PMID- 29708864
OWN - NLM
STAT- MEDLINE
DCOM- 20181002
LR  - 20181004
IS  - 1557-8976 (Electronic)
IS  - 0882-8245 (Linking)
VI  - 31
IP  - 5
DP  - 2018 Jun
TI  - First-Time Detection of Porcine Reproductive and Respiratory Syndrome Virus and 
      Porcine Circovirus 2 in an Albanian Farrow-to-Finish Herd.
PG  - 397-403
LID - 10.1089/vim.2017.0166 [doi]
AB  - The purpose of this case report is to describe for the first time concurrent 
      porcine reproductive and respiratory syndrome virus (PRRSV) and porcine 
      circovirus 2 (PCV-2) infections in a commercial farrow-to-finish pig farm in 
      Albania, as well as the phylogenetical analysis of isolated PRRSV strain. The 
      present study reports on a farrow-to-finish commercial pig farm, located in South 
      Albania. In a percentage of about 40% of weaners in each batch (60-70 piglets per 
      batch), clinical signs, including fever, severe respiratory signs, wasting, 
      jaundice, rough hairy coat, palpable inguinal lymphadenopathy, and high mortality 
      rate, were performed. The clinical signs of sows included sporadic premature 
      farrowings (22%), with increased number of stillbirth (3.3%) and weak piglets 
      (4.1%) based on the record system of the farm. Blood samples were obtained from 8 
      sows (4 lactating and 4 dry-period sows), 25 piglets of 5 different batches (5 at 
      15-20 days, 5 at 40 days, 5 at 50 days, 5 of 60 days, and 5 of 70 days), and 5 
      finishers of 130-150 days of age. Moreover, tissue samples were collected from 
      five weaners at 20-70 days of age. Histopathological examination of lung and 
      lymph node sections revealed findings compatible with PRRSV and PCV-2 infection. 
      Pigs between 15 and 130-140 days of age were positive for type 1 (European) PRRSV 
      and pigs between 50 and 130-140 days of age were positive for PCV-2. Blood serum 
      samples were tested by real-time polymerase chain reaction (PCR) for PCV-2 and 
      one real-time reverse transcription-PCR-positive sample was selected for 
      subsequent complete ORF5 (Gp5) gene sequencing. The results of this case report 
      confirm the detection of PRRSV and PCV-2 concurrent infection in an Albanian 
      farrow-to-finish pig farm. The full-length ORF5 sequence of the detected PRRSV 
      strain (named "Mursi/AL/15") was successfully determined, revealing high 
      nucleotide identity with other type 1 European isolates.
FAU - Papatsiros, Vasileios G
AU  - Papatsiros VG
AD  - 1 Clinic of Medicine, Faculty of Veterinary Medicine, University of Thessaly , 
      Karditsa, Greece .
FAU - Chaintoutis, Serafeim C
AU  - Chaintoutis SC
AD  - 2 Diagnostic Laboratory, School of Veterinary Medicine, Faculty of Health 
      Sciences, Aristotle University of Thessaloniki , Thessaloniki, Greece .
FAU - Psalla, Dimitra
AU  - Psalla D
AD  - 3 Laboratory of Pathology, School of Veterinary Medicine, Faculty of Health 
      Sciences, Aristotle University of Thessaloniki , Thessaloniki, Greece .
FAU - Maragkakis, Georgios G
AU  - Maragkakis GG
AD  - 1 Clinic of Medicine, Faculty of Veterinary Medicine, University of Thessaly , 
      Karditsa, Greece .
FAU - Christodoulopoulos, Georgios
AU  - Christodoulopoulos G
AD  - 1 Clinic of Medicine, Faculty of Veterinary Medicine, University of Thessaly , 
      Karditsa, Greece .
FAU - Dovas, Chrysostomos I
AU  - Dovas CI
AD  - 2 Diagnostic Laboratory, School of Veterinary Medicine, Faculty of Health 
      Sciences, Aristotle University of Thessaloniki , Thessaloniki, Greece .
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20180430
PL  - United States
TA  - Viral Immunol
JT  - Viral immunology
JID - 8801552
SB  - IM
MH  - Albania
MH  - Animals
MH  - Circoviridae Infections/pathology/*veterinary/virology
MH  - Circovirus/*isolation & purification
MH  - Histocytochemistry
MH  - Lung/pathology
MH  - Lymph Nodes/pathology
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/pathology/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/classification/genetics/*isolation & purification
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Sequence Homology
MH  - Serum/virology
MH  - Swine
OTO - NOTNLM
OT  - Albania
OT  - PCV-2
OT  - PRRSV
OT  - infection
OT  - pig
EDAT- 2018/05/01 06:00
MHDA- 2018/10/03 06:00
CRDT- 2018/05/01 06:00
PHST- 2018/05/01 06:00 [pubmed]
PHST- 2018/10/03 06:00 [medline]
PHST- 2018/05/01 06:00 [entrez]
AID - 10.1089/vim.2017.0166 [doi]
PST - ppublish
SO  - Viral Immunol. 2018 Jun;31(5):397-403. doi: 10.1089/vim.2017.0166. Epub 2018 Apr 
      30.