PMID- 29718708 OWN - NLM STAT- MEDLINE DCOM- 20190401 LR - 20190401 IS - 1530-6860 (Electronic) IS - 0892-6638 (Linking) VI - 32 IP - 10 DP - 2018 Oct TI - Plasma metabolome analysis identifies distinct human metabotypes in the postprandial state with different susceptibility to weight loss-mediated metabolic improvements. PG - 5447-5458 LID - 10.1096/fj.201800330R [doi] AB - Health has been defined as the capability of the organism to adapt to challenges. In this study, we tested to what extent comprehensively phenotyped individuals reveal differences in metabolic responses to a standardized mixed meal tolerance test (MMTT) and how these responses change when individuals experience moderate weight loss. Metabolome analysis was used in 70 healthy individuals. with profiling of approximately 300 plasma metabolites during an MMTT over 8 h. Multivariate analysis of plasma markers of fatty acid catabolism identified 2 distinct metabotype clusters (A and B). Individuals from metabotype B showed slower glucose clearance, had increased intra-abdominal adipose tissue mass and higher hepatic lipid levels when compared with individuals from metabotype A. An NMR-based urine analysis revealed that these individuals also to have a less healthy dietary pattern. After a weight loss of approximately 5.6 kg over 12 wk, only the subjects from metabotype B showed positive changes in the glycemic response during the MMTT and in markers of metabolic diseases. Our study in healthy individuals demonstrates that more comprehensive phenotyping can reveal discrete metabotypes with different outcomes in a dietary intervention and that markers of lipid catabolism in plasma could allow early detection of the metabolic syndrome.-Fiamoncini, J., Rundle, M., Gibbons, H., Thomas, E. L., Geillinger-Kastle, K., Bunzel, D., Trezzi, J.-P., Kiselova-Kaneva, Y., Wopereis, S., Wahrheit, J., Kulling, S. E., Hiller, K., Sonntag, D., Ivanova, D., van Ommen, B., Frost, G., Brennan, L., Bell, J. Daniel, H. Plasma metabolome analysis identifies distinct human metabotypes in the postprandial state with different susceptibility to weight loss-mediated metabolic improvements. FAU - Fiamoncini, Jarlei AU - Fiamoncini J AD - Department of Food and Nutrition, Technische Universitat Munchen, Freising-Weihenstephan, Germany. FAU - Rundle, Milena AU - Rundle M AD - Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Imperial College London, London, United Kingdom. FAU - Gibbons, Helena AU - Gibbons H AD - University College Dublin (UCD) School of Agriculture and Food Science, Institute of Food and Health, Dublin, Ireland. FAU - Thomas, E Louise AU - Thomas EL AD - Research Centre for Optimal Health, Department of Life Sciences, University of Westminster, London, United Kingdom. FAU - Geillinger-Kastle, Kerstin AU - Geillinger-Kastle K AD - Department of Food and Nutrition, Technische Universitat Munchen, Freising-Weihenstephan, Germany. FAU - Bunzel, Diana AU - Bunzel D AD - Department of Safety and Quality of Fruit and Vegetables, Max Rubner Institut, Karlsruhe, Germany. FAU - Trezzi, Jean-Pierre AU - Trezzi JP AD - Integrated Biobank of Luxembourg, Dudelange, Luxembourg. AD - Centre for Systems Biomedicine, Esch-sur-Alzette, Luxembourg. FAU - Kiselova-Kaneva, Yoana AU - Kiselova-Kaneva Y AD - Department of Biochemistry, Molecular Medicine, and Nutrigenomics, Medical University-Varna, Varna, Bulgaria. FAU - Wopereis, Suzan AU - Wopereis S AD - Department of Microbiology and Systems Biology, Netherlands Organization for Applied Scientific Research (TNO), Zeist, The Netherlands. FAU - Wahrheit, Judith AU - Wahrheit J AD - Biocrates Life Sciences, Innsbruck, Austria. FAU - Kulling, Sabine E AU - Kulling SE AD - Department of Safety and Quality of Fruit and Vegetables, Max Rubner Institut, Karlsruhe, Germany. FAU - Hiller, Karsten AU - Hiller K AD - Braunschweig Integrated Centre of Systems Biology, University of Braunschweig, Braunschweig, Germany. AD - Department of Computational Biology of Infection Research, Helmholtz Centre for Infection Research, Braunschweig, Germany. FAU - Sonntag, Denise AU - Sonntag D AD - Department of Microbiology and Systems Biology, Netherlands Organization for Applied Scientific Research (TNO), Zeist, The Netherlands. FAU - Ivanova, Diana AU - Ivanova D AD - Department of Biochemistry, Molecular Medicine, and Nutrigenomics, Medical University-Varna, Varna, Bulgaria. FAU - van Ommen, Ben AU - van Ommen B AD - Department of Microbiology and Systems Biology, Netherlands Organization for Applied Scientific Research (TNO), Zeist, The Netherlands. FAU - Frost, Gary AU - Frost G AD - Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Imperial College London, London, United Kingdom. FAU - Brennan, Lorraine AU - Brennan L AD - University College Dublin (UCD) School of Agriculture and Food Science, Institute of Food and Health, Dublin, Ireland. FAU - Bell, Jimmy AU - Bell J AD - Research Centre for Optimal Health, Department of Life Sciences, University of Westminster, London, United Kingdom. FAU - Daniel, Hannelore AU - Daniel H AD - Department of Food and Nutrition, Technische Universitat Munchen, Freising-Weihenstephan, Germany. LA - eng PT - Clinical Trial PT - Journal Article PT - Multicenter Study PT - Research Support, Non-U.S. Gov't DEP - 20180502 PL - United States TA - FASEB J JT - FASEB journal : official publication of the Federation of American Societies for Experimental Biology JID - 8804484 SB - IM MH - Female MH - Humans MH - Male MH - Metabolic Syndrome/blood/diagnosis MH - *Metabolome MH - Middle Aged MH - *Postprandial Period MH - *Weight Loss OTO - NOTNLM OT - acylcarnitines OT - dietary challenges OT - metabolomics OT - phenotypic flexibility EDAT- 2018/05/03 06:00 MHDA- 2019/04/02 06:00 CRDT- 2018/05/03 06:00 PHST- 2018/05/03 06:00 [pubmed] PHST- 2019/04/02 06:00 [medline] PHST- 2018/05/03 06:00 [entrez] AID - 10.1096/fj.201800330R [doi] PST - ppublish SO - FASEB J. 2018 Oct;32(10):5447-5458. doi: 10.1096/fj.201800330R. Epub 2018 May 2.