PMID- 29700928
OWN - NLM
STAT- MEDLINE
DCOM- 20181108
LR  - 20181108
IS  - 1526-4998 (Electronic)
IS  - 1526-498X (Linking)
VI  - 74
IP  - 11
DP  - 2018 Nov
TI  - Labeling membrane receptors with lectins and evaluation of the midgut 
      histochemistry of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) populations 
      with different levels of susceptibility to formulated Bt.
PG  - 2608-2617
LID - 10.1002/ps.5051 [doi]
AB  - BACKGROUND: Studies show that insects can adapt to the toxins of Bacillus 
      thuringiensis under field and laboratory conditions through the development of 
      resistance to the bacterium and its formulations. This has been demonstrated in 
      the failure to control Tuta absoluta populations in Brazil. This study evaluated 
      membrane receptors using peroxidase-labeled lectins and the midgut histochemistry 
      of T. absoluta populations to assess susceptibility to the insecticides Bt 
      fomulations. The histochemistry analysis used Periodic Acid-Schiff for glycogen 
      and Ponceau Xylidine for total proteins. The presence of glucose/mannose and 
      N-acetylgalactosamine (GalNAc) was analyzed using specific lectins. One 
      susceptible and one tolerant population were used in the study; insects were 
      exposed to the insecticide concentrations recommended by the manufacturers. The 
      midgut was collected after an interval of 20 min and analyzed using optical 
      microscopy. RESULTS: Bt fomulation interferes with the glycogen content, whereas 
      XenTari((R)) interferes with the protein content, irrespective of the level of 
      susceptibility. High expression of GalNAc residues was observed using soybean 
      lectin labeling, indicating a direct relationship between the glycosylation 
      pattern and susceptibility to Bt fomulation in the Pelotas population. 
      CONCLUSION: The use of Bt fomulation caused greater alterations in the larval 
      intestinal histophysiology compared to the use of XenTari((R)) . (c) 2018 Society of 
      Chemical Industry.
CI  - (c) 2018 Society of Chemical Industry.
FAU - Oliveira, Andresa Cb
AU  - Oliveira AC
AD  - Department of Agronomy-Entomology, Federal Rural University of Pernambuco, 
      Recife, Brazil.
FAU - Wanderley-Teixeira, Valeria
AU  - Wanderley-Teixeira V
AD  - Department of Animal Morphology and Physiology, Federal Rural University of 
      Pernambuco, Recife, Brazil.
FAU - Silva, Cristiane Ts
AU  - Silva CT
AD  - Department of Agronomy-Entomology, Federal Rural University of Pernambuco, 
      Recife, Brazil.
FAU - Teixeira, Alvaro Ac
AU  - Teixeira AA
AD  - Department of Animal Morphology and Physiology, Federal Rural University of 
      Pernambuco, Recife, Brazil.
FAU - Siqueira, Herbert Aa
AU  - Siqueira HA
AD  - Department of Agronomy-Entomology, Federal Rural University of Pernambuco, 
      Recife, Brazil.
FAU - Cruz, Glauciane S
AU  - Cruz GS
AUID- ORCID: 0000-0001-6012-1945
AD  - Department of Agronomy-Entomology, Federal Rural University of Pernambuco, 
      Recife, Brazil.
FAU - Neto, Clovis J C Lapa
AU  - Neto CJCL
AD  - Department of Animal Morphology and Physiology, Federal Rural University of 
      Pernambuco, Recife, Brazil.
FAU - Lima, Amanda Lr
AU  - Lima AL
AD  - Departament of Biochemistry, Federal University of Pernambuco, Recife, Brazil.
FAU - Correia, Maria Ts
AU  - Correia MT
AD  - Departament of Biochemistry, Federal University of Pernambuco, Recife, Brazil.
LA  - eng
GR  - Federal Rural University of Pernambuco (UFRPE)/
GR  - Coordination for the Improvement of Higher Education Personnel (CAPES)/
GR  - Federal University of Pernambuco (UFPE)/
GR  - Federal Rural University of Pernambuco (CENAPESQ-UFRPE)/
PT  - Journal Article
DEP - 20180711
PL  - England
TA  - Pest Manag Sci
JT  - Pest management science
JID - 100898744
RN  - 0 (Insecticides)
RN  - 0 (Lectins)
RN  - IY9XDZ35W2 (Glucose)
RN  - KM15WK8O5T (Acetylgalactosamine)
RN  - PHA4727WTP (Mannose)
SB  - IM
MH  - Acetylgalactosamine/metabolism
MH  - Animals
MH  - Bacillus thuringiensis/*chemistry
MH  - *Carbohydrate Metabolism
MH  - Digestive System/anatomy & histology
MH  - Digestive System Physiological Phenomena
MH  - Glucose/metabolism
MH  - *Insecticide Resistance
MH  - Insecticides/*pharmacology
MH  - Larva/growth & development/metabolism/physiology
MH  - Lectins/*chemistry
MH  - Mannose/metabolism
MH  - Moths/growth & development/microbiology/*physiology
OTO - NOTNLM
OT  - Histopathology
OT  - N-acetylgalactosamine (GalNAc)
OT  - lectins
OT  - morphology
EDAT- 2018/04/28 06:00
MHDA- 2018/11/09 06:00
CRDT- 2018/04/28 06:00
PHST- 2017/04/10 00:00 [received]
PHST- 2018/03/21 00:00 [revised]
PHST- 2018/04/20 00:00 [accepted]
PHST- 2018/04/28 06:00 [pubmed]
PHST- 2018/11/09 06:00 [medline]
PHST- 2018/04/28 06:00 [entrez]
AID - 10.1002/ps.5051 [doi]
PST - ppublish
SO  - Pest Manag Sci. 2018 Nov;74(11):2608-2617. doi: 10.1002/ps.5051. Epub 2018 Jul 
      11.