PMID- 29688694
OWN - NLM
STAT- MEDLINE
DCOM- 20190417
LR  - 20191210
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 90
IP  - 10
DP  - 2018 May 15
TI  - In Situ Determination of Illegal Drugs in Oral Fluid by Portable Capillary 
      Electrophoresis with Deep UV Excited Fluorescence Detection.
PG  - 6253-6258
LID - 10.1021/acs.analchem.8b00911 [doi]
AB  - The present study demonstrates the potential of a portable capillary 
      electrophoresis (CE) instrument, coupled to deep UV fluorescence detector (FD) 
      with a 230-255 nm excitation wavelength range, for the determination of the abuse 
      of illegal drugs in oral fluids in situ. CE was introduced in this study due its 
      exceptional power of separation and resolution, short analysis time, and ability 
      for miniaturization for on-site assessment of different substances. The deep UV 
      fluorescence detector was equipped with five interchangeable emission filters, in 
      the emission wavelength range from 278 to 600 nm, and was successfully employed 
      for determination of natively fluorescing illegal drugs, such as cocaine, 
      cocaethylene, 3,4-methylenedioxymethamphetamine (MDMA), 
      3,4-methylenedioxeamphetamine (MDA), 3,4-methylenedioxy- N-ethylamphetamine 
      (MDEA), para-methoxyamphetamine (PMA), para-methoxy- N-methylamphetamine (PMMA), 
      amphetamine (AMP), methamphetamine (METH), tetrahydrocannabinol (THC) and 
      cannabidiol (CBD). The developed FD showed impressive sensitivity. The 
      instrumental detection limit was 0.5 mug/L for MDMA. It also showed broad 
      linearity, up to 50 mg/L for MDMA. The noise CV% was 1.1% for an empty capillary 
      and 0.6% for a capillary filled with acetonitrile. The portable CE-FD with 
      developed electrophoretic methodologies was successfully utilized for the 
      determination of illegal abuse of drugs during "Weekend" 2016 and 2017 Music 
      Festivals (Estonia). Moreover, CE-FD can be employed for detection of other 
      natively fluorescing compounds in the proposed range (e.g., for different 
      phenolic compounds, BTEX, naphthalene derivatives, and others), significantly 
      widening the applicability of developed CE-FD instrument.
FAU - Saar-Reismaa, Piret
AU  - Saar-Reismaa P
AD  - Department of Chemistry and Biotechnology, Faculty of Science , Tallinn 
      University of Technology , Akadeemia tee 15 , 12618 , Tallinn , Estonia.
FAU - Erme, Enn
AU  - Erme E
AD  - Omec OU , Riia str 185 , 51014 , Tartu , Estonia.
FAU - Vaher, Merike
AU  - Vaher M
AD  - Department of Chemistry and Biotechnology, Faculty of Science , Tallinn 
      University of Technology , Akadeemia tee 15 , 12618 , Tallinn , Estonia.
FAU - Kulp, Maria
AU  - Kulp M
AD  - Department of Chemistry and Biotechnology, Faculty of Science , Tallinn 
      University of Technology , Akadeemia tee 15 , 12618 , Tallinn , Estonia.
FAU - Kaljurand, Mihkel
AU  - Kaljurand M
AD  - Department of Chemistry and Biotechnology, Faculty of Science , Tallinn 
      University of Technology , Akadeemia tee 15 , 12618 , Tallinn , Estonia.
FAU - Mazina-Sinkar, Jekaterina
AU  - Mazina-Sinkar J
AD  - Department of Chemistry and Biotechnology, Faculty of Science , Tallinn 
      University of Technology , Akadeemia tee 15 , 12618 , Tallinn , Estonia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180504
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Illicit Drugs)
SB  - IM
MH  - Electrophoresis, Capillary/*methods
MH  - Equipment Design
MH  - *Fluorescence
MH  - Humans
MH  - Illicit Drugs/*analysis
MH  - Saliva/*chemistry
MH  - Substance Abuse Detection/instrumentation/*methods
MH  - *Ultraviolet Rays
EDAT- 2018/04/25 06:00
MHDA- 2019/04/18 06:00
CRDT- 2018/04/25 06:00
PHST- 2018/04/25 06:00 [pubmed]
PHST- 2019/04/18 06:00 [medline]
PHST- 2018/04/25 06:00 [entrez]
AID - 10.1021/acs.analchem.8b00911 [doi]
PST - ppublish
SO  - Anal Chem. 2018 May 15;90(10):6253-6258. doi: 10.1021/acs.analchem.8b00911. Epub 
      2018 May 4.