PMID- 2971874
OWN - NLM
STAT- MEDLINE
DCOM- 19881121
LR  - 20190818
IS  - 0300-8177 (Print)
IS  - 0300-8177 (Linking)
VI  - 81
IP  - 2
DP  - 1988 Jun
TI  - Effect of exercise on glycolytic enzymes of Zucker fatty rats.
PG  - 177-86
AB  - The effect of fatigue (running to exhaustion) on the Vmax activity of the key 
      glycolytic enzymes measured at saturating substrate concentrations in muscles, 
      liver and brain of sedentary and trained (running on a treadmill one h/day at 20 
      m/min, five days/week for six months) female Zucker fatty rats and their lean 
      littermates was investigated. In the sedentary rats, fatigue increased the 
      activity of phosphofructokinase (PFK) in the red vastus muscle by 82% in lean, 
      and 120% in obese rats. In the trained rats, fatigue increased PFK activity by 
      28% in the white vastus muscle of lean rats. In the lean animals, hexokinase (HK) 
      activity was decreased by 26% in the red vastus of sedentary rats, and by 29% in 
      the white vastus of trained rats upon fatiguing. Pyruvate kinase (PK) activity 
      was also decreased by 29% in the white vastus of fatigued lean animals. Training 
      by itself had no effect on the activity of glycolytic enzymes, except PK activity 
      which was increased by 27% in the cortex of the lean animals. It is concluded 
      that in the Zucker rat, these glycolytic enzymes may play a differential role in 
      regulating glycolysis during exercise and fatigue; the extent of their 
      involvement differs depending upon the type of tissue studied and exercise. In 
      view of the reported short half-life (7-17 h) of PFK and its covalent 
      modification, it is suggested that the total content and/or phosphorylation 
      status of the enzyme may be affected in animals subjected to long-term fatigue.
FAU - Hanissian, S H
AU  - Hanissian SH
AD  - Department of Pharmacology, Ohio State University, Coll. of Med, Columbus 43210.
FAU - Tejwani, G A
AU  - Tejwani GA
FAU - Mahle, C D
AU  - Mahle CD
FAU - Merola, J A
AU  - Merola JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.11 (Phosphofructokinase-1)
RN  - EC 2.7.1.40 (Pyruvate Kinase)
SB  - IM
MH  - Animals
MH  - Fatigue/enzymology
MH  - Female
MH  - Hexokinase/*metabolism
MH  - Obesity/enzymology
MH  - Phosphofructokinase-1/*metabolism
MH  - *Physical Exertion
MH  - Pyruvate Kinase/*metabolism
MH  - Rats
MH  - Rats, Zucker
EDAT- 1988/06/01 00:00
MHDA- 1988/06/01 00:01
CRDT- 1988/06/01 00:00
PHST- 1988/06/01 00:00 [pubmed]
PHST- 1988/06/01 00:01 [medline]
PHST- 1988/06/01 00:00 [entrez]
AID - 10.1007/BF00219320 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 1988 Jun;81(2):177-86. doi: 10.1007/BF00219320.