PMID- 29717637
OWN - NLM
STAT- MEDLINE
DCOM- 20181109
LR  - 20210909
IS  - 1943-4936 (Electronic)
IS  - 1040-6387 (Print)
IS  - 1040-6387 (Linking)
VI  - 30
IP  - 4
DP  - 2018 Jul
TI  - Validation of a radioimmunoassay of serum trypsin-like immunoreactivity in 
      ferrets.
PG  - 517-522
LID - 10.1177/1040638718774387 [doi]
AB  - Measurement of serum trypsin-like immunoreactivity (TLI) is used to assess 
      exocrine pancreatic function in dogs and cats. Ferrets ( Mustela putorius furo) 
      serve as valuable animal models for human diseases such as cystic fibrosis and 
      other pulmonary diseases, and may be a useful model of other diseases including 
      pancreatitis. We developed and analytically validated a competitive 
      radioimmunoassay (RIA) for measurement of TLI in ferret serum by determination of 
      analytical sensitivity, assay linearity, accuracy of spiking recovery, precision, 
      and reproducibility. Analytical sensitivity of the assay was 0.55 mug/L. 
      Observed-to-expected (O/E) ratio for dilutional parallelism was 90.2-127.9% 
      (mean: 108.1 +/- 11.9%). The O/E ratio for spiking recovery was 94.5-113.0% (mean: 
      103.9 +/- 7.2%). The intra- and inter-assay coefficients of variation (CVs) were 
      2.7-5.7% and 3.5-8.2%, respectively. The reference interval (RI) for serum TLI 
      derived from 31 healthy ferrets was 28-115 mug/L; the 90% confidence interval for 
      the lower and upper limits of the RI were 10.0-32.1 mug/L and 103-126 mug/L, 
      respectively. This TLI RIA is analytically sensitive, sufficiently linear, 
      accurate, precise, and reproducible for the measurement of TLI in ferret serum 
      samples.
FAU - Bridges, Cory S
AU  - Bridges CS
AUID- ORCID: 0000-0002-5200-2513
AD  - Gastrointestinal Laboratory, Texas A&M University, College Station, TX (Bridges, 
      Miller, Lidbury, Suchodolski, Steiner).
AD  - Anatomy and Cell Biology, University of Iowa, Carver College of Medicine, Iowa 
      City, IA (Engelhardt, Yi).
FAU - Miller, Pamela S
AU  - Miller PS
AD  - Gastrointestinal Laboratory, Texas A&M University, College Station, TX (Bridges, 
      Miller, Lidbury, Suchodolski, Steiner).
AD  - Anatomy and Cell Biology, University of Iowa, Carver College of Medicine, Iowa 
      City, IA (Engelhardt, Yi).
FAU - Lidbury, Jonathan A
AU  - Lidbury JA
AD  - Gastrointestinal Laboratory, Texas A&M University, College Station, TX (Bridges, 
      Miller, Lidbury, Suchodolski, Steiner).
AD  - Anatomy and Cell Biology, University of Iowa, Carver College of Medicine, Iowa 
      City, IA (Engelhardt, Yi).
FAU - Suchodolski, Jan S
AU  - Suchodolski JS
AD  - Gastrointestinal Laboratory, Texas A&M University, College Station, TX (Bridges, 
      Miller, Lidbury, Suchodolski, Steiner).
AD  - Anatomy and Cell Biology, University of Iowa, Carver College of Medicine, Iowa 
      City, IA (Engelhardt, Yi).
FAU - Yi, Yaling
AU  - Yi Y
AD  - Gastrointestinal Laboratory, Texas A&M University, College Station, TX (Bridges, 
      Miller, Lidbury, Suchodolski, Steiner).
AD  - Anatomy and Cell Biology, University of Iowa, Carver College of Medicine, Iowa 
      City, IA (Engelhardt, Yi).
FAU - Engelhardt, John F
AU  - Engelhardt JF
AD  - Gastrointestinal Laboratory, Texas A&M University, College Station, TX (Bridges, 
      Miller, Lidbury, Suchodolski, Steiner).
AD  - Anatomy and Cell Biology, University of Iowa, Carver College of Medicine, Iowa 
      City, IA (Engelhardt, Yi).
FAU - Steiner, Jorg M
AU  - Steiner JM
AD  - Gastrointestinal Laboratory, Texas A&M University, College Station, TX (Bridges, 
      Miller, Lidbury, Suchodolski, Steiner).
AD  - Anatomy and Cell Biology, University of Iowa, Carver College of Medicine, Iowa 
      City, IA (Engelhardt, Yi).
LA  - eng
GR  - P30 DK054759/DK/NIDDK NIH HHS/United States
GR  - P30 ES005605/ES/NIEHS NIH HHS/United States
GR  - R24 DK096518/DK/NIDDK NIH HHS/United States
GR  - R24 HL123482/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Validation Study
DEP - 20180502
PL  - United States
TA  - J Vet Diagn Invest
JT  - Journal of veterinary diagnostic investigation : official publication of the 
      American Association of Veterinary Laboratory Diagnosticians, Inc
JID - 9011490
RN  - EC 3.4.21.4 (Trypsin)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Exocrine Pancreatic Insufficiency/blood/veterinary
MH  - Ferrets/*blood
MH  - Pancreas/*metabolism
MH  - Rabbits
MH  - Radioimmunoassay/*veterinary
MH  - Reference Values
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Species Specificity
MH  - Trypsin/*blood
PMC - PMC6343499
MID - NIHMS1004736
OTO - NOTNLM
OT  - Ferrets
OT  - radioimmunoassay
OT  - trypsin
OT  - trypsin-like immunoreactivity
OT  - trypsinogen
COIS- Declaration of conflicting interests: The Gastrointestinal Laboratory at Texas 
      A&M University provides serum trypsin-like immunoreactivity (TLI) tests for dogs 
      and cats on a fee-for-service basis.
EDAT- 2018/05/03 06:00
MHDA- 2018/11/10 06:00
PMCR- 2019/05/02
CRDT- 2018/05/03 06:00
PHST- 2018/05/03 06:00 [pubmed]
PHST- 2018/11/10 06:00 [medline]
PHST- 2018/05/03 06:00 [entrez]
PHST- 2019/05/02 00:00 [pmc-release]
AID - 10.1177_1040638718774387 [pii]
AID - 10.1177/1040638718774387 [doi]
PST - ppublish
SO  - J Vet Diagn Invest. 2018 Jul;30(4):517-522. doi: 10.1177/1040638718774387. Epub 
      2018 May 2.