PMID- 29722258
OWN - NLM
STAT- MEDLINE
DCOM- 20190610
LR  - 20190613
IS  - 1535-3907 (Electronic)
IS  - 1535-3893 (Linking)
VI  - 17
IP  - 6
DP  - 2018 Jun 1
TI  - Diet Impacts Pre-implantation Histotroph Proteomes in Beef Cattle.
PG  - 2144-2155
LID - 10.1021/acs.jproteome.8b00077 [doi]
AB  - In ruminants, the period from fertilization to implantation is relatively 
      prolonged, and the survival of embryos depends on uterine secretions known as 
      histotroph. Our objective was to determine if the pre-breeding diet affected 
      histotroph proteomes in beef cattle. Cows were assigned to one of four diets: a 
      control diet (CON), a high-protein diet (PROT), a high-fat diet (OIL), or a 
      high-protein and high-fat diet (PROT + OIL). After 185 days on these diets, an 
      intravaginal progesterone implant (CIDR) was inserted for 7 days. At 9 days after 
      CIDR removal, animals with a corpus luteum were selected ( n = 16; 4 per 
      treatment). Proteins were isolated from the histotroph collected by uterine 
      lavage and analyzed with liquid chromatography-tandem mass spectrometry. Over 
      2000 proteins were expressed ( n >/= 3 cows per treatment), with 1239 proteins 
      being common among all of the groups. There were 20, 37, 85, and 123 proteins 
      unique to CON, PROT + OIL, PROT, and OIL, respectively. Relative to CON, 23, 14, 
      and 51 proteins were differentially expressed in PROT + OIL, PROT, and OIL, 
      respectively. Functional analysis found that 53% of histotroph proteins were 
      categorized as extracellular exosome, 3.28% as cell-cell adhesion, and 17.4% in 
      KEGG metabolic pathways. Differences in proteomes among treatments support the 
      idea that pre-breeding diet affects histotroph. Understanding the impact of diet 
      on histotroph proteins may help improve conception rates.
FAU - Harlow, KaLynn
AU  - Harlow K
FAU - Taylor, Emily
AU  - Taylor E
FAU - Casey, Theresa
AU  - Casey T
AUID- ORCID: 0000-0002-8835-3550
FAU - Hedrick, Victoria
AU  - Hedrick V
FAU - Sobreira, Tiago
AU  - Sobreira T
FAU - Aryal, Uma K
AU  - Aryal UK
FAU - Lemenager, Ronald P
AU  - Lemenager RP
FAU - Funnell, Bethany
AU  - Funnell B
FAU - Stewart, Kara
AU  - Stewart K
AUID- ORCID: 0000-0002-6223-8159
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180518
PL  - United States
TA  - J Proteome Res
JT  - Journal of proteome research
JID - 101128775
RN  - 0 (Proteome)
SB  - IM
MH  - Animals
MH  - *Breeding
MH  - Cattle
MH  - Chromatography, Liquid
MH  - *Diet
MH  - Embryo Implantation
MH  - Female
MH  - *Proteome
MH  - Red Meat
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - LC-MS/MS
OT  - cow
OT  - histotroph
OT  - liquid chromatography-tandem mass spectrometry
OT  - nutrition
OT  - proteome
EDAT- 2018/05/04 06:00
MHDA- 2019/06/14 06:00
CRDT- 2018/05/04 06:00
PHST- 2018/05/04 06:00 [pubmed]
PHST- 2019/06/14 06:00 [medline]
PHST- 2018/05/04 06:00 [entrez]
AID - 10.1021/acs.jproteome.8b00077 [doi]
PST - ppublish
SO  - J Proteome Res. 2018 Jun 1;17(6):2144-2155. doi: 10.1021/acs.jproteome.8b00077. 
      Epub 2018 May 18.