PMID- 29708291 OWN - NLM STAT- MEDLINE DCOM- 20180912 LR - 20210109 IS - 1740-0929 (Electronic) IS - 1344-3941 (Print) IS - 1344-3941 (Linking) VI - 89 IP - 7 DP - 2018 Jul TI - Phenotypic and functional analysis of bovine peripheral blood dendritic cells before parturition by a novel purification method. PG - 1011-1019 LID - 10.1111/asj.13014 [doi] AB - Dendritic cells (DCs) are specialized antigen presenting cells specializing in antigen uptake and processing, and play an important role in the innate and adaptive immune response. A subset of bovine peripheral blood DCs was identified as CD172a(+) /CD11c(+) /MHC (major histocompatibility complex) class II(+) cells. Although DCs are identified at 0.1%-0.7% of peripheral blood mononuclear cells (PBMC), the phenotype and function of DCs remain poorly understood with regard to maintaining tolerance during the pregnancy. All cattle used in this study were 1 month before parturition. We have established a novel method for the purification of DCs from PBMC using magnetic-activated cell sorting, and purified the CD172a(+) /CD11c(+) DCs, with high expression of MHC class II and CD40, at 84.8% purity. There were individual differences in the expressions of CD205 and co-stimulatory molecules CD80 and CD86 on DCs. There were positive correlations between expression of cytokine and co-stimulatory molecules in DCs, and the DCs maintained their immune tolerance, evidenced by their low expressions of the co-stimulatory molecules and cytokine production. These results suggest that before parturition a half of DCs may be immature and tend to maintain tolerance based on the low cytokine production, and the other DCs with high co-stimulatory molecules may already have the ability of modulating the T-cell linage. CI - (c) 2018 The Authors. Animal Science Journal published by John Wiley & Sons Australia, Ltd on behalf of Japanese Society of Animal Science. FAU - Zhuang, Tao AU - Zhuang T AUID- ORCID: 0000-0003-1475-7138 AD - Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan. AD - International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan. FAU - Urakawa, Megumi AU - Urakawa M AD - Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan. AD - International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan. FAU - Sato, Hidetoshi AU - Sato H AD - Miyagi Prefecture Animal Industry Experiment Station, Iwadeyama, Miyagi, Japan. FAU - Sato, Yuko AU - Sato Y AD - Miyagi Prefecture Animal Industry Experiment Station, Iwadeyama, Miyagi, Japan. FAU - Taguchi, Teruaki AU - Taguchi T AD - Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan. AD - International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan. FAU - Umino, Tsuyoshi AU - Umino T AD - Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan. AD - International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan. FAU - Katto, Shiro AU - Katto S AD - Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan. AD - International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan. FAU - Tanaka, Koutaro AU - Tanaka K AD - Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan. AD - International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan. FAU - Yoshimura, Kozue AU - Yoshimura K AD - Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan. AD - International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan. FAU - Takada, Naokazu AU - Takada N AD - Miyagi Prefecture Animal Industry Experiment Station, Iwadeyama, Miyagi, Japan. FAU - Kobayashi, Hiroko AU - Kobayashi H AD - Miyagi Prefecture Animal Industry Experiment Station, Iwadeyama, Miyagi, Japan. FAU - Ito, Megumi AU - Ito M AD - Miyagi Prefecture Animal Industry Experiment Station, Iwadeyama, Miyagi, Japan. FAU - Rose, Michael T AU - Rose MT AD - Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Cardiganshire, UK. FAU - Kiku, Yoshio AU - Kiku Y AD - Hokkaido Research Station, National Institute of Animal Health, NARO, Sapporo, Hokkaido, Japan. FAU - Nagasawa, Yuya AU - Nagasawa Y AUID- ORCID: 0000-0003-1392-6454 AD - Hokkaido Research Station, National Institute of Animal Health, NARO, Sapporo, Hokkaido, Japan. FAU - Kitazawa, Haruki AU - Kitazawa H AD - Food and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan. FAU - Watanabe, Kouichi AU - Watanabe K AD - Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan. AD - International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan. FAU - Nochi, Tomonori AU - Nochi T AD - Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan. AD - International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan. FAU - Hayashi, Tomohito AU - Hayashi T AD - Hokkaido Research Station, National Institute of Animal Health, NARO, Sapporo, Hokkaido, Japan. FAU - Aso, Hisashi AU - Aso H AD - Cellular Biology Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan. AD - International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan. LA - eng PT - Journal Article DEP - 20180430 PL - Australia TA - Anim Sci J JT - Animal science journal = Nihon chikusan Gakkaiho JID - 100956805 RN - 0 (Cytokines) RN - 0 (Inducible T-Cell Co-Stimulator Protein) SB - IM MH - Adaptive Immunity/immunology MH - Animals MH - Antigen Presentation/immunology MH - Cattle/*blood MH - Cell Separation/*methods MH - Cytokines/metabolism MH - Dendritic Cells/*immunology MH - Female MH - Humans MH - Immune Tolerance/*immunology MH - Immunity, Innate/immunology MH - Inducible T-Cell Co-Stimulator Protein MH - Leukocytes, Mononuclear MH - Parturition/*immunology MH - Phenotype MH - Pregnancy PMC - PMC6055732 OTO - NOTNLM OT - cattle OT - cytokine OT - dendritic cell OT - phenotype OT - positive-selection EDAT- 2018/05/01 06:00 MHDA- 2018/09/13 06:00 PMCR- 2018/07/23 CRDT- 2018/05/01 06:00 PHST- 2017/12/26 00:00 [received] PHST- 2018/01/31 00:00 [accepted] PHST- 2018/05/01 06:00 [pubmed] PHST- 2018/09/13 06:00 [medline] PHST- 2018/05/01 06:00 [entrez] PHST- 2018/07/23 00:00 [pmc-release] AID - ASJ13014 [pii] AID - 10.1111/asj.13014 [doi] PST - ppublish SO - Anim Sci J. 2018 Jul;89(7):1011-1019. doi: 10.1111/asj.13014. Epub 2018 Apr 30.