PMID- 29720871
OWN - NLM
STAT- MEDLINE
DCOM- 20181011
LR  - 20220317
IS  - 1177-8881 (Electronic)
IS  - 1177-8881 (Linking)
VI  - 12
DP  - 2018
TI  - Islet protection and amelioration of type 2 diabetes mellitus by treatment with 
      quercetin from the flowers of Edgeworthia gardneri.
PG  - 955-966
LID - 10.2147/DDDT.S153898 [doi]
AB  - BACKGROUND AND PURPOSE: The traditional Chinese medicine - the flower of 
      Edgeworthia gardneri - is reported as an effective therapeutic for type 2 
      diabetes mellitus (T2DM). Nevertheless, most constituents of the flowers of E. 
      gardneri have not yet been studied. This study was conducted to investigate the 
      effect of quercetin extracted from the flowers of E. gardneri on islet protection 
      and amelioration in T2DM and explore its mechanism. METHOD: Quercetin was 
      extracted from the flowers of E. gardneri and verified by high-performance liquid 
      chromatography. Quercetin or crude extract's effect on insulin secretion was 
      investigated. ERK1/2 and phospho-ERK1/2 were detected by Western blot analysis, 
      and fluo-3 AM was used to detect intracellular Ca(2+). The anti-apoptosis effect 
      of quercetin or crude extract on MIN-6 cells was investigated by thiazolyl blue 
      tetrazolium bromide (MTT) assay and flow cytometry analysis. Activation of 
      caspases and expression of Bcl-2 and BAX were tested by Western blot analysis. In 
      addition, the mitochondrial membrane potential was determined by JC-1 probe. 
      Moreover, in vivo activity was also tested in db/db mice. RESULTS: A quercetin 
      level of >10 mumol/L could induce insulin secretion. Intracellular Ca(2+) and 
      ERK1/2 were involved in the signaling pathway of quercetin-induced insulin 
      secretion. We also observed that quercetin could inhibit palmitic acid-induced 
      cell apoptosis via suppressing the activation of caspase-3, -9, -12; increasing 
      the ratio of Bcl-2/BAX and reversing the impaired mitochondrial membrane 
      potential. Crude extract's effect on insulin secretion was similar to that of 
      pure extracted quercetin, while it possessed higher anti-apoptosis activity. 
      Additionally, intraperitoneal glucose tolerance, plasma insulin level, hepatic 
      triglyceride, hepatic glycogen and the pathological histology of both pancreatic 
      islet and liver in db/db mice were significantly improved by the administration 
      of the extracted quercetin. CONCLUSION: Our study indicated that quercetin 
      extracted from the flowers of E. gardneri exerted excellent properties in islet 
      protection and amelioration.
FAU - Zhuang, Manjiao
AU  - Zhuang M
AD  - Department of Pharmacy, Sichuan University, Chengdu, People's Republic of China.
FAU - Qiu, Honghong
AU  - Qiu H
AD  - Collaborative Innovation Center of Sichuan for Elderly Care and Health, Chengdu 
      Medical College, Chengdu, China.
FAU - Li, Ping
AU  - Li P
AD  - Collaborative Innovation Center of Sichuan for Elderly Care and Health, Chengdu 
      Medical College, Chengdu, China.
FAU - Hu, Lihua
AU  - Hu L
AD  - Collaborative Innovation Center of Sichuan for Elderly Care and Health, Chengdu 
      Medical College, Chengdu, China.
FAU - Wang, Yayu
AU  - Wang Y
AD  - Department of Cell Biology, Institute of Biological Medicine, Jinan University, 
      Guangzhou, People's Republic of China.
FAU - Rao, Lei
AU  - Rao L
AD  - Collaborative Innovation Center of Sichuan for Elderly Care and Health, Chengdu 
      Medical College, Chengdu, China.
LA  - eng
PT  - Journal Article
DEP - 20180423
PL  - New Zealand
TA  - Drug Des Devel Ther
JT  - Drug design, development and therapy
JID - 101475745
RN  - 0 (Antioxidants)
RN  - 0 (Insulin)
RN  - 2V16EO95H1 (Palmitic Acid)
RN  - 9IKM0I5T1E (Quercetin)
SB  - IM
MH  - Animals
MH  - Antioxidants/administration & dosage/isolation & purification/*pharmacology
MH  - Apoptosis/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Diabetes Mellitus, Experimental/*drug therapy/metabolism
MH  - Diabetes Mellitus, Type 2/*drug therapy/metabolism
MH  - Flowers/*chemistry
MH  - Humans
MH  - Insulin/blood/*metabolism
MH  - Medicine, Chinese Traditional
MH  - Membrane Potential, Mitochondrial/drug effects
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Obese
MH  - Palmitic Acid/antagonists & inhibitors/pharmacology
MH  - Quercetin/administration & dosage/isolation & purification/*pharmacology
MH  - Thymelaeaceae/*chemistry
PMC - PMC5918626
OTO - NOTNLM
OT  - Edgeworthia gardneri
OT  - anti-apoptosis
OT  - insulin secretion
OT  - quercetin
OT  - type 2 diabetes mellitus
COIS- Disclosure The authors report no conflicts of interest in this work.
EDAT- 2018/05/04 06:00
MHDA- 2018/10/12 06:00
PMCR- 2018/04/23
CRDT- 2018/05/04 06:00
PHST- 2018/05/04 06:00 [entrez]
PHST- 2018/05/04 06:00 [pubmed]
PHST- 2018/10/12 06:00 [medline]
PHST- 2018/04/23 00:00 [pmc-release]
AID - dddt-12-955 [pii]
AID - 10.2147/DDDT.S153898 [doi]
PST - epublish
SO  - Drug Des Devel Ther. 2018 Apr 23;12:955-966. doi: 10.2147/DDDT.S153898. 
      eCollection 2018.