PMID- 29701755
OWN - NLM
STAT- MEDLINE
DCOM- 20190927
LR  - 20190927
IS  - 1529-7268 (Electronic)
IS  - 0006-3363 (Linking)
VI  - 99
IP  - 4
DP  - 2018 Oct 1
TI  - Domain-functional analyses of PIWIL1 and PABPC1 indicate their synergistic roles 
      in protein translation via 3'-UTRs of meiotic mRNAs.
PG  - 773-788
LID - 10.1093/biolre/ioy100 [doi]
AB  - Translational regulation plays a central role during post-meiotic development of 
      male germ cells. Previous studies suggested that P-element induced wimpy testis 
      like 1 (PIWIL1), a PIWI-interacting RNA (piRNA) binding protein that is critical 
      for sperm development, participates in the maintenance and translational 
      regulation of post-meiotic mRNAs in haploid spermatids. However, how PIWIL1 
      regulates protein translation remains largely unclear. Using biochemical assays, 
      we show here that PIWIL1 utilizes different domains to interact with post-meiotic 
      mRNAs and Poly-A binding protein cytoplasmic 1 (PABPC1), a general protein 
      translation regulator. PIWIL1 binds 3'-untranslated regions (3'-UTRs) of several 
      spermiogenic mRNAs via its N-terminal domain, whereas its interactions with 
      PABPC1 are mediated through its N- and C-terminal domains in an RNA-dependent 
      manner. Using a heterologous cell system, we analyzed its effects on protein 
      translation via luciferase reporter assay and sucrose gradient sedimentation. It 
      was found that PIWIL1 augments protein translation with PABPC1 in the presence of 
      3'-UTRs of post-meiotic mRNAs. While both the N-terminal RNA recognition motif 
      (RRM) domain and the central linker region of PABPC1 stimulate translation, only 
      the PIWI Argonaute and Zwille (PAZ) domain of PIWIL1 positively affects 
      translation of reporter mRNAs. Interestingly, the PAZ domain was found absent 
      from polysomal fractions, in contrast to the N- and C-terminal domains of PIWIL1. 
      Taken together, the results suggest that PIWIL1 interacts with various partners 
      using different domains and participates in translational regulation partly 
      through 3'-UTRs. It will be of interest to further explore how PIWIL1 elicit its 
      versatile functions, including translational regulation of post-meiotic mRNAs 
      through intrinsic structural changes and extrinsic signals during mouse 
      spermiogenesis under more physiological settings.
FAU - Wu, Yaoyao
AU  - Wu Y
AD  - School of Life Science, University of Science and Technology of China, Hefei, 
      China.
AD  - Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of 
      Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and 
      Health, Chinese Academy of Sciences, Guangzhou, China.
FAU - Xu, Kaibiao
AU  - Xu K
AD  - Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of 
      Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and 
      Health, Chinese Academy of Sciences, Guangzhou, China.
FAU - Qi, Huayu
AU  - Qi H
AD  - Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of 
      Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and 
      Health, Chinese Academy of Sciences, Guangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Argonaute Proteins)
RN  - 0 (Piwil1 protein, mouse)
RN  - 0 (Poly(A)-Binding Protein I)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Animals
MH  - Argonaute Proteins/*chemistry/genetics/*metabolism
MH  - HEK293 Cells
MH  - Humans
MH  - Male
MH  - Meiosis/genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Poly(A)-Binding Protein I/*chemistry/genetics/*metabolism
MH  - Protein Biosynthesis
MH  - Protein Interaction Domains and Motifs
MH  - RNA, Messenger/*genetics/*metabolism
MH  - Recombinant Proteins/chemistry/genetics/metabolism
MH  - Spermatogenesis/*genetics
MH  - Testis/metabolism
EDAT- 2018/04/28 06:00
MHDA- 2019/09/29 06:00
CRDT- 2018/04/28 06:00
PHST- 2017/07/19 00:00 [received]
PHST- 2018/04/24 00:00 [accepted]
PHST- 2018/04/28 06:00 [pubmed]
PHST- 2019/09/29 06:00 [medline]
PHST- 2018/04/28 06:00 [entrez]
AID - 4985832 [pii]
AID - 10.1093/biolre/ioy100 [doi]
PST - ppublish
SO  - Biol Reprod. 2018 Oct 1;99(4):773-788. doi: 10.1093/biolre/ioy100.