PMID- 29708212 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220318 IS - 2509-9280 (Electronic) IS - 2509-9280 (Linking) VI - 2 IP - 1 DP - 2018 TI - Myocardial tissue characterization by combining late gadolinium enhancement imaging and percent edema mapping: a novel T2 map-based MRI method in canine myocardial infarction. PG - 6 LID - 10.1186/s41747-018-0037-6 [doi] LID - 6 AB - BACKGROUND: Assessing the extent of ischemic and reperfusion-associated myocardial injuries remains challenging with current magnetic resonance imaging (MRI) techniques. Our aim was to develop a tissue characterization mapping (TCM) technique by combining late gadolinium enhancement (LGE) with our novel percent edema mapping (PEM) approach to enable the classification of tissue represented by MRI voxels as healthy, myocardial edema (ME), necrosis, myocardial hemorrhage (MH), or scar. METHODS: Six dogs underwent closed-chest myocardial infarct (MI) generation. Serial MRI scans were performed post-MI on days 3, 4, 6, 14, and 56, including T2 mapping and LGE. Dogs were sacrificed on day 4 (n = 4, acute MI) or day 56 (n = 2, chronic MI). TCMs were generated based on a voxel classification algorithm taking into account signal intensity from LGE and T2-based estimation of ME. TCM-based MI and MH were validated with post mortem triphenyl tetrazolium chloride (TTC) staining. Pearson's correlation and Bland-Altman analyses were performed. RESULTS: The MI, ME, and MH measured by TCM were 13.4% [25(th)-75(th) percentile 1.6-28.8], 28.1% [2.1-37.5] and 4.3% [1.0-11.3], respectively. TCM measured higher MH and MI compared to TTC (p = 0.0033 and p = 0.0007, respectively). MH size was linearly correlated with MI size by both MRI (r = 0.9528, p < 0.0001) and TTC (r = 0.9625, p < 0.0001). MH quantification demonstrated good agreement between TCM and TTC (r = 0.8766, p < 0.0001, 2.4% overestimation by TCM). A similar correlation was observed for MI size (r = 0.9429, p < 0.0001, 6.1% overestimation by TCM). CONCLUSIONS: Preliminary results suggest that the TCM method is feasible for the in vivo localization and quantification of various MI-related tissue components. FAU - Suranyi, Pal AU - Suranyi P AD - 1Division of Cardiovascular Imaging, Department of Radiology and Radiological Science, Medical University of South Carolina, 25 Courtenay Dr, Charleston, SC 29425 USA. ISNI: 0000 0001 2189 3475. GRID: grid.259828.c FAU - Elgavish, Gabriel A AU - Elgavish GA AD - 2Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, MCLM 556, Birmingham, AL 35294-0005 USA. ISNI: 0000000106344187. GRID: grid.265892.2 FAU - Schoepf, U Joseph AU - Schoepf UJ AD - 1Division of Cardiovascular Imaging, Department of Radiology and Radiological Science, Medical University of South Carolina, 25 Courtenay Dr, Charleston, SC 29425 USA. ISNI: 0000 0001 2189 3475. GRID: grid.259828.c FAU - Ruzsics, Balazs AU - Ruzsics B AD - 3Department of Cardiology, Royal Liverpool and Broadgreen University Hospital, Thomas Dr, Liverpool, L14 3LB UK. ISNI: 0000 0004 0421 1585. GRID: grid.269741.f FAU - Kiss, Pal AU - Kiss P AD - 2Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, MCLM 556, Birmingham, AL 35294-0005 USA. ISNI: 0000000106344187. GRID: grid.265892.2 FAU - van Assen, Marly AU - van Assen M AD - 1Division of Cardiovascular Imaging, Department of Radiology and Radiological Science, Medical University of South Carolina, 25 Courtenay Dr, Charleston, SC 29425 USA. ISNI: 0000 0001 2189 3475. GRID: grid.259828.c AD - 4University of Groningen, University Medical Center Groningen, Center for Medical Imaging - North East Netherlands, Hanzeplein 1, Groningen, 9713GZ The Netherlands. ISNI: 0000 0000 9558 4598. GRID: grid.4494.d FAU - Jacobs, Brian E AU - Jacobs BE AD - 1Division of Cardiovascular Imaging, Department of Radiology and Radiological Science, Medical University of South Carolina, 25 Courtenay Dr, Charleston, SC 29425 USA. ISNI: 0000 0001 2189 3475. GRID: grid.259828.c FAU - Brott, Brigitta C AU - Brott BC AD - 5Department of Medicine, Division of Cardiovascular Disease, University of Alabama at Birmingham, FOT 907, Birmingham, AL 35294-3407 USA. ISNI: 0000000106344187. GRID: grid.265892.2 FAU - Elgavish, Ada AU - Elgavish A AD - 6Department of Medicine, Division of Clinical Immunology and Rheumatology, University of Alabama at Birmingham, MCLM 556, Birmingham, AL 35294-0005 USA. ISNI: 0000000106344187. GRID: grid.265892.2 FAU - Varga-Szemes, Akos AU - Varga-Szemes A AUID- ORCID: 0000-0002-2781-7462 AD - 1Division of Cardiovascular Imaging, Department of Radiology and Radiological Science, Medical University of South Carolina, 25 Courtenay Dr, Charleston, SC 29425 USA. ISNI: 0000 0001 2189 3475. GRID: grid.259828.c AD - 2Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, MCLM 556, Birmingham, AL 35294-0005 USA. ISNI: 0000000106344187. GRID: grid.265892.2 LA - eng GR - R41 HL080886/HL/NHLBI NIH HHS/United States GR - R41 HL084795/HL/NHLBI NIH HHS/United States PT - Journal Article DEP - 20180316 PL - England TA - Eur Radiol Exp JT - European radiology experimental JID - 101721752 PMC - PMC5909369 OTO - NOTNLM OT - Magnetic resonance imaging OT - Myocardial edema OT - Myocardial hemorrhage OT - Myocardial infarction OT - Tissue characterization COIS- The study protocol was approved by the Institutional Animal Care and Use Committee at the University of Alabama at Birmingham and complied with the Guide for the Care and Use of Laboratory Animals (National Institutes of Health).Not applicable.Dr. Schoepf receives institutional research support from Astellas, Bayer Healthcare, General Electric Healthcare, and Siemens Healthineers. Dr. Varga-Szemes receives institutional research support from Siemens Healthineers. Dr. Schoepf has received consulting or speaking fees from Bayer, Guerbet, HeartFlow, and Siemens Healthineers. The other authors have no competing interests to disclose.Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. EDAT- 2018/05/01 06:00 MHDA- 2018/05/01 06:01 PMCR- 2018/03/16 CRDT- 2018/05/01 06:00 PHST- 2017/12/15 00:00 [received] PHST- 2018/01/23 00:00 [accepted] PHST- 2018/05/01 06:00 [entrez] PHST- 2018/05/01 06:00 [pubmed] PHST- 2018/05/01 06:01 [medline] PHST- 2018/03/16 00:00 [pmc-release] AID - 37 [pii] AID - 10.1186/s41747-018-0037-6 [doi] PST - ppublish SO - Eur Radiol Exp. 2018;2(1):6. doi: 10.1186/s41747-018-0037-6. Epub 2018 Mar 16.