PMID- 29723214
OWN - NLM
STAT- MEDLINE
DCOM- 20180806
LR  - 20240314
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 5
DP  - 2018
TI  - Micro/nano hierarchical structured titanium treated by NH4OH/H2O2 for enhancing 
      cell response.
PG  - e0196366
LID - 10.1371/journal.pone.0196366 [doi]
LID - e0196366
AB  - In this paper, two kinds of titanium surfaces with novel micro/nano hierarchical 
      structures, namely Etched (E) surface and Sandblast and etched (SE) surface, were 
      successfully fabricated by NH4OH and H2O2 mixture. And their cellular responses 
      of MG63 were investigated compared with Sandblast and acid-etching (SLA) surface. 
      Scanning electron microscope (SEM), Surface profiler, X-ray photoelectron 
      spectroscopy (XPS), and Contact angle instrument were employed to assess the 
      surface morphologies, roughness, chemistry and wettability respectively. 
      Hierarchical structures with micro holes of 10-30 mum in diameter and nano pits of 
      tens of nanometers in diameter formed on both E and SE surfaces. The size of 
      micro holes is very close to osteoblast cell, which makes them wonderful beds for 
      osteoblast. Moreover, these two kinds of surfaces possess similar roughness and 
      superior hydrophilicity to SLA. Reactive oxygen species were detected on E and SE 
      surface, and thus considerable antimicrobial performance and well fixation can be 
      speculated on them. The cell experiments also demonstrated a boost in cell 
      attachment, and that proliferation and osteogenic differentiation were achieved 
      on them, especially on SE surface. The results indicate that the treatment of 
      pure titanium with H2O2/NH4OH is an effective technique to improve the initial 
      stability of implants and enhance the osseointegration, which may be a promising 
      surface treatment to titanium implant.
FAU - Yuan, Xin
AU  - Yuan X
AD  - State Key Laboratory of Powder Metallurgy, School of Materials Science and 
      Engineering, Central South University, Changsha, PR China.
FAU - Kang, Yi
AU  - Kang Y
AD  - The Third Xiangya Hospital, Central South University, Changsha, PR China.
FAU - Zuo, Jun
AU  - Zuo J
AD  - Xiangya Stomatological Hospital, Central South University, Changsha, PR China.
FAU - Xie, Youneng
AU  - Xie Y
AD  - State Key Laboratory of Powder Metallurgy, School of Materials Science and 
      Engineering, Central South University, Changsha, PR China.
FAU - Ma, Li
AU  - Ma L
AD  - State Key Laboratory of Powder Metallurgy, School of Materials Science and 
      Engineering, Central South University, Changsha, PR China.
FAU - Ren, Xuelei
AU  - Ren X
AD  - State Key Laboratory of Powder Metallurgy, School of Materials Science and 
      Engineering, Central South University, Changsha, PR China.
FAU - Bian, Zeyu
AU  - Bian Z
AD  - State Key Laboratory of Powder Metallurgy, School of Materials Science and 
      Engineering, Central South University, Changsha, PR China.
FAU - Wei, Qiuping
AU  - Wei Q
AUID- ORCID: 0000-0002-8109-5982
AD  - State Key Laboratory of Powder Metallurgy, School of Materials Science and 
      Engineering, Central South University, Changsha, PR China.
FAU - Zhou, Kechao
AU  - Zhou K
AD  - State Key Laboratory of Powder Metallurgy, School of Materials Science and 
      Engineering, Central South University, Changsha, PR China.
FAU - Wang, Xiyang
AU  - Wang X
AD  - Hunan Provincial Engineering Laboratory for High-performance Bio-engineered 
      Biomimetic Bone Materials, Xiangya Hospital, Central South University, Changsha, 
      PR China.
FAU - Yu, Zhiming
AU  - Yu Z
AD  - State Key Laboratory of Powder Metallurgy, School of Materials Science and 
      Engineering, Central South University, Changsha, PR China.
AD  - Hunan Provincial Engineering Laboratory for High-performance Bio-engineered 
      Biomimetic Bone Materials, Xiangya Hospital, Central South University, Changsha, 
      PR China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180503
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 5138Q19F1X (Ammonium Hydroxide)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - D1JT611TNE (Titanium)
SB  - IM
MH  - Ammonium Hydroxide
MH  - Cell Adhesion
MH  - Cell Differentiation
MH  - Cell Line
MH  - Cell Proliferation
MH  - Humans
MH  - Hydrogen Peroxide
MH  - Metal Nanoparticles/*chemistry
MH  - Microscopy, Electron, Scanning
MH  - Nanotechnology
MH  - Osseointegration
MH  - Osteoblasts/cytology
MH  - *Prostheses and Implants
MH  - Surface Properties
MH  - Titanium/*chemistry
PMC - PMC5933712
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2018/05/04 06:00
MHDA- 2018/08/07 06:00
PMCR- 2018/05/03
CRDT- 2018/05/04 06:00
PHST- 2018/02/10 00:00 [received]
PHST- 2018/04/11 00:00 [accepted]
PHST- 2018/05/04 06:00 [entrez]
PHST- 2018/05/04 06:00 [pubmed]
PHST- 2018/08/07 06:00 [medline]
PHST- 2018/05/03 00:00 [pmc-release]
AID - PONE-D-18-04538 [pii]
AID - 10.1371/journal.pone.0196366 [doi]
PST - epublish
SO  - PLoS One. 2018 May 3;13(5):e0196366. doi: 10.1371/journal.pone.0196366. 
      eCollection 2018.