PMID- 29698763
OWN - NLM
STAT- MEDLINE
DCOM- 20181010
LR  - 20181010
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 115
DP  - 2018 Aug
TI  - Exploring the DNA damaging potential of chitosan and citrate-reduced gold 
      nanoparticles: Physicochemical approach.
PG  - 801-810
LID - S0141-8130(18)31220-0 [pii]
LID - 10.1016/j.ijbiomac.2018.04.115 [doi]
AB  - Nanomaterials offer a wide range of biomedical applications including gene/drug 
      delivery, biosensing and bioimaging. The cytotoxic and genotoxic potential of 
      nanoparticles need to be thoroughly investigated before their biomedical usage. 
      This study aims to investigate and compare the nanotoxicology of chitosan 
      (CH-Au-Np) and citrate (CI-Au-Np) reduced gold nanoparticles via exploring their 
      interaction with Calf thymus DNA (Ct-DNA) utilizing various physicochemical 
      techniques. Structural characterization of these Nps was done using UV-Visible 
      Spectroscopy and Transmission Electron Microscopy (TEM). Analysis of UV-Visible 
      absorbance spectra indicates that interaction of CH-Au-Np with Ct-DNA causes 
      destabilization of DNA by inducing significant structural and conformational 
      changes in Ct-DNA in a concentration dependent manner, whereas there was 
      negligible interaction between CI-Au-Np and Ct-DNA. These observations were 
      further supported by the results of agarose gel mobility, UV-thermal melting, 
      Circular Dichroism (CD), Dynamic Light Scattering (DLS) and TEM studies. 
      Fluorescence spectral studies using acridine orange (AO) as a fluorescence probe 
      and analysis of thermodynamic parameters reveal that the interactions between 
      Ct-DNA and CH-Au-Np were mainly governed by Van der Waal interactions and 
      Hydrogen bonding. An insightful understanding of genotoxicity induced by CH-Au-Np 
      can be advantageous, as it may provide valuable anticancer approach for cytotoxic 
      drug designing.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Sonia
AU  - Sonia
AD  - Nucleic Acids Research Laboratory, Department of Chemistry, University of Delhi, 
      Delhi, India; Nano-bioconjugate Chemistry Lab, Cluster Innovation Centre, 
      University of Delhi, Delhi, India.
FAU - Komal
AU  - Komal
AD  - Nucleic Acids Research Laboratory, Department of Chemistry, University of Delhi, 
      Delhi, India; Nano-bioconjugate Chemistry Lab, Cluster Innovation Centre, 
      University of Delhi, Delhi, India.
FAU - Kukreti, Shrikant
AU  - Kukreti S
AD  - Nucleic Acids Research Laboratory, Department of Chemistry, University of Delhi, 
      Delhi, India.
FAU - Kaushik, Mahima
AU  - Kaushik M
AD  - Nano-bioconjugate Chemistry Lab, Cluster Innovation Centre, University of Delhi, 
      Delhi, India. Electronic address: mkaushik@cic.du.ac.in.
LA  - eng
PT  - Journal Article
DEP - 20180424
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 2968PHW8QP (Citric Acid)
RN  - 7440-57-5 (Gold)
RN  - 9007-49-2 (DNA)
RN  - 9012-76-4 (Chitosan)
RN  - 91080-16-9 (calf thymus DNA)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - *Chemical Phenomena
MH  - Chitosan/*chemistry
MH  - Citric Acid/*chemistry
MH  - DNA/genetics
MH  - *DNA Damage
MH  - Gold/*chemistry/*toxicity
MH  - Metal Nanoparticles/*chemistry
OTO - NOTNLM
OT  - Chitosan nanoparticle
OT  - Citrate reduced nanoparticle
OT  - DNA damaging
OT  - DNA-nanoparticle interaction
OT  - Gold nanoparticle
EDAT- 2018/04/27 06:00
MHDA- 2018/10/12 06:00
CRDT- 2018/04/27 06:00
PHST- 2018/03/13 00:00 [received]
PHST- 2018/04/22 00:00 [revised]
PHST- 2018/04/23 00:00 [accepted]
PHST- 2018/04/27 06:00 [pubmed]
PHST- 2018/10/12 06:00 [medline]
PHST- 2018/04/27 06:00 [entrez]
AID - S0141-8130(18)31220-0 [pii]
AID - 10.1016/j.ijbiomac.2018.04.115 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2018 Aug;115:801-810. doi: 10.1016/j.ijbiomac.2018.04.115. 
      Epub 2018 Apr 24.