PMID- 29707905 OWN - NLM STAT- MEDLINE DCOM- 20190107 LR - 20190107 IS - 1753-0407 (Electronic) IS - 1753-0407 (Linking) VI - 10 IP - 11 DP - 2018 Nov TI - Human herpesvirus 8 infection DNA positivity is associated with low insulin secretion: A case-control study in a sub-Saharan African population with diabetes. PG - 866-873 LID - 10.1111/1753-0407.12777 [doi] AB - BACKGROUND: Viruses have been considered potential triggers for the development of diabetes. This study assessed insulin secretion and insulin sensitivity in human herpesvirus 8 (HHV8)-infected and uninfected sub-Saharan African people with diabetes. METHODS: In all, 173 people with non-autoimmune diabetes were enrolled consecutively: 124 with type 2 diabetes mellitus (T2DM) and 49 with ketosis-prone diabetes (KPD) admitted in hyperglycemic crisis. Those with KPD were further subdivided into those with new-onset ketotic-phase KPD (n = 34) or non-ketotic phase KPD (n = 15). All participants were screened for HHV8-specific antibodies and genomic DNA. Blood samples were collected for analysis of fasting glucose, HbA1c, lipid profile, and C-peptide, with insulin resistance and secretion estimated by homeostasis model assessment. RESULTS: Among the 173 diabetic participants, 88 (50.9%) were positive for HHV8 antibodies (Ac-HHV8+), including 15 (8.7%) positive for HHV8 DNA (DNA-HHV8+). The seroprevalence of HHV8 was similar between T2DM (55.6%) and KPD (61.2%) subjects. Of those with and without ketotic-phase KPD, 35.3% and 46.7% were Ac-HHV8+, respectively. Body mass index was significantly in lower DNA-HHV8+ than DNA-HHV8- subjects. Low-density lipoprotein and total cholesterol were significantly higher, but C-peptide and homeostatic model assessment of beta-cell function (HOMA-beta) were significantly lower in DNA-HHV8+ than DNA-HHV8- participants. After excluding DNA-HHV8+ participants, triglyceride concentrations were significantly higher in Ac-HHV8+ (n = 73) than Ac-HHV8- (n = 85) subjects. In contrast, HOMA-beta was significantly higher among Ac-HHV8+ than Ac-HHV8- participants. CONCLUSIONS: In the present study, HHV8 DNA positivity was associated with low insulin secretion in this sub-Saharan African diabetes population. CI - (c) 2018 Ruijin Hospital, Shanghai Jiaotong University School of Medicine and John Wiley & Sons Australia, Ltd. FAU - Lontchi-Yimagou, Eric AU - Lontchi-Yimagou E AUID- ORCID: 0000-0003-1071-3154 AD - Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaounde 1, Yaounde, Cameroon. FAU - Legoff, Jerome AU - Legoff J AD - Universite Paris Diderot, Sorbonne Paris Cite, INSERM U941, Microbiology Laboratory, Saint-Louis Hospital, Assistance Publique-Hopitaux de Paris, Paris, France. FAU - Nguewa, Jean-Louis AU - Nguewa JL AD - INSERM, UMRS 1138, Cordeliers Research Centre, Paris, France. FAU - Boudou, Philippe AU - Boudou P AD - Unit of Hormonal Biology, Department of Biochemistry, Saint-Louis University Hospital, Assistance Publique-Hopitaux de Paris, Paris, France. FAU - Balti, Eric V AU - Balti EV AD - Diabetes Research Center, Brussels Free University - VUB, Brussels, Belgium. FAU - Noubiap, Jean J AU - Noubiap JJ AD - Department of Medicine, Groote Schuur Hospital and Universty of Cape Town, Cape Town, South Africa. FAU - Kamwa, Vicky AU - Kamwa V AD - National Obesity Centre, Yaounde Central Hospital, Yaounde, Cameroon. FAU - Atogho-Tiedeu, Barbara AU - Atogho-Tiedeu B AD - Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaounde 1, Yaounde, Cameroon. FAU - Azabji-Kenfack, Marcel AU - Azabji-Kenfack M AD - Department of Physiological Sciences and Biochemistry, Faculty of Medicine and Biomedical Sciences, University of Yaounde 1, Yaounde, Cameroon. FAU - Djahmeni, Eric N AU - Djahmeni EN AD - National Obesity Centre, Yaounde Central Hospital, Yaounde, Cameroon. FAU - Etoa, Martine AU - Etoa M AD - National Obesity Centre, Yaounde Central Hospital, Yaounde, Cameroon. FAU - Lemdjo, Gaelle AU - Lemdjo G AD - National Obesity Centre, Yaounde Central Hospital, Yaounde, Cameroon. FAU - Balla, Vanessa AU - Balla V AD - National Obesity Centre, Yaounde Central Hospital, Yaounde, Cameroon. FAU - Dehayem, Mesmin Y AU - Dehayem MY AD - National Obesity Centre, Yaounde Central Hospital, Yaounde, Cameroon. FAU - Foufelle, Fabienne AU - Foufelle F AD - INSERM, UMRS 1138, Cordeliers Research Centre, Paris, France. FAU - Mbanya, Jean-Claude AU - Mbanya JC AD - Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaounde 1, Yaounde, Cameroon. AD - National Obesity Centre, Yaounde Central Hospital, Yaounde, Cameroon. AD - Department of Internal Medicine, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon. FAU - Gautier, Jean-Francois AU - Gautier JF AD - INSERM, UMRS 1138, Cordeliers Research Centre, Paris, France. FAU - Sobngwi, Eugene AU - Sobngwi E AD - Laboratory for Molecular Medicine and Metabolism, Biotechnology Center, University of Yaounde 1, Yaounde, Cameroon. AD - National Obesity Centre, Yaounde Central Hospital, Yaounde, Cameroon. AD - Department of Internal Medicine, Faculty of Medicine and Biomedical Sciences, University of Yaounde I, Yaounde, Cameroon. LA - eng PT - Journal Article DEP - 20180528 PL - Australia TA - J Diabetes JT - Journal of diabetes JID - 101504326 RN - 0 (Biomarkers) RN - 0 (DNA, Viral) RN - 0 (Insulin) SB - IM MH - Adult MH - Biomarkers/blood MH - Cameroon/epidemiology MH - Case-Control Studies MH - DNA, Viral/*genetics MH - Diabetes Mellitus/blood/diagnosis/epidemiology/*virology MH - Female MH - Herpesviridae Infections/blood/diagnosis/epidemiology/*virology MH - Herpesvirus 8, Human/*genetics/pathogenicity MH - Host-Pathogen Interactions MH - Humans MH - Insulin/*blood MH - Male MH - Middle Aged MH - Risk Factors MH - Secretory Pathway MH - Viral Load OTO - NOTNLM OT - 2型糖尿病 OT - 8型人类疱疹病毒 OT - human herpesvirus type 8 OT - insulin resistance OT - insulin secretion OT - ketosis-pronediabetes OT - type 2 diabetes OT - 胰岛素分泌 OT - 胰岛素抵抗 OT - 酮症倾向性糖尿病 EDAT- 2018/05/01 06:00 MHDA- 2019/01/08 06:00 CRDT- 2018/05/01 06:00 PHST- 2018/02/09 00:00 [received] PHST- 2018/04/07 00:00 [revised] PHST- 2018/04/22 00:00 [accepted] PHST- 2018/05/01 06:00 [pubmed] PHST- 2019/01/08 06:00 [medline] PHST- 2018/05/01 06:00 [entrez] AID - 10.1111/1753-0407.12777 [doi] PST - ppublish SO - J Diabetes. 2018 Nov;10(11):866-873. doi: 10.1111/1753-0407.12777. Epub 2018 May 28.