PMID- 29719531
OWN - NLM
STAT- MEDLINE
DCOM- 20190625
LR  - 20240314
IS  - 1664-3224 (Print)
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 9
DP  - 2018
TI  - Characterization of Chicken Tumor Necrosis Factor-alpha, a Long Missed Cytokine in 
      Birds.
PG  - 605
LID - 10.3389/fimmu.2018.00605 [doi]
LID - 605
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a pleiotropic cytokine playing critical roles 
      in host defense and acute and chronic inflammation. It has been described in 
      fish, amphibians, and mammals but was considered to be absent in the avian 
      genomes. Here, we report on the identification and functional characterization of 
      the avian ortholog. The chicken TNF-alpha (chTNF-alpha) is encoded by a highly GC-rich 
      gene, whose product shares with its mammalian counterpart 45% homology in the 
      extracellular part displaying the characteristic TNF homology domain. Orthologs 
      of chTNF-alpha were identified in the genomes of 12 additional avian species 
      including Palaeognathae and Neognathae, and the synteny of the closely adjacent 
      loci with mammalian TNF-alpha orthologs was demonstrated in the crow (Corvus cornix) 
      genome. In addition to chTNF-alpha, we obtained full sequences for homologs of TNF-alpha 
      receptors 1 and 2 (TNFR1, TNFR2). chTNF-alpha mRNA is strongly induced by 
      lipopolysaccharide (LPS) stimulation of monocyte derived, splenic and bone marrow 
      macrophages, and significantly upregulated in splenic tissue in response to i.v. 
      LPS treatment. Activation of T-lymphocytes by TCR crosslinking induces chTNF-alpha 
      expression in CD4(+) but not in CD8(+) cells. To gain insights into its 
      biological activity, we generated recombinant chTNF-alpha in eukaryotic and 
      prokaryotic expression systems. Both, the full-length cytokine and the 
      extracellular domain rapidly induced an NFkappaB-luciferase reporter in stably 
      transfected CEC-32 reporter cells. Collectively, these data provide strong 
      evidence for the existence of a fully functional TNF-alpha/TNF-alpha receptor system in 
      birds thus filling a gap in our understanding of the evolution of cytokine 
      systems.
FAU - Rohde, Franziska
AU  - Rohde F
AD  - Department of Veterinary Science, Ludwig-Maximilians-Universitat, Munich, 
      Germany.
FAU - Schusser, Benjamin
AU  - Schusser B
AD  - Reproductive Biotechnology, Department of Animal Sciences, Technical University 
      Munich, Munich, Germany.
FAU - Hron, Tomas
AU  - Hron T
AD  - Laboratory of Viral and Cellular Genetics, Institute of Molecular Genetics of the 
      Czech Academy of Sciences, Prague, Czechia.
FAU - Farkasova, Helena
AU  - Farkasova H
AD  - Laboratory of Viral and Cellular Genetics, Institute of Molecular Genetics of the 
      Czech Academy of Sciences, Prague, Czechia.
FAU - Plachy, Jiri
AU  - Plachy J
AD  - Laboratory of Viral and Cellular Genetics, Institute of Molecular Genetics of the 
      Czech Academy of Sciences, Prague, Czechia.
FAU - Hartle, Sonja
AU  - Hartle S
AD  - Department of Veterinary Science, Ludwig-Maximilians-Universitat, Munich, 
      Germany.
FAU - Hejnar, Jiri
AU  - Hejnar J
AD  - Laboratory of Viral and Cellular Genetics, Institute of Molecular Genetics of the 
      Czech Academy of Sciences, Prague, Czechia.
FAU - Elleder, Daniel
AU  - Elleder D
AD  - Laboratory of Viral and Cellular Genetics, Institute of Molecular Genetics of the 
      Czech Academy of Sciences, Prague, Czechia.
FAU - Kaspers, Bernd
AU  - Kaspers B
AD  - Department of Veterinary Science, Ludwig-Maximilians-Universitat, Munich, 
      Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180417
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Avian Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Avian Proteins/*genetics/metabolism
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - Cells, Cultured
MH  - Chickens/*immunology
MH  - Cloning, Molecular
MH  - Crows/immunology
MH  - GC Rich Sequence/genetics
MH  - Humans
MH  - Macrophages/*immunology
MH  - Mammals/immunology
MH  - NF-kappa B/metabolism
MH  - Palaeognathae/immunology
MH  - Receptors, Tumor Necrosis Factor/*genetics/metabolism
MH  - Sequence Alignment
MH  - Tumor Necrosis Factor-alpha/*genetics
PMC - PMC5913325
OTO - NOTNLM
OT  - avian
OT  - biological activity
OT  - chicken
OT  - missing gene
OT  - tumor necrosis factor-alpha
OT  - tumor necrosis factor-alpha receptors
EDAT- 2018/05/03 06:00
MHDA- 2018/05/03 06:01
PMCR- 2018/01/01
CRDT- 2018/05/03 06:00
PHST- 2017/12/05 00:00 [received]
PHST- 2018/03/09 00:00 [accepted]
PHST- 2018/05/03 06:00 [entrez]
PHST- 2018/05/03 06:00 [pubmed]
PHST- 2018/05/03 06:01 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2018.00605 [doi]
PST - epublish
SO  - Front Immunol. 2018 Apr 17;9:605. doi: 10.3389/fimmu.2018.00605. eCollection 
      2018.