PMID- 29688091
OWN - NLM
STAT- MEDLINE
DCOM- 20191203
LR  - 20191203
IS  - 1742-8149 (Electronic)
IS  - 1464-7273 (Linking)
VI  - 22
IP  - 2
DP  - 2019 Jun
TI  - Molecular detection of HHV1-5, AAV and HPV in semen specimens and their impact on 
      male fertility.
PG  - 133-138
LID - 10.1080/14647273.2018.1463570 [doi]
AB  - Viral infections have been considered as possible destructive factors that 
      influence male fertility. The aim of this study was to determine the prevalence 
      of human herpes viruses 1-5 (HHV1-5), adeno associated virus (AAV) and human 
      papilloma virus (HPV) in semen and whether these influence semen quality. DNA 
      extraction was performed using phenol-chloroform protocol, then three different 
      nested-PCRs were done to detect HHV1-5, AAV and HPV DNAs in the semen samples. Of 
      145 samples, 66 (45.5%) were positive at least for one of the viruses. The genome 
      detection rate of HSV1/2, VZV, EBV, HCMV, AAV and HPV were zero, 2.8%, zero, 
      1.4%, 27.6% and 19.3%, respectively. Of 66 positive samples for these viruses, 6 
      (4.1% of all samples) were positive for two viruses simultaneously. Here no 
      association was found between variations in semen parameters related to fertility 
      and detection of VZV, HCMV, AAV and HPV DNA in semen samples. It should be noted 
      that the prevalence of different viruses in semen, and their relevance to male 
      infertility, differs significantly due to the genome extraction and amplification 
      methods or due to a real variation between study populations and geographical 
      regions.
FAU - Behboudi, Emad
AU  - Behboudi E
AD  - a Virology Department, School of Public Health , Tehran University of Medical 
      Sciences , Tehran , Iran.
FAU - Mokhtari-Azad, Talat
AU  - Mokhtari-Azad T
AD  - a Virology Department, School of Public Health , Tehran University of Medical 
      Sciences , Tehran , Iran.
FAU - Yavarian, Jila
AU  - Yavarian J
AD  - a Virology Department, School of Public Health , Tehran University of Medical 
      Sciences , Tehran , Iran.
FAU - Ghavami, Nastaran
AU  - Ghavami N
AD  - a Virology Department, School of Public Health , Tehran University of Medical 
      Sciences , Tehran , Iran.
FAU - Seyed Khorrami, Seyed Mahmood
AU  - Seyed Khorrami SM
AD  - b Department of Virology, Faculty of Medical Sciences , Tarbiat Modares 
      University , Tehran , Iran.
FAU - Rezaei, Farhad
AU  - Rezaei F
AD  - a Virology Department, School of Public Health , Tehran University of Medical 
      Sciences , Tehran , Iran.
FAU - Charostad, Javad
AU  - Charostad J
AD  - a Virology Department, School of Public Health , Tehran University of Medical 
      Sciences , Tehran , Iran.
FAU - Shatizadeh Malekshahi, Somayeh
AU  - Shatizadeh Malekshahi S
AD  - b Department of Virology, Faculty of Medical Sciences , Tarbiat Modares 
      University , Tehran , Iran.
FAU - Shafiei-Jandaghi, Nazanin Zahra
AU  - Shafiei-Jandaghi NZ
AD  - a Virology Department, School of Public Health , Tehran University of Medical 
      Sciences , Tehran , Iran.
LA  - eng
PT  - Journal Article
DEP - 20180424
PL  - England
TA  - Hum Fertil (Camb)
JT  - Human fertility (Cambridge, England)
JID - 100888143
SB  - IM
MH  - Cross-Sectional Studies
MH  - Dependovirus/*isolation & purification
MH  - Herpesviridae/*isolation & purification
MH  - Humans
MH  - Infertility, Male/*virology
MH  - Iran/epidemiology
MH  - Male
MH  - Papillomaviridae/*isolation & purification
MH  - Semen/*virology
MH  - Virus Diseases/*complications/epidemiology
OTO - NOTNLM
OT  - AAV
OT  - HHV1-5
OT  - HPV
OT  - Semen
OT  - male infertility
EDAT- 2018/04/25 06:00
MHDA- 2019/12/04 06:00
CRDT- 2018/04/25 06:00
PHST- 2018/04/25 06:00 [pubmed]
PHST- 2019/12/04 06:00 [medline]
PHST- 2018/04/25 06:00 [entrez]
AID - 10.1080/14647273.2018.1463570 [doi]
PST - ppublish
SO  - Hum Fertil (Camb). 2019 Jun;22(2):133-138. doi: 10.1080/14647273.2018.1463570. 
      Epub 2018 Apr 24.