PMID- 29715536
OWN - NLM
STAT- MEDLINE
DCOM- 20190107
LR  - 20190816
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 120
DP  - 2018 Jul
TI  - Evaluation of Caco-2 cells response to Listeria monocytogenes virulence factors 
      by RT-PCR.
PG  - 79-84
LID - S0882-4010(18)30507-2 [pii]
LID - 10.1016/j.micpath.2018.04.059 [doi]
AB  - Listeria monocytogenes expresses various virulence factors enabling the invasion 
      and multiplying in host cells, and together induces cytokines transcription. In 
      order to explore the relationship between virulence factors of L. monocytogenes 
      wild-type EGD-e and cellular response in human colonic epithelial cell 
      line(Caco-2), we constructed mutant strains with in-frame deletions of critical 
      virulence genes of inlA, inlB, hly, actA and virulence regulatory factor prfA 
      from EGD-e, respectively. Compared with EGD-e, mutant strains showed 
      significantly decreased invasion and apoptosis in Caco-2 cells. However, mutant 
      strains were capable to evoke cytokines transcription of interleukin-8 (IL-8), 
      mononuclear chemoattractant protein-1 (MCP-1), tumor necrosis factor-a (TNF-a), 
      interleukin-1beta (IL-1beta), interleukin-6 (IL-6) and CXCL-2 production in 
      Caco-2 cells. Interestingly, EGD-e Deltahly-infected Caco-2 cells showed a 
      significant decrease of IL-6, IL-8 and MCP-1 transcription compared with EGD-e at 
      1 h post-infection. Simultaneously, EGD-e DeltainlB-infected cells showed a decrease 
      in IL-6 transcription, while EGD-e DeltaactA-infected cells reflected a decrease in 
      MCP-1 transcription. Virulence genes play a role in inflammatory transcription, 
      but the interaction between pathogenic bacteria and the host cells predominates 
      in inflammatory transcription. Overall, the data showed cellular response of 
      Caco-2 cells infected with EGD-e mutant strains.
CI  - Copyright (c) 2018. Published by Elsevier Ltd.
FAU - Xie, Manman
AU  - Xie M
AD  - School of Medical Instrument and Food Engineering, University of Shanghai for 
      Science and Technology, Shanghai 200093, PR China.
FAU - Ding, Chengchao
AU  - Ding C
AD  - School of Medical Instrument and Food Engineering, University of Shanghai for 
      Science and Technology, Shanghai 200093, PR China.
FAU - Guo, Liang
AU  - Guo L
AD  - School of Medical Instrument and Food Engineering, University of Shanghai for 
      Science and Technology, Shanghai 200093, PR China.
FAU - Chen, Guowei
AU  - Chen G
AD  - School of Medical Instrument and Food Engineering, University of Shanghai for 
      Science and Technology, Shanghai 200093, PR China.
FAU - Zeng, Haijuan
AU  - Zeng H
AD  - School of Medical Instrument and Food Engineering, University of Shanghai for 
      Science and Technology, Shanghai 200093, PR China.
FAU - Liu, Qing
AU  - Liu Q
AD  - School of Medical Instrument and Food Engineering, University of Shanghai for 
      Science and Technology, Shanghai 200093, PR China; Laboratory for Marine 
      Fisheries Science and Food Production Processes, Qingdao National Laboratory for 
      Marine Science and Technology, Shandong 266071, PR China. Electronic address: 
      liuq@usst.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20180430
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (Bacterial Proteins)
RN  - 0 (Bacterial Toxins)
RN  - 0 (CCL2 protein, human)
RN  - 0 (CXCL2 protein, human)
RN  - 0 (CXCL8 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL2)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (Membrane Proteins)
RN  - 0 (Peptide Termination Factors)
RN  - 0 (PrfA protein, Listeria monocytogenes)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Virulence Factors)
RN  - 0 (inlB protein, Listeria monocytogenes)
RN  - 139948-71-3 (internalin protein, Bacteria)
RN  - 144430-05-7 (actA protein, Listeria monocytogenes)
SB  - IM
MH  - Apoptosis/drug effects
MH  - Bacterial Proteins/adverse effects
MH  - Bacterial Toxins/adverse effects
MH  - Caco-2 Cells/*drug effects/immunology/*metabolism
MH  - Chemokine CCL2/metabolism
MH  - Chemokine CXCL2/metabolism
MH  - Cytokines/metabolism
MH  - Host-Pathogen Interactions/immunology/physiology
MH  - Humans
MH  - Interleukin-1beta/metabolism
MH  - Interleukin-6/metabolism
MH  - Interleukin-8/metabolism
MH  - Listeria monocytogenes/*metabolism/pathogenicity
MH  - Membrane Proteins/adverse effects
MH  - Peptide Termination Factors/adverse effects
MH  - Reverse Transcriptase Polymerase Chain Reaction/*methods
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Virulence/genetics
MH  - Virulence Factors/*adverse effects/genetics
OTO - NOTNLM
OT  - Caco-2
OT  - Inflammatory response
OT  - Listeria monocytogenes
OT  - Virulence factors
EDAT- 2018/05/02 06:00
MHDA- 2019/01/08 06:00
CRDT- 2018/05/02 06:00
PHST- 2018/03/20 00:00 [received]
PHST- 2018/04/16 00:00 [revised]
PHST- 2018/04/27 00:00 [accepted]
PHST- 2018/05/02 06:00 [pubmed]
PHST- 2019/01/08 06:00 [medline]
PHST- 2018/05/02 06:00 [entrez]
AID - S0882-4010(18)30507-2 [pii]
AID - 10.1016/j.micpath.2018.04.059 [doi]
PST - ppublish
SO  - Microb Pathog. 2018 Jul;120:79-84. doi: 10.1016/j.micpath.2018.04.059. Epub 2018 
      Apr 30.