PMID- 29688842
OWN - NLM
STAT- MEDLINE
DCOM- 20181217
LR  - 20181217
IS  - 1471-2164 (Electronic)
IS  - 1471-2164 (Linking)
VI  - 19
IP  - 1
DP  - 2018 Apr 23
TI  - Partial-arm translocations in evolution of malaria mosquitoes revealed by 
      high-coverage physical mapping of the Anopheles atroparvus genome.
PG  - 278
LID - 10.1186/s12864-018-4663-4 [doi]
LID - 278
AB  - BACKGROUND: Malaria mosquitoes have had a remarkable stability in the number of 
      chromosomes in their karyotype (2n = 6) during 100 million years of evolution. 
      Moreover, autosomal arms were assumed to maintain their integrity even if their 
      associations with each other changed via whole-arm translocations. Here we use 
      high-coverage comparative physical genome mapping of three Anopheles species to 
      test the extent of evolutionary conservation of chromosomal arms in malaria 
      mosquitoes. RESULTS: In this study, we developed a physical genome map for 
      Anopheles atroparvus, one of the dominant malaria vectors in Europe. Using 
      fluorescence in situ hybridization (FISH) of DNA probes with the ovarian nurse 
      cell polytene chromosomes and synteny comparison, we anchored 56 genomic 
      scaffolds to the An. atroparvus chromosomes. The obtained physical map represents 
      89.6% of the An. atroparvus genome. This genome has the second highest mapping 
      coverage among Anophelinae assemblies after An. albimanus, which has 98.2% of the 
      genome assigned to its chromosomes. A comparison of the An. atroparvus, An. 
      albimanus, and An. gambiae genomes identified partial-arm translocations between 
      the autosomal arms that break down the integrity of chromosome elements in 
      evolution affecting the structure of the genetic material in the pericentromeric 
      regions. Unlike An. atroparvus and An. albimanus, all chromosome elements of An. 
      gambiae are fully syntenic with chromosome elements of the putative ancestral 
      Anopheles karyotype. We also detected nonrandom distribution of large conserved 
      synteny blocks and confirmed a higher rate of inversion fixation in the X 
      chromosome compared with autosomes. CONCLUSIONS: Our study demonstrates the power 
      of physical mapping for understanding the genome evolution in malaria mosquitoes. 
      The results indicate that syntenic relationships among chromosome elements of 
      Anopheles species have not been fully preserved because of multiple partial-arm 
      translocations.
FAU - Artemov, Gleb N
AU  - Artemov GN
AD  - Laboratory of Ecology, Genetics and Environmental Protection, Tomsk State 
      University, 36 Lenin Avenue, Tomsk, 634050, Russia.
FAU - Bondarenko, Semen M
AU  - Bondarenko SM
AD  - Laboratory of Ecology, Genetics and Environmental Protection, Tomsk State 
      University, 36 Lenin Avenue, Tomsk, 634050, Russia.
FAU - Naumenko, Anastasia N
AU  - Naumenko AN
AD  - Department of Entomology, Fralin Life Science Institute, Virginia Polytechnic 
      Institute and State University, 360 West Campus Drive, Blacksburg, VA, 24061, 
      USA.
FAU - Stegniy, Vladimir N
AU  - Stegniy VN
AD  - Laboratory of Ecology, Genetics and Environmental Protection, Tomsk State 
      University, 36 Lenin Avenue, Tomsk, 634050, Russia.
FAU - Sharakhova, Maria V
AU  - Sharakhova MV
AD  - Laboratory of Ecology, Genetics and Environmental Protection, Tomsk State 
      University, 36 Lenin Avenue, Tomsk, 634050, Russia. msharakh@vt.edu.
AD  - Department of Entomology, Fralin Life Science Institute, Virginia Polytechnic 
      Institute and State University, 360 West Campus Drive, Blacksburg, VA, 24061, 
      USA. msharakh@vt.edu.
FAU - Sharakhov, Igor V
AU  - Sharakhov IV
AUID- ORCID: 0000-0003-0752-3747
AD  - Laboratory of Ecology, Genetics and Environmental Protection, Tomsk State 
      University, 36 Lenin Avenue, Tomsk, 634050, Russia. igor@vt.edu.
AD  - Department of Entomology, Fralin Life Science Institute, Virginia Polytechnic 
      Institute and State University, 360 West Campus Drive, Blacksburg, VA, 24061, 
      USA. igor@vt.edu.
LA  - eng
GR  - 15-14-20011/Russian Science Foundation/
PT  - Journal Article
DEP - 20180423
PL  - England
TA  - BMC Genomics
JT  - BMC genomics
JID - 100965258
SB  - IM
MH  - Animals
MH  - Anopheles/*genetics/physiology
MH  - *Evolution, Molecular
MH  - Female
MH  - Genome, Insect/*genetics
MH  - *Malaria
MH  - Ovary/cytology
MH  - *Physical Chromosome Mapping
MH  - Synteny
MH  - Translocation, Genetic/*genetics
PMC - PMC5914054
OTO - NOTNLM
OT  - Anopheles atroparvus
OT  - Chromosome evolution
OT  - Fluorescence in situ hybridization
OT  - Mosquito genome
OT  - Partial-arm translocation
OT  - Physical mapping
OT  - Polytene chromosomes
COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: Not applicable COMPETING INTERESTS: 
      The authors declare that they have no competing interests. PUBLISHER'S NOTE: 
      Springer Nature remains neutral with regard to jurisdictional claims in published 
      maps and institutional affiliations.
EDAT- 2018/04/25 06:00
MHDA- 2018/12/18 06:00
PMCR- 2018/04/23
CRDT- 2018/04/25 06:00
PHST- 2017/10/26 00:00 [received]
PHST- 2018/04/12 00:00 [accepted]
PHST- 2018/04/25 06:00 [entrez]
PHST- 2018/04/25 06:00 [pubmed]
PHST- 2018/12/18 06:00 [medline]
PHST- 2018/04/23 00:00 [pmc-release]
AID - 10.1186/s12864-018-4663-4 [pii]
AID - 4663 [pii]
AID - 10.1186/s12864-018-4663-4 [doi]
PST - epublish
SO  - BMC Genomics. 2018 Apr 23;19(1):278. doi: 10.1186/s12864-018-4663-4.