PMID- 29696534
OWN - NLM
STAT- MEDLINE
DCOM- 20190508
LR  - 20190508
IS  - 1559-0720 (Electronic)
IS  - 0163-4984 (Linking)
VI  - 187
IP  - 1
DP  - 2019 Jan
TI  - Ascorbic Acid Attenuates Lead-Induced Alterations in the Synapses in the 
      Developing Rat Cerebellum.
PG  - 142-150
LID - 10.1007/s12011-018-1354-6 [doi]
AB  - We evaluated the effect of lead (Pb) and ascorbic acid treatment of pregnant 
      female rats on cerebellar development in pups. Pb was administered in drinking 
      water (0.2% Pb acetate), and ascorbic acid (100 mg/kg) was administered through 
      oral intubation. Fifteen female rats were randomly classified into control, Pb, 
      and Pb plus ascorbic acid (PA) groups. The treatment of Pb and ascorbic acid 
      treatments were terminated after birth to evaluate the effects on the gestational 
      development of the cerebellum. At postnatal day 21 (PND21), pups were sacrificed, 
      and blood Pb level was analyzed. Blood Pb levels of pups and dams were highest in 
      the Pb group and reduced in the PA group. Immunohistochemistry and immunoblot 
      assays were conducted to study the cerebellar expression levels of synaptic 
      proteins. Along with a significant reduction in Purkinje cells, the reduction in 
      presynaptic (synaptophysin) and postsynaptic (postsynaptic density protein 95, 
      N-methyl-D-aspartate receptor subtype 1) marker proteins was observed in 
      Pb-exposed pups. Ascorbic acid treatment significantly prevented Pb-induced 
      impairment in the cerebellar synaptic proteins. Hypothesizing that brain-derived 
      neurotrophic factor (BDNF) might be affected by Pb exposure given its importance 
      in the regulation of synaptogenesis, we observed a Pb-induced decrease and 
      ascorbic acid-mediated increase of BDNF in the cerebellum. Luxol fast blue 
      staining and myelin basic protein analysis suggest that ascorbic acid treatment 
      ameliorated the Pb exposure-induced reduction in the axonal fibers in the 
      developing cerebellum. Overall, we conclude that ascorbic acid treatment during 
      pregnancy can prevent Pb-induced impairments in the cerebellar development in 
      rats.
FAU - Nam, Sung Min
AU  - Nam SM
AD  - Department of Anatomy, College of Veterinary Medicine, Konkuk University, 120 
      Neungdong-ro, Gwangjin-gu, Seoul, 05030, Republic of Korea.
AD  - College of Veterinary Medicine and Veterinary Science Research Institute, Konkuk 
      University, Seoul, 05030, Republic of Korea.
FAU - Cho, In-Sun
AU  - Cho IS
AD  - Department of Anatomy, College of Veterinary Medicine, Konkuk University, 120 
      Neungdong-ro, Gwangjin-gu, Seoul, 05030, Republic of Korea.
AD  - Korea Bio-Safety Institute Co. Ltd, Eumseong, Chungbuk, 27600, Republic of Korea.
FAU - Seo, Jin Seok
AU  - Seo JS
AD  - Department of Anatomy, College of Veterinary Medicine, Konkuk University, 120 
      Neungdong-ro, Gwangjin-gu, Seoul, 05030, Republic of Korea.
FAU - Go, Tae-Hun
AU  - Go TH
AD  - Department of Anatomy, College of Veterinary Medicine, Konkuk University, 120 
      Neungdong-ro, Gwangjin-gu, Seoul, 05030, Republic of Korea.
FAU - Kim, Ji-Hye
AU  - Kim JH
AD  - Department of Rehabilitation Psychology, Seoul Rehabilitation Hospital, Seoul, 
      03428, Republic of Korea.
FAU - Nahm, Sang-Soep
AU  - Nahm SS
AD  - Department of Anatomy, College of Veterinary Medicine, Konkuk University, 120 
      Neungdong-ro, Gwangjin-gu, Seoul, 05030, Republic of Korea.
AD  - College of Veterinary Medicine and Veterinary Science Research Institute, Konkuk 
      University, Seoul, 05030, Republic of Korea.
FAU - Chang, Byung-Joon
AU  - Chang BJ
AD  - Department of Anatomy, College of Veterinary Medicine, Konkuk University, 120 
      Neungdong-ro, Gwangjin-gu, Seoul, 05030, Republic of Korea. bjchang@konkuk.ac.kr.
AD  - College of Veterinary Medicine and Veterinary Science Research Institute, Konkuk 
      University, Seoul, 05030, Republic of Korea. bjchang@konkuk.ac.kr.
FAU - Lee, Jong-Hwan
AU  - Lee JH
AD  - Department of Anatomy, College of Veterinary Medicine, Konkuk University, 120 
      Neungdong-ro, Gwangjin-gu, Seoul, 05030, Republic of Korea. jhlee21@konkuk.ac.kr.
AD  - College of Veterinary Medicine and Veterinary Science Research Institute, Konkuk 
      University, Seoul, 05030, Republic of Korea. jhlee21@konkuk.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20180426
PL  - United States
TA  - Biol Trace Elem Res
JT  - Biological trace element research
JID - 7911509
RN  - 2P299V784P (Lead)
RN  - PQ6CK8PD0R (Ascorbic Acid)
SB  - IM
MH  - Administration, Oral
MH  - Animals
MH  - Animals, Newborn
MH  - Ascorbic Acid/administration & dosage/*pharmacology
MH  - Cerebellum/*drug effects/*growth & development/metabolism
MH  - Female
MH  - Lead/administration & dosage/*toxicity
MH  - Male
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Synapses/*drug effects/metabolism
OTO - NOTNLM
OT  - Ascorbic acid
OT  - Cerebellum
OT  - Lead (Pb)
OT  - NMDAR1
OT  - PSD95
OT  - Synaptophysin
EDAT- 2018/04/27 06:00
MHDA- 2019/05/09 06:00
CRDT- 2018/04/27 06:00
PHST- 2018/03/12 00:00 [received]
PHST- 2018/04/17 00:00 [accepted]
PHST- 2018/04/27 06:00 [pubmed]
PHST- 2019/05/09 06:00 [medline]
PHST- 2018/04/27 06:00 [entrez]
AID - 10.1007/s12011-018-1354-6 [pii]
AID - 10.1007/s12011-018-1354-6 [doi]
PST - ppublish
SO  - Biol Trace Elem Res. 2019 Jan;187(1):142-150. doi: 10.1007/s12011-018-1354-6. 
      Epub 2018 Apr 26.