PMID- 29702083
OWN - NLM
STAT- MEDLINE
DCOM- 20190506
LR  - 20190506
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1691
DP  - 2018 Jul 15
TI  - alphavbeta5 integrin mediates the effect of vitronectin on the initial stage of 
      differentiation in mouse cerebellar granule cell precursors.
PG  - 94-104
LID - S0006-8993(18)30219-1 [pii]
LID - 10.1016/j.brainres.2018.04.025 [doi]
AB  - Vitronectin (VN), one of the extracellular matrix proteins, controls the 
      maturation of cerebellar granule cells (CGCs) through the promotion of the 
      initial differentiation stage progress. However, the receptors of VN in the 
      initial differentiation stage of CGC precursors (CGCPs) have not been clarified. 
      In this study, we characterized the receptor candidates for VN in CGCPs. First, 
      we confirmed that alphavbeta3 and alphavbeta5 integrins, which are receptor candidates for VN, 
      were co-localized with VN in the developing cerebellum and primary cultured 
      CGCPs. Next, the knockdown (KD) of alphav, beta3, and beta5 integrins with small 
      interference RNA (siRNA) for each integrin reduced the ratio of Tuj1, a final 
      differentiation marker, -positive CGCPs. We further studied whether alphavbeta3 and alphavbeta5 
      integrins control the initial differentiation stage. The KD of alphav and beta5, but not 
      beta3, integrins significantly increased the ratio of transient axonal glycoprotein 
      1 (TAG1), an initial differentiation marker, -positive CGCPs, whereas the KD of 
      alphav and beta3 integrins, not beta5 integrin, stimulated the proliferation of CGCPs. 
      Overexpression of beta5 integrin stimulated the progress of the initial 
      differentiation stage as well. To confirm the interaction between alphavbeta5 integrin 
      and VN, VN was added to beta5 integrin-KD CGCPs. The promotion of the progress of 
      initial differentiation by VN was abrogated by beta5 integrin KD using small hairpin 
      RNA (shRNA). Taken together, our results indicated that alphavbeta5 integrin, as the 
      very receptor of VN, is responsible for the progress of the initial 
      differentiation stage in mouse CGCPs.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Abe, Ayaka
AU  - Abe A
AD  - Graduate School of Humanities and Sciences, Ochanomizu University, Otsuka, 
      Bunkyo-ku, Tokyo, Japan; Institute for Human Life Innovation, Ochanomizu 
      University, Otsuka, Bunkyo-ku, Tokyo, Japan.
FAU - Hashimoto, Kei
AU  - Hashimoto K
AD  - Graduate School of Humanities and Sciences, Ochanomizu University, Otsuka, 
      Bunkyo-ku, Tokyo, Japan; Institute for Human Life Innovation, Ochanomizu 
      University, Otsuka, Bunkyo-ku, Tokyo, Japan; Japan Society for the Promotion of 
      Science, Kojimachi, Chiyoda-ku, Tokyo, Japan.
FAU - Akiyama, Ayumi
AU  - Akiyama A
AD  - Graduate School of Humanities and Sciences, Ochanomizu University, Otsuka, 
      Bunkyo-ku, Tokyo, Japan.
FAU - Iida, Momoe
AU  - Iida M
AD  - Graduate School of Humanities and Sciences, Ochanomizu University, Otsuka, 
      Bunkyo-ku, Tokyo, Japan.
FAU - Ikeda, Natsumi
AU  - Ikeda N
AD  - Graduate School of Humanities and Sciences, Ochanomizu University, Otsuka, 
      Bunkyo-ku, Tokyo, Japan.
FAU - Hamano, Ayana
AU  - Hamano A
AD  - Graduate School of Humanities and Sciences, Ochanomizu University, Otsuka, 
      Bunkyo-ku, Tokyo, Japan; Institute for Human Life Innovation, Ochanomizu 
      University, Otsuka, Bunkyo-ku, Tokyo, Japan.
FAU - Watanabe, Riho
AU  - Watanabe R
AD  - Graduate School of Humanities and Sciences, Ochanomizu University, Otsuka, 
      Bunkyo-ku, Tokyo, Japan.
FAU - Hayashi, Yokichi
AU  - Hayashi Y
AD  - Department of Life Science, Asahikawa Medical University, Asahikawa, Hokkaido, 
      Japan.
FAU - Miyamoto, Yasunori
AU  - Miyamoto Y
AD  - Graduate School of Humanities and Sciences, Ochanomizu University, Otsuka, 
      Bunkyo-ku, Tokyo, Japan; Institute for Human Life Innovation, Ochanomizu 
      University, Otsuka, Bunkyo-ku, Tokyo, Japan. Electronic address: 
      miyamoto.yasunori@ocha.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180424
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Contactin 2)
RN  - 0 (Integrin alphaVbeta3)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptors, Vitronectin)
RN  - 0 (Tubulin)
RN  - 0 (Vitronectin)
RN  - 0 (beta3 tubulin, mouse)
RN  - 0 (integrin alphaVbeta5)
RN  - EC 4.2.1.11 (Phosphopyruvate Hydratase)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Animals, Newborn
MH  - Cell Differentiation/genetics
MH  - Cell Proliferation/genetics
MH  - Cells, Cultured
MH  - Cerebellum/*cytology/growth & development
MH  - Contactin 2/metabolism
MH  - Gene Expression Regulation, Developmental/*physiology
MH  - Integrin alphaVbeta3/genetics/metabolism
MH  - Ki-67 Antigen/metabolism
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Neural Stem Cells/*physiology
MH  - Organ Culture Techniques
MH  - Phosphopyruvate Hydratase/metabolism
MH  - RNA, Small Interfering/genetics/metabolism
MH  - Receptors, Vitronectin/*genetics/*metabolism
MH  - Tubulin/metabolism
MH  - Vitronectin/metabolism
OTO - NOTNLM
OT  - Cerebellar granule cell
OT  - Differentiation
OT  - Vitronectin
OT  - alphavbeta3 integrin
OT  - alphavbeta5 integrin
EDAT- 2018/04/28 06:00
MHDA- 2019/05/07 06:00
CRDT- 2018/04/28 06:00
PHST- 2017/08/25 00:00 [received]
PHST- 2018/03/29 00:00 [revised]
PHST- 2018/04/20 00:00 [accepted]
PHST- 2018/04/28 06:00 [pubmed]
PHST- 2019/05/07 06:00 [medline]
PHST- 2018/04/28 06:00 [entrez]
AID - S0006-8993(18)30219-1 [pii]
AID - 10.1016/j.brainres.2018.04.025 [doi]
PST - ppublish
SO  - Brain Res. 2018 Jul 15;1691:94-104. doi: 10.1016/j.brainres.2018.04.025. Epub 
      2018 Apr 24.