PMID- 29719011
OWN - NLM
STAT- MEDLINE
DCOM- 20180926
LR  - 20220331
IS  - 1934-3418 (Electronic)
IS  - 1078-4519 (Linking)
VI  - 47
IP  - 4
DP  - 2018 Apr
TI  - Short-Term Storage of Platelet-Rich Plasma at Room Temperature Does Not Affect 
      Growth Factor or Catabolic Cytokine Concentration.
LID - 10.12788/ajo.2018.0022 [doi]
AB  - The aim of this study was to provide clinical recommendations about the use of 
      platelet-rich plasma (PRP) that was subjected to short-term storage at room 
      temperature. We determined bioactive growth factor and cytokine concentrations as 
      indicators of platelet and white blood cell degranulation in blood and PRP. 
      Additionally, this study sought to validate the use of manual, direct smear 
      analysis as an alternative to automated methods for platelet quantification in 
      PRP. Blood was used to generate low-leukocyte PRP (Llo PRP) or high-leukocyte PRP 
      (Lhi PRP). Blood was either processed immediately or kept at room temperature for 
      2 or 4 hours prior to generation of PRP, which was then held at room temperature 
      for 0, 1, 2, or 4 hours. Subsequently, bioactive transforming growth factor 
      beta-1 and matrix metalloproteinase-9 were measured by ELISA (enzyme-linked 
      immunosorbent assay). Manual and automated platelet counts were performed on all 
      blood and PRP samples. There were no differences in growth factor or cytokine 
      concentration when blood or Llo PRP or Lhi PRP was retained at room temperature 
      for up to 4 hours. Manual, direct smear analysis for platelet quantification was 
      not different from the use of automated machine counting for PRP samples, but in 
      the starting blood samples, manual platelet counts were significantly higher than 
      those generated using automated technology. When there is a delay of up to 4 
      hours in the generation of PRP from blood or in the application of PRP to the 
      patient, bioactive growth factor and cytokine concentrations remain stable in 
      both blood and PRP. A manual direct counting method is a simple, cost-effective, 
      and valid method to measure the contents of the PRP product being delivered to 
      the patient.
FAU - Wilson, Brooke H
AU  - Wilson BH
FAU - Cole, Brian J
AU  - Cole BJ
FAU - Goodale, Margaret B
AU  - Goodale MB
FAU - Fortier, Lisa A
AU  - Fortier LA
AD  - Department of Clinical Sciences, Cornell University, Ithaca, NY. 
      laf4@cornell.edu.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Orthop (Belle Mead NJ)
JT  - American journal of orthopedics (Belle Mead, N.J.)
JID - 9502918
RN  - 0 (Cytokines)
SB  - IM
MH  - Blood Specimen Collection/*methods
MH  - Cytokines/blood
MH  - Humans
MH  - *Platelet-Rich Plasma
MH  - *Temperature
COIS- Authors' Disclosure Statement: The authors report that Arthrex donated syringes 
      for generating platelet-rich plasma. Dr. Fortier reports that she is a paid 
      consultant for Arthrex. Dr. Cole reports that he receives intellectual property 
      royalties from, is a paid consultant, and provides research support to Arthrex. 
      This article was supported by the National Institute of Health and the Harry M. 
      Zweig Memorial Fund for Equine Research. The content is solely the responsibility 
      of the authors and does not necessarily represent the official views of the 
      National Institutes of Health.
EDAT- 2018/05/03 06:00
MHDA- 2018/09/27 06:00
CRDT- 2018/05/03 06:00
PHST- 2018/05/03 06:00 [entrez]
PHST- 2018/05/03 06:00 [pubmed]
PHST- 2018/09/27 06:00 [medline]
AID - 10.12788/ajo.2018.0022 [doi]
PST - ppublish
SO  - Am J Orthop (Belle Mead NJ). 2018 Apr;47(4). doi: 10.12788/ajo.2018.0022.