PMID- 29696925
OWN - NLM
STAT- MEDLINE
DCOM- 20180815
LR  - 20181202
IS  - 0255-2930 (Print)
IS  - 0255-2930 (Linking)
VI  - 38
IP  - 4
DP  - 2018 Apr 12
TI  - [Effects of electroacupuncture at "Changqiang" (GV 1) on expression of nerve 
      growth factor and brain derived neurotrophic factor in rats after acute spinal 
      cord injury].
PG  - 399-404
LID - 10.13703/j.0255-2930.2018.04.015 [doi]
AB  - OBJECTIVE: To observe the effects of electroacupuncture (EA) at"Changqiang"(GV 1) 
      on expression of nerve growth factor (NGF) and brain derived neurotrophic factor 
      (BDNF) in rats after acute spinal cord injury (ASCI), and to explore the 
      mechanism of EA at"Changqiang"(GV 1) on ASCI. METHODS: Twenty-four adult female 
      SD rats were randomly divided into an EA group, a model group and a sham 
      operation group, 8 rats in each one. The rats in the sham operation group were 
      treated with laminectomy to expose the spinal cord without any strike. The rats 
      in the model group and EA group were treated with modified Allen's method to 
      establish ASCI model. After model was established, the rats in the EA group were 
      treated with EA at"Changqiang"(GV 1), once a day for continuous 7 days. The rats 
      in the sham operation group and model group were treated with immobilization, 
      once a day, without any other interventions. The basso beattie bresnahan (BBB) 
      was evaluated 1, 3, 5, 7 days after operation. 7 days after operation, the rats 
      were sacrificed with perfusion and the spinal cord was embedded with paraffin. 
      The morphological changes of spinal cord and neuron were observed by Nissl's 
      staining method; the expressions of NGF and BDNF were detected by immune 
      fluorescence method. RESULTS: 3 days, 5 days and 7 days after operation, the BBB 
      scores in the EA group were higher than those in the model group (P<0.05, 
      P<0.01). The Nissl's staining indicated the gray matter of spinal cord was 
      butterfly-shaped with complete structure and clear boundaries between the gray 
      and white matter; the tabby-shaped Nissl bodies were observed in cytoplasm. There 
      were incomplete gray nucleus, big and saturate local stasis plaque. Compared with 
      the model group, the smallerarea of blood stasis, less severity of neuron edema, 
      better morphology of neuron and no vacuole change were observed in the EA group. 
      The immune fluorescence results indicated the expressions of NGF and BDNF in the 
      model group and EA group were higher than those in the sham operation group (all 
      P<0.01); the expressions of NGF and BDNF in the EA group were higher than those 
      in the model group (both P<0.01). CONCLUSION: EA at"Changqiang"(GV 1) could 
      improve the expression of NGF and BDNF and increase the score of BBB in rats with 
      ASCI, which is beneficial to the repair of ASCI.
FAU - Huang, Zhiqiang
AU  - Huang Z
AD  - Fujian University of TCM, Fuzhou 350122, China; Quanzhou TCM Hospital Affiliated 
      to Fujian University of TCM, Quanzhou 362000.
FAU - Liu, Yu
AU  - Liu Y
AD  - Fujian University of TCM, Fuzhou 350122, China.
FAU - Su, Zhaoyuan
AU  - Su Z
AD  - Quanzhou TCM Hospital Affiliated to Fujian University of TCM, Quanzhou 362000.
FAU - Su, Jiafu
AU  - Su J
AD  - Quanzhou TCM Hospital Affiliated to Fujian University of TCM, Quanzhou 362000.
FAU - Wu, Qiang
AU  - Wu Q
AD  - Fujian University of TCM, Fuzhou 350122, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhen Jiu
JT  - Zhongguo zhen jiu = Chinese acupuncture & moxibustion
JID - 8600658
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - *Electroacupuncture
MH  - Female
MH  - Nerve Growth Factor/*metabolism
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord/*metabolism
MH  - Spinal Cord Injuries/*therapy
OTO - NOTNLM
OT  - Point GV 1 (Changqiang)
OT  - acute spinal cord injury
OT  - brain derived neurotrophic factor (BDNF)
OT  - electroacupuncture (EA)
OT  - nerve growth factor (NGF)
EDAT- 2018/04/27 06:00
MHDA- 2018/08/16 06:00
CRDT- 2018/04/27 06:00
PHST- 2018/04/27 06:00 [entrez]
PHST- 2018/04/27 06:00 [pubmed]
PHST- 2018/08/16 06:00 [medline]
AID - 10.13703/j.0255-2930.2018.04.015 [doi]
PST - ppublish
SO  - Zhongguo Zhen Jiu. 2018 Apr 12;38(4):399-404. doi: 
      10.13703/j.0255-2930.2018.04.015.