR  - 20220330
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 22
IP  - 7
DP  - 2018 Apr
TI  - Peroxiredoxin1 promotes cell proliferation, migration and invasion of colorectal 
      cancer via p38MAPK signaling.
PG  - 1922-1928
LID - 14715 [pii]
LID - 10.26355/eurrev_201804_14715 [doi]
AB  - OBJECTIVE: Peroxiredoxin1 (PRDX1), a class of thiol peroxidases, is a 
      multifunctional protein. We aimed at analyzing the effect of PRDX1 on 
      proliferation, apoptosis, migration and invasion of colorectal cancer and to 
      investigate the potential mechanism. MATERIALS AND METHODS: Western blot and PCR 
      were used to validate the silencing efficiency in SW480 cell by transfection of 
      PRDX1-siRNA. The cell proliferation was detected by Cell Counting Kit-8 (CCK-8) 
      test. Flow cytometry Annexin V/PI double staining was used to analyze cell 
      apoptosis. Transwell and scratch test were used to detect the migration and 
      invasion of cells. Signal pathway protein expression was analyzed by Western 
      blot. RESULTS: The expression of PRDX1 in SW480 cells could be reduced by siRNA 
      effectively. The cell proliferation, migration and invasion were reduced 
      significantly compared with control group after down-regulation of PRDX1 
      (p<0.05), while the cell apoptosis was enhanced significantly (p<0.05). The ratio 
      of phospho-p38 mitogen-activated protein kinases (p-p38) /p38 mitogen-activated 
      protein kinases (p38) was down-regulated after the down-regulation of PRDX1 
      (p<0.05). The ratio of phospho-c-Jun N-terminal protein kinase (p-JNK)/c-Jun 
      N-terminal protein kinase (JNK) and phospho-extracellular regulated protein 
      kinases (p-ERK)/extracellular regulated protein kinases (ERK) showed changes with 
      no significant difference (p>0.05). CONCLUSIONS: Down-regulation of PRDX1 in 
      colorectal cancer SW480 cells could inhibit the cell proliferation, migration, 
      invasion, and induce cell apoptosis. This is very likely to be achieved by 
      activating the p38MAPK-signaling pathway.
FAU - Cong, N
AU  - Cong N
AD  - Shandong University, Jinan, Shandong, China. baoshengli1963@163.com.
FAU - Huang, W
AU  - Huang W
FAU - Yuan, J-P
AU  - Yuan JP
FAU - Li, G-Z
AU  - Li GZ
FAU - Zhai, G-S
AU  - Zhai GS
FAU - Li, B-S
AU  - Li BS
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - EC 1.11.1.15 (PRDX1 protein, human)
RN  - EC 1.11.1.15 (Peroxiredoxins)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Apoptosis
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation/drug effects
MH  - Colorectal Neoplasms/*pathology
MH  - Humans
MH  - MAP Kinase Signaling System
MH  - Neoplasm Invasiveness
MH  - Peroxiredoxins/*physiology
MH  - p38 Mitogen-Activated Protein Kinases/*physiology
EDAT- 2018/04/25 06:00
MHDA- 2019/11/08 06:00
CRDT- 2018/04/25 06:00
PHST- 2018/04/25 06:00 [entrez]
PHST- 2018/04/25 06:00 [pubmed]
PHST- 2019/11/08 06:00 [medline]
AID - 14715 [pii]
AID - 10.26355/eurrev_201804_14715 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2018 Apr;22(7):1922-1928. doi: 
      10.26355/eurrev_201804_14715.