PMID- 29718193
OWN - NLM
STAT- MEDLINE
DCOM- 20191031
LR  - 20200306
IS  - 1758-535X (Electronic)
IS  - 1079-5006 (Print)
IS  - 1079-5006 (Linking)
VI  - 74
IP  - 1
DP  - 2019 Jan 1
TI  - Transcriptional Profiling of Age-Associated Gene Expression Changes in Human 
      Circulatory CD1c+ Myeloid Dendritic Cell Subset.
PG  - 9-15
LID - 10.1093/gerona/gly106 [doi]
AB  - Immune dysfunction is a hallmark of aging and is thought to be responsible for 
      the age-associated diseases. Dendritic cells (DCs) of the immune system function 
      as initiators and regulators of the immune responses. Recent studies have 
      highlighted the division of labor between various DC subsets. CD1c+ DC subset has 
      emerged as a major inducer of CD4 T cell response. There is a scarcity of 
      information regarding the age-associated changes in the functions of DC subsets 
      in the elderly. Here, we investigated the changes in transcriptional profile of 
      CD1c+ DC subset from healthy aged and young individuals using RNA sequencing. Our 
      results suggest that majority of the genes in DCs are upregulated with age. 
      Glucose transport, GPCR, and potassium channel genes are all upregulated in DCs 
      from aged as compared to young indicating an enhanced activation state of DCs 
      from aged individuals. The expression of histones, small nucleolar RNA H/ACA box 
      (SNORA) and small nucleolar RNA C/D/box (SNORD), and long non-coding RNA (lncRNA) 
      is also substantially upregulated in the DCs from aged. In contrast, the 
      antigen-presenting and energy generating pathways are downregulated. In summary, 
      DCs from aged subjects display an activated state coupled with reduced antigen 
      presentation which may be responsible for age-associate immune dysfunction.
FAU - Rahmatpanah, Farah
AU  - Rahmatpanah F
AD  - Department of Pathology, University of California, Irvine.
FAU - Agrawal, Sudhanshu
AU  - Agrawal S
AD  - Division of Basic and Clinical Immunology, Department of Medicine, University of 
      California, Irvine.
FAU - Scarfone, Vanessa M
AU  - Scarfone VM
AD  - Stem Cell Research Center, University of California, Irvine.
FAU - Kapadia, Sameer
AU  - Kapadia S
AD  - Division of Basic and Clinical Immunology, Department of Medicine, University of 
      California, Irvine.
FAU - Mercola, Dan
AU  - Mercola D
AD  - Department of Pathology, University of California, Irvine.
FAU - Agrawal, Anshu
AU  - Agrawal A
AD  - Division of Basic and Clinical Immunology, Department of Medicine, University of 
      California, Irvine.
LA  - eng
GR  - R01 AG045216/AG/NIA NIH HHS/United States
GR  - UL1 TR001414/TR/NCATS NIH HHS/United States
GR  - UL1 TR000153/TR/NCATS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Gerontol A Biol Sci Med Sci
JT  - The journals of gerontology. Series A, Biological sciences and medical sciences
JID - 9502837
RN  - 0 (Antigens, CD1)
RN  - 0 (CD1C protein, human)
RN  - 0 (Glycoproteins)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aging/*genetics
MH  - Antigens, CD1/biosynthesis/*genetics
MH  - Dendritic Cells/cytology/*metabolism
MH  - Female
MH  - Flow Cytometry
MH  - Gene Expression Profiling/*methods
MH  - *Gene Expression Regulation
MH  - Glycoproteins/biosynthesis/*genetics
MH  - Humans
MH  - Immunity, Cellular/*genetics
MH  - Male
MH  - Middle Aged
MH  - RNA/*genetics
MH  - Transcriptome/genetics
PMC - PMC6298184
EDAT- 2018/05/03 06:00
MHDA- 2019/11/02 06:00
PMCR- 2019/04/28
CRDT- 2018/05/03 06:00
PHST- 2017/10/26 00:00 [received]
PHST- 2018/04/26 00:00 [accepted]
PHST- 2018/05/03 06:00 [pubmed]
PHST- 2019/11/02 06:00 [medline]
PHST- 2018/05/03 06:00 [entrez]
PHST- 2019/04/28 00:00 [pmc-release]
AID - 4989886 [pii]
AID - gly106 [pii]
AID - 10.1093/gerona/gly106 [doi]
PST - ppublish
SO  - J Gerontol A Biol Sci Med Sci. 2019 Jan 1;74(1):9-15. doi: 10.1093/gerona/gly106.