PMID- 29717364
OWN - NLM
STAT- MEDLINE
DCOM- 20180828
LR  - 20180828
IS  - 1432-0584 (Electronic)
IS  - 0939-5555 (Linking)
VI  - 97
IP  - 9
DP  - 2018 Sep
TI  - Acute promyelocytic leukemia cell adhesion to vascular endothelium is reduced by 
      heparins.
PG  - 1555-1562
LID - 10.1007/s00277-018-3343-4 [doi]
AB  - Adhesion of acute promyelocytic leukemia (APL) cells to endothelial cells (EC) is 
      among the mechanisms of the APL-associated coagulopathy, responsible for early 
      hemorrhagic deaths in affected patients. We compared the effects of dalteparin 
      and enoxaparin, two low-molecular-weight heparins (LMWH), and unfractionated 
      heparin (UFH), on APL NB4 adhesion to micro- (HMEC-1) and macro-vascular EC 
      (HUVEC), in resting and interleukin-1beta (IL-1beta)-stimulated conditions. The heparin 
      effect on EC adhesion molecule (ICAM-1, VCAM-1, E-selectin) expression was also 
      assessed. In HMEC-1, dalteparin inhibited IL1beta-induced NB4 adhesion by 80%, 
      enoxaparin by 52%, and UFH by 44%. Similar results were obtained in HUVEC. This 
      was associated with a significant decrease of VCAM-1 and ICAM-1 expression. In 
      conclusion, we show that LMWH significantly counteract APL cell adhesion to the 
      vessel wall, by modulating EC adhesion molecule expression. This property of 
      heparins may represent one approach for hampering excess clotting activation and 
      microthrombi deposition in APL.
FAU - Vignoli, Alfonso
AU  - Vignoli A
AD  - Department of Immunohematology and Transfusion Medicine & Hemostasis and 
      Thrombosis Center, Hospital Papa Giovanni XXIII, Piazza O.M.S., 1, 24127, 
      Bergamo, Italy.
FAU - Marchetti, Marina
AU  - Marchetti M
AD  - Department of Immunohematology and Transfusion Medicine & Hemostasis and 
      Thrombosis Center, Hospital Papa Giovanni XXIII, Piazza O.M.S., 1, 24127, 
      Bergamo, Italy.
FAU - Falanga, Anna
AU  - Falanga A
AUID- ORCID: 0000-0002-5007-3457
AD  - Department of Immunohematology and Transfusion Medicine & Hemostasis and 
      Thrombosis Center, Hospital Papa Giovanni XXIII, Piazza O.M.S., 1, 24127, 
      Bergamo, Italy. annafalanga@yahoo.com.
LA  - eng
GR  - IG14505/Associazione Italiana per la Ricerca sul Cancro (A.I.R.C.)/
GR  - "5x1000" n. 12237/Associazione Italiana per la Ricerca sul Cancro (A.I.R.C.)/
PT  - Journal Article
DEP - 20180501
PL  - Germany
TA  - Ann Hematol
JT  - Annals of hematology
JID - 9107334
RN  - 0 (E-Selectin)
RN  - 0 (Heparin, Low-Molecular-Weight)
RN  - 0 (Interleukin-1beta)
RN  - 0 (SELE protein, human)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Cell Adhesion/*drug effects/genetics
MH  - Cells, Cultured
MH  - E-Selectin/genetics/metabolism
MH  - Endothelium, Vascular/*drug effects/pathology
MH  - Heparin, Low-Molecular-Weight/*pharmacology
MH  - Human Umbilical Vein Endothelial Cells/drug effects/pathology/physiology
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/genetics/metabolism
MH  - Interleukin-1beta/genetics/metabolism
MH  - Leukemia, Promyelocytic, Acute/complications/genetics/*pathology
MH  - Thrombosis/etiology/*pathology/prevention & control
MH  - Vascular Cell Adhesion Molecule-1/genetics/metabolism
OTO - NOTNLM
OT  - Acute promyelocytic leukemia
OT  - Cancer and thrombosis
OT  - Cell adhesion
OT  - Endothelium
OT  - Low-molecular-weight heparin
EDAT- 2018/05/03 06:00
MHDA- 2018/08/29 06:00
CRDT- 2018/05/03 06:00
PHST- 2017/11/06 00:00 [received]
PHST- 2018/04/21 00:00 [accepted]
PHST- 2018/05/03 06:00 [pubmed]
PHST- 2018/08/29 06:00 [medline]
PHST- 2018/05/03 06:00 [entrez]
AID - 10.1007/s00277-018-3343-4 [pii]
AID - 10.1007/s00277-018-3343-4 [doi]
PST - ppublish
SO  - Ann Hematol. 2018 Sep;97(9):1555-1562. doi: 10.1007/s00277-018-3343-4. Epub 2018 
      May 1.