PMID- 29689458
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1936-5233 (Print)
IS  - 1936-5233 (Electronic)
IS  - 1936-5233 (Linking)
VI  - 11
IP  - 3
DP  - 2018 Jun
TI  - Cytoplasmic TrkA Expression as a Screen for Detecting NTRK1 Fusions in Colorectal 
      Cancer.
PG  - 764-770
LID - S1936-5233(18)30047-0 [pii]
LID - 10.1016/j.tranon.2018.03.011 [doi]
AB  - NTRK1 gene fusions, the targets of multikinase inhibitors, are promising 
      therapeutic targets for colorectal cancer (CRC). However, screening methods for 
      detecting NTRK1 gene fusions in CRC tissues have not been reported. In this 
      study, we investigated the potential use of immunohistochemistry (IHC) for 
      detecting NTRK1 gene fusions. We performed and compared IHC with fluorescence in 
      situ hybridization (FISH) in 80 CRC patients. TrkA immunostaining was observed to 
      be both membranous and cytoplasmic and was scored semiquantitatively using 
      staining intensity and proportions. The tumors were observed to be NTRK1 gene 
      fusion-positive when >/=20 out of 100 nuclei in FISH. A significant correlation 
      between the IHC and FISH results for determination of the NTRK1 gene fusions was 
      observed. We measured the cytoplasmic TrkA expression, which showed an area under 
      the receiver operating characteristic (ROC) curve of 0.926 (range: 0.864-0.987, 
      95% CI, P=.001). By choosing 4.5 (sum of the intensity and proportion scores of 
      cytoplasmic TrkA expression) as the cut-off value for the positive and negative 
      NTRK1 gene fusion groups, the sensitivity and specificity for predicting lymph 
      node metastasis were 100 and 83.8%, respectively (P=.001). Specifically, high 
      cytoplasmic TrkA expression (sum of intensity and proportion scores >4) was 
      associated with the presence of NTRK1 gene fusions (P<.0001, r=0.528). Taken 
      together, our data showed that IHC for TrkA can be used as an efficient screening 
      method for detecting NTRK1 gene fusions in CRC.
CI  - Copyright (c) 2018 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Choi, Yuri
AU  - Choi Y
AD  - Department of Pathology, Pusan National University Hospital and Pusan National 
      University School of Medicine, and Biomedical Research Institute, Pusan National 
      University Hospital, 179 Gudeok-ro, Seo-Gu, Busan 49241, Republic of Korea.
FAU - Won, Yeo-Jin
AU  - Won YJ
AD  - Department of Pathology, Pusan National University Hospital and Pusan National 
      University School of Medicine, and Biomedical Research Institute, Pusan National 
      University Hospital, 179 Gudeok-ro, Seo-Gu, Busan 49241, Republic of Korea.
FAU - Lee, Sojeong
AU  - Lee S
AD  - Department of Pathology, Pusan National University Hospital and Pusan National 
      University School of Medicine, and Biomedical Research Institute, Pusan National 
      University Hospital, 179 Gudeok-ro, Seo-Gu, Busan 49241, Republic of Korea.
FAU - Kim, Ahrong
AU  - Kim A
AD  - Department of Pathology, Pusan National University Hospital and Pusan National 
      University School of Medicine, and Biomedical Research Institute, Pusan National 
      University Hospital, 179 Gudeok-ro, Seo-Gu, Busan 49241, Republic of Korea.
FAU - Kim, Younggeum
AU  - Kim Y
AD  - Department of Pathology, Pusan National University Hospital and Pusan National 
      University School of Medicine, and Biomedical Research Institute, Pusan National 
      University Hospital, 179 Gudeok-ro, Seo-Gu, Busan 49241, Republic of Korea.
FAU - Park, Won-Young
AU  - Park WY
AD  - Department of Pathology, Pusan National University Hospital and Pusan National 
      University School of Medicine, and Biomedical Research Institute, Pusan National 
      University Hospital, 179 Gudeok-ro, Seo-Gu, Busan 49241, Republic of Korea.
FAU - Jo, Hong-Jae
AU  - Jo HJ
AD  - Department of Surgery, Pusan National University Hospital and Pusan National 
      University School of Medicine, and Biomedical Research Institute, Pusan National 
      University Hospital, 179 Gudeok-ro, Seo-Gu, Busan 49241, Republic of Korea.
FAU - Song, Geun Am
AU  - Song GA
AD  - Department of Internal Medicine, Pusan National University Hospital and Pusan 
      National University School of Medicine, and Biomedical Research Institute, Pusan 
      National University Hospital, 179 Gudeok-ro, Seo-Gu, Busan 49241, Republic of 
      Korea.
FAU - Kwon, Chae Hwa
AU  - Kwon CH
AD  - Department of Pathology, Pusan National University Hospital and Pusan National 
      University School of Medicine, and Biomedical Research Institute, Pusan National 
      University Hospital, 179 Gudeok-ro, Seo-Gu, Busan 49241, Republic of Korea.
FAU - Park, Do Youn
AU  - Park DY
AD  - Department of Pathology, Pusan National University Hospital and Pusan National 
      University School of Medicine, and Biomedical Research Institute, Pusan National 
      University Hospital, 179 Gudeok-ro, Seo-Gu, Busan 49241, Republic of Korea. 
      Electronic address: pdy220@pusan.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20180422
PL  - United States
TA  - Transl Oncol
JT  - Translational oncology
JID - 101472619
PMC - PMC6050437
EDAT- 2018/04/25 06:00
MHDA- 2018/04/25 06:01
PMCR- 2018/04/22
CRDT- 2018/04/25 06:00
PHST- 2018/02/02 00:00 [received]
PHST- 2018/03/28 00:00 [revised]
PHST- 2018/03/28 00:00 [accepted]
PHST- 2018/04/25 06:00 [pubmed]
PHST- 2018/04/25 06:01 [medline]
PHST- 2018/04/25 06:00 [entrez]
PHST- 2018/04/22 00:00 [pmc-release]
AID - S1936-5233(18)30047-0 [pii]
AID - 10.1016/j.tranon.2018.03.011 [doi]
PST - ppublish
SO  - Transl Oncol. 2018 Jun;11(3):764-770. doi: 10.1016/j.tranon.2018.03.011. Epub 
      2018 Apr 22.