PMID- 29691704
OWN - NLM
STAT- MEDLINE
DCOM- 20180814
LR  - 20180814
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 163
IP  - 8
DP  - 2018 Aug
TI  - Different susceptibility to porcine reproductive and respiratory syndrome virus 
      infection among Chinese native pig breeds.
PG  - 2155-2164
LID - 10.1007/s00705-018-3821-y [doi]
AB  - China is rich in native pig breeds, yet information regarding the 
      susceptibility/resistance of local breeds to porcine reproductive and respiratory 
      syndrome virus (PRRSV) infection is lacking. In the present study, an in vitro 
      method based on assessing PRRSV replication in porcine alveolar macrophages 
      (PAMs) was established to evaluate PRRSV susceptibility/resistance in a 
      commercial pig breed (Landrace) and five native pig breeds from Jiangsu and Anhui 
      provinces in China. Expression levels of cytokines (IL-8, IL-10, TNF-alpha and 
      IFN-gamma), Toll-like receptor 3 (TLR3), CD163 (PRRSV receptor), and sialoadhesin 
      (Sn, PRRSV receptor) in infected pigs were determined using real-time PCR, and 
      the association between PRRSV susceptibility/resistance and the abundance of the 
      cytokines and receptors was investigated. The viral replication rate and titer at 
      0, 6, 12 18, 24 and 36 hours postinfection (hpi) were determined to assess the 
      proliferation dynamics of PRRSV NJGC in PAMs. Based on the PRRSV proliferation 
      dynamics, the results indicated that Dingyuan pigs were the most susceptible to 
      PRRSV infection, whereas Jiangquhai pigs were the least susceptible to PRRSV 
      infection among the six pig breeds tested, as indicated by measuring PRRSV 
      replication and the viral load in PAMs. The different levels of susceptibility to 
      PRRSV infection in PAMs may be associated with differences in the abundance of 
      CD163 (PRRSV receptor), cytokines IL-8, IFN-gamma, and TNF-alpha in Jiangquhai and 
      Dingyuan pig breeds after viral inoculation.
FAU - Meng, Chunhua
AU  - Meng C
AD  - Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, 
      210014, Jiangsu, China.
AD  - Key Laboratory of Animal Breeding and Reproduction, Jiangsu Academy of 
      Agricultural Sciences, Nanjing, 210014, China.
FAU - Su, Lei
AU  - Su L
AD  - Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, 
      210014, Jiangsu, China.
AD  - College of Animal Science and Technology, Nanjing Agricultural University, 
      Nanjing, 210095, Jiangsu, China.
FAU - Li, Yinxia
AU  - Li Y
AD  - Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, 
      210014, Jiangsu, China.
AD  - Key Laboratory of Animal Breeding and Reproduction, Jiangsu Academy of 
      Agricultural Sciences, Nanjing, 210014, China.
FAU - Zhu, Qianming
AU  - Zhu Q
AD  - College of Animal Science and Technology, Nanjing Agricultural University, 
      Nanjing, 210095, Jiangsu, China.
FAU - Li, Jingxin
AU  - Li J
AD  - College of Animal Science and Technology, Nanjing Agricultural University, 
      Nanjing, 210095, Jiangsu, China.
FAU - Wang, Huili
AU  - Wang H
AD  - Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, 
      210014, Jiangsu, China.
AD  - Key Laboratory of Animal Breeding and Reproduction, Jiangsu Academy of 
      Agricultural Sciences, Nanjing, 210014, China.
FAU - He, Qiang
AU  - He Q
AD  - Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, 
      210014, Jiangsu, China.
FAU - Wang, Chunling
AU  - Wang C
AD  - College of Animal Science and Technology, Nanjing Agricultural University, 
      Nanjing, 210095, Jiangsu, China.
FAU - Wang, Wei
AU  - Wang W
AD  - College of Animal Science and Technology, Nanjing Agricultural University, 
      Nanjing, 210095, Jiangsu, China.
FAU - Cao, Shaoxian
AU  - Cao S
AUID- ORCID: 0000-0003-3391-2025
AD  - Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, 
      210014, Jiangsu, China. sxcao@jaas.ac.cn.
AD  - Key Laboratory of Animal Breeding and Reproduction, Jiangsu Academy of 
      Agricultural Sciences, Nanjing, 210014, China. sxcao@jaas.ac.cn.
AD  - College of Animal Science and Technology, Nanjing Agricultural University, 
      Nanjing, 210095, Jiangsu, China. sxcao@jaas.ac.cn.
LA  - eng
GR  - 30972075/National Natural Science Foundation of China/
GR  - CX (12)2034/Jiangsu Agriculture Science and Technology Innovation Fund/
GR  - BK20140741/Natural Science Foundation of Jiangsu Province/
PT  - Comparative Study
PT  - Journal Article
DEP - 20180424
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD163 antigen)
RN  - 0 (Cytokines)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Antigens, CD/genetics/immunology
MH  - Antigens, Differentiation, Myelomonocytic/genetics/immunology
MH  - Breeding
MH  - China
MH  - Cytokines/genetics/immunology
MH  - Female
MH  - Macrophages, Alveolar/immunology/virology
MH  - Male
MH  - Porcine Reproductive and Respiratory Syndrome/genetics/immunology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*physiology
MH  - Receptors, Cell Surface/genetics/immunology
MH  - Swine
MH  - Tumor Necrosis Factor-alpha/genetics/immunology
MH  - Virus Replication
EDAT- 2018/04/25 06:00
MHDA- 2018/08/15 06:00
CRDT- 2018/04/26 06:00
PHST- 2017/09/07 00:00 [received]
PHST- 2018/03/14 00:00 [accepted]
PHST- 2018/04/25 06:00 [pubmed]
PHST- 2018/08/15 06:00 [medline]
PHST- 2018/04/26 06:00 [entrez]
AID - 10.1007/s00705-018-3821-y [pii]
AID - 10.1007/s00705-018-3821-y [doi]
PST - ppublish
SO  - Arch Virol. 2018 Aug;163(8):2155-2164. doi: 10.1007/s00705-018-3821-y. Epub 2018 
      Apr 24.