PMID- 29704805 OWN - NLM STAT- MEDLINE DCOM- 20190115 LR - 20190115 IS - 1873-6750 (Electronic) IS - 0160-4120 (Linking) VI - 116 DP - 2018 Jul TI - Advanced data mining approaches in the assessment of urinary concentrations of bisphenols, chlorophenols, parabens and benzophenones in Brazilian children and their association to DNA damage. PG - 269-277 LID - S0160-4120(18)30001-1 [pii] LID - 10.1016/j.envint.2018.04.023 [doi] AB - Human exposure to endocrine disrupting chemicals (EDCs) has received considerable attention over the last three decades. However, little is known about the influence of co-exposure to multiple EDCs on effect-biomarkers such as oxidative stress in Brazilian children. In this study, concentrations of 40 EDCs were determined in urine samples collected from 300 Brazilian children of ages 6-14 years and data were analyzed by advanced data mining techniques. Oxidative DNA damage was evaluated from the urinary concentrations of 8-hydroxy-2'-deoxyguanosine (8OHDG). Fourteen EDCs, including bisphenol A (BPA), methyl paraben (MeP), ethyl paraben (EtP), propyl paraben (PrP), 3,4-dihydroxy benzoic acid (3,4-DHB), methyl-protocatechuic acid (OH-MeP), ethyl-protocatechuic acid (OH-EtP), triclosan (TCS), triclocarban (TCC), 2-hydroxy-4-methoxybenzophenone (BP3), 2,4-dihydroxybenzophenone (BP1), bisphenol A bis(2,3-dihydroxypropyl) glycidyl ether (BADGE.2H(2)O), 2,4-dichlorophenol (2,4-DCP), and 2,5-dichlorophenol (2,5-DCP) were found in >50% of the urine samples analyzed. The highest geometric mean concentrations were found for MeP (43.1 ng/mL), PrP (3.12 ng/mL), 3,4-DHB (42.2 ng/mL), TCS (8.26 ng/mL), BP3 (3.71 ng/mL), and BP1 (4.85 ng/mL), and exposures to most of which were associated with personal care product (PCP) use. Statistically significant associations were found between urinary concentrations of 8OHDG and BPA, MeP, 3,4-DHB, OH-MeP, OH-EtP, TCS, BP3, 2,4-DCP, and 2,5-DCP. After clustering the data on the basis of i) 14 EDCs (exposure levels), ii) demography (age, gender and geographic location), and iii) 8OHDG (effect), two distinct clusters of samples were identified. 8OHDG concentration was the most critical parameter that differentiated the two clusters, followed by OH-EtP. When 8OHDG was removed from the dataset, predictability of exposure variables increased in the order of: OH-EtP > OH-MeP > 3,4-DHB > BPA > 2,4-DCP > MeP > TCS > EtP > BP1 > 2,5-DCP. Our results showed that co-exposure to OH-EtP, OH-MeP, 3,4-DHB, BPA, 2,4-DCP, MeP, TCS, EtP, BP1, and 2,5-DCP was associated with DNA damage in children. This is the first study to report exposure of Brazilian children to a wide range of EDCs and the data mining approach further strengthened our findings of chemical co-exposures and biomarkers of effect. CI - Copyright (c) 2018 Elsevier Ltd. All rights reserved. FAU - Rocha, Bruno A AU - Rocha BA AD - Laboratorio de Toxicologia e Essencialidade de Metais, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, Sao Paulo 14040-903, Brazil; Wadsworth Center, New York State Department of Health, and Department of Environmental Health Sciences, School of Public Health, State University of New York at Albany, New York 12201, United States. FAU - Asimakopoulos, Alexandros G AU - Asimakopoulos AG AD - Wadsworth Center, New York State Department of Health, and Department of Environmental Health Sciences, School of Public Health, State University of New York at Albany, New York 12201, United States; Department of Chemistry, The Norwegian University of Science and Technology (NTNU), 7491 Trondheim, Norway. FAU - Honda, Masato AU - Honda M AD - Wadsworth Center, New York State Department of Health, and Department of Environmental Health Sciences, School of Public Health, State University of New York at Albany, New York 12201, United States. FAU - da Costa, Nattane L AU - da Costa NL AD - Instituto de Informatica, Universidade Federal de Goias, Goiania, Goias 74690-900, Brazil. FAU - Barbosa, Rommel M AU - Barbosa RM AD - Instituto de Informatica, Universidade Federal de Goias, Goiania, Goias 74690-900, Brazil. FAU - Barbosa, Fernando Jr AU - Barbosa F Jr AD - Laboratorio de Toxicologia e Essencialidade de Metais, Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, Sao Paulo 14040-903, Brazil. FAU - Kannan, Kurunthachalam AU - Kannan K AD - Wadsworth Center, New York State Department of Health, and Department of Environmental Health Sciences, School of Public Health, State University of New York at Albany, New York 12201, United States; Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia. Electronic address: kurunthachalam.kannan@health.ny.gov. LA - eng GR - U2C ES026542/ES/NIEHS NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20180425 PL - Netherlands TA - Environ Int JT - Environment international JID - 7807270 RN - 0 (Benzene Derivatives) SB - IM MH - Benzene Derivatives/*urine MH - Brazil/epidemiology MH - Child MH - Computational Biology MH - *DNA Damage MH - Data Mining/*methods MH - Humans OTO - NOTNLM OT - Children OT - Data mining OT - Endocrine disrupting chemicals OT - Human co-exposure OT - Oxidative stress EDAT- 2018/04/29 06:00 MHDA- 2019/01/16 06:00 CRDT- 2018/04/29 06:00 PHST- 2018/01/01 00:00 [received] PHST- 2018/04/15 00:00 [revised] PHST- 2018/04/16 00:00 [accepted] PHST- 2018/04/29 06:00 [pubmed] PHST- 2019/01/16 06:00 [medline] PHST- 2018/04/29 06:00 [entrez] AID - S0160-4120(18)30001-1 [pii] AID - 10.1016/j.envint.2018.04.023 [doi] PST - ppublish SO - Environ Int. 2018 Jul;116:269-277. doi: 10.1016/j.envint.2018.04.023. Epub 2018 Apr 25.