PMID- 29710549
OWN - NLM
STAT- MEDLINE
DCOM- 20181002
LR  - 20210617
IS  - 1950-6007 (Electronic)
IS  - 0753-3322 (Linking)
VI  - 102
DP  - 2018 Jun
TI  - Human cytomegalovirus infection-induced autophagy was associated with the 
      biological behavioral changes of human umbilical vein endothelial cell (HUVEC).
PG  - 938-946
LID - S0753-3322(17)36995-0 [pii]
LID - 10.1016/j.biopha.2018.03.156 [doi]
AB  - In this study, we investigated the enacted role of autophagy in biological 
      behavioral changes of human umbilical vein endothelial cell (HUVEC) infected with 
      human cytomegalovirus (HCMV). It was found that in HCMV-infected cells, the 
      number of punctuate structures increased from 24 h to 48 h p.i. while decreased 
      at 60 h p.i. by GFP-LC3 tranfecting, Monodansylcadaverine (MDC), acridine orange 
      (AO) staining, which suggested that autophagy was induced at early stage, but it 
      was inhibited at later stage in responding to HCMV infection. By real-time qPCR 
      assay, it was revealed that mTOR autophagy pathway inhibitor rapamycin could 
      promote HCMV proliferation in HUVEC cells. After the treatment of autophagy 
      promoting agent, autophagy inhibitor, and mTOR signaling pathway inhibitor in 
      HCMV-infected HUVECs showed similar results to the above findings by Western 
      Blotting assay. Moreover, invasion assay showed that HCMV infection promoted 
      HUVEC cell migration, and ELISA assay showed that HCMV infection increased the 
      expression of adhesion molecules of HUVEC cells. In conclusion, HCMV infection 
      induced autophagy in a time-dependent manner in HUVECs with the involvement of 
      mTOR signaling pathway. The autophagy of HUVEC may enhance its cell invasiveness 
      and the expression of ICAM-1 and VCAM-1. Thus, HCMV infection-induced autophagy 
      was associated with the biological behavioral changes of HUVECs.
CI  - Copyright (c) 2018 Elsevier Masson SAS. All rights reserved.
FAU - Zhao, Jun
AU  - Zhao J
AD  - Department of Pharmacology, Anhui Medical University, Hefei, China; Department of 
      Microbiology, Anhui Medical University, Hefei, China; Wuhu Overseas Students 
      Pioneer Park, Wuhu, China.
FAU - Zhong, Feng
AU  - Zhong F
AD  - Department of Microbiology, Anhui Medical University, Hefei, China; Department of 
      Clinical Laboratory, The Second Affiliated Hospital of Wannan Medical College, 
      Wuhu, China.
FAU - Yu, Haiyang
AU  - Yu H
AD  - Department of Microbiology, Anhui Medical University, Hefei, China.
FAU - Chen, Zhiwu
AU  - Chen Z
AD  - Department of Pharmacology, Anhui Medical University, Hefei, China. Electronic 
      address: chpharmzw@163.com.
FAU - Wang, Mingli
AU  - Wang M
AD  - Department of Microbiology, Anhui Medical University, Hefei, China; Wuhu Overseas 
      Students Pioneer Park, Wuhu, China. Electronic address: microbio@ahmu.edu.cn.
FAU - Chen, Jason
AU  - Chen J
AD  - Department of Microbiology, Anhui Medical University, Hefei, China; Department of 
      Pathology & Cell Biology, Columbia University, New York, NY, USA. Electronic 
      address: jc28@cumc.columbia.edu.
LA  - eng
PT  - Journal Article
DEP - 20180405
PL  - France
TA  - Biomed Pharmacother
JT  - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
JID - 8213295
RN  - 0 (DNA, Viral)
RN  - 0 (MAP1LC3A protein, human)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
SB  - IM
MH  - *Autophagy
MH  - Cell Movement
MH  - Cytomegalovirus/*physiology
MH  - Cytomegalovirus Infections/*pathology/*virology
MH  - DNA, Viral/metabolism
MH  - Fluorescent Antibody Technique
MH  - Human Umbilical Vein Endothelial Cells/*pathology/*virology
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Microtubule-Associated Proteins/metabolism
MH  - Ribosomal Protein S6 Kinases, 70-kDa/metabolism
MH  - Vascular Cell Adhesion Molecule-1/metabolism
MH  - Virus Replication
OTO - NOTNLM
OT  - Autophagy
OT  - Biological behavioral change
OT  - Human cytomegalovirus (HCMV)
OT  - Human umbilical vein endothelial cell (HUVEC)
OT  - Mammalian target of rapamycin (mTOR)
EDAT- 2018/05/02 06:00
MHDA- 2018/10/03 06:00
CRDT- 2018/05/02 06:00
PHST- 2017/12/20 00:00 [received]
PHST- 2018/03/23 00:00 [revised]
PHST- 2018/03/26 00:00 [accepted]
PHST- 2018/05/02 06:00 [pubmed]
PHST- 2018/10/03 06:00 [medline]
PHST- 2018/05/02 06:00 [entrez]
AID - S0753-3322(17)36995-0 [pii]
AID - 10.1016/j.biopha.2018.03.156 [doi]
PST - ppublish
SO  - Biomed Pharmacother. 2018 Jun;102:938-946. doi: 10.1016/j.biopha.2018.03.156. 
      Epub 2018 Apr 5.