PMID- 2970642
OWN - NLM
STAT- MEDLINE
DCOM- 19881006
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 85
IP  - 17
DP  - 1988 Sep
TI  - Isolation and characterization of cDNAs coding for the beta subunit of the 
      high-affinity receptor for immunoglobulin E.
PG  - 6483-7
AB  - Among receptors that bind the Fc region of immunoglobulins ("Fc receptors"), only 
      the one with high affinity for immunoglobulin E (IgE) is known to consist of more 
      than a single polypeptide. In addition to the IgE-binding alpha chain, the 
      receptor contains a single beta chain and two, disulfide-linked, gamma chains. 
      From a cDNA library of a rat mucosal mast cell tumor, from which we recently 
      cloned cDNAs coding for the alpha chain, we have now isolated cDNAs coding for 
      the beta subunit. In vitro transcription-translation of the cDNA directed the 
      synthesis of a polypeptide reactive with two distinctive anti-beta monoclonal 
      antibodies and whose molecular weight was identical to that of authentic beta 
      chains. Polyclonal antibodies to beta peptides expressed in Escherichia coli 
      reacted with intact receptors and isolated beta chains. The gene encodes a 
      protein of 243 residues with no leader sequence. A hydropathicity plot suggests 
      that the polypeptide crosses the plasma membrane four times. The epitope 
      recognized by one of the monoclonal antibodies was localized to the NH2 terminus; 
      that by the other was localized to the COOH terminus. Since those antibodies 
      react with membranes and not with intact cells, we suggest that both ends of the 
      beta subunit are cytoplasmic. RNA transfer blots at high stringency failed to 
      reveal mRNA for beta chains in a variety of cells (in particular, monocytes) that 
      do not contain the high-affinity receptor for IgE.
FAU - Kinet, J P
AU  - Kinet JP
AD  - Section on Chemical Immunology, National Institute of Arthritis and 
      Musculoskeletal and Skin Diseases, Bethesda, MD 20892.
FAU - Blank, U
AU  - Blank U
FAU - Ra, C
AU  - Ra C
FAU - White, K
AU  - White K
FAU - Metzger, H
AU  - Metzger H
FAU - Kochan, J
AU  - Kochan J
LA  - eng
SI  - GENBANK/J03845
SI  - GENBANK/M22923
SI  - GENBANK/M22924
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Receptors, IgE)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Differentiation/*genetics
MH  - Base Sequence
MH  - Basophils/immunology
MH  - Cloning, Molecular
MH  - DNA/genetics/*isolation & purification
MH  - *Genes
MH  - Immunoglobulin E/*metabolism
MH  - Leukemia, Experimental/immunology
MH  - Macromolecular Substances
MH  - Molecular Sequence Data
MH  - Rats
MH  - Receptors, IgE
PMC - PMC281997
EDAT- 1988/09/01 00:00
MHDA- 1988/09/01 00:01
PMCR- 1989/03/01
CRDT- 1988/09/01 00:00
PHST- 1988/09/01 00:00 [pubmed]
PHST- 1988/09/01 00:01 [medline]
PHST- 1988/09/01 00:00 [entrez]
PHST- 1989/03/01 00:00 [pmc-release]
AID - 10.1073/pnas.85.17.6483 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1988 Sep;85(17):6483-7. doi: 10.1073/pnas.85.17.6483.