PMID- 29723817 OWN - NLM STAT- MEDLINE DCOM- 20181001 LR - 20181001 IS - 1873-4367 (Electronic) IS - 0927-7765 (Linking) VI - 167 DP - 2018 Jul 1 TI - Extracellular matrix-coated polyethersulfone-TPGS hollow fiber membranes showing improved biocompatibility and uremic toxins removal for bioartificial kidney application. PG - 457-467 LID - S0927-7765(18)30253-4 [pii] LID - 10.1016/j.colsurfb.2018.04.043 [doi] AB - In this study, L-3, 4-dihydroxyphenylalanine and human collagen type IV were coated over the outer surface of the custom-made hollow fiber membranes (HFMs) with the objective of simultaneously improving biocompatibility leading to proliferation of human embryonic kidney cells-293 (HEK-293) and improving separation of uremic toxins, thereby making them suitable for bioartificial kidney application. Physicochemical characterization showed the development of coated HFMs, resulting in low hemolysis (0.25 +/- 0.10%), low SC5b-9 marker level (7.95 +/- 1.50 ng/mL), prolonged blood coagulation time, and minimal platelet adhesion, which indicated their improved human blood compatibility. Scanning electron microscopy and confocal laser scanning microscopy showed significantly improved attachment and proliferation of HEK-293 cells on the outer surface of the coated HFMs, which was supported by the results of glucose consumption and MTT cell proliferation assay. The solute rejection profile of these coated HFMs was compared favorably with that of the commercial dialyzer membranes. These coated HFMs showed a remarkable 1.6-3.2 fold improvement in reduction ratio of uremic toxins as compared to standard dialyzer membranes. These results clearly demonstrated that these extracellular matrix-coated HFMs can be a potential biocompatible substrate for the attachment and proliferation of HEK-293 cells and removal of uremic toxins from the simulated blood, which may find future application for bioartificial renal assist device. CI - Copyright (c) 2018 Elsevier B.V. All rights reserved. FAU - Modi, Akshay AU - Modi A AD - Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai 400076, India. FAU - Verma, Surendra Kumar AU - Verma SK AD - Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai 400076, India. FAU - Bellare, Jayesh AU - Bellare J AD - Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai 400076, India; Wadhwani Research Centre for Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, India; Centre for Research in Nanotechnology & Science, Indian Institute of Technology Bombay, Mumbai 400076, India. Electronic address: jb@iitb.ac.in. LA - eng PT - Journal Article DEP - 20180424 PL - Netherlands TA - Colloids Surf B Biointerfaces JT - Colloids and surfaces. B, Biointerfaces JID - 9315133 RN - 0 (Coated Materials, Biocompatible) RN - 0 (Membranes, Artificial) RN - 0 (Polymers) RN - 0 (Sulfones) RN - 1406-18-4 (Vitamin E) RN - 25667-42-9 (polyether sulfone) RN - O03S90U1F2 (tocophersolan) SB - IM MH - Cell Proliferation/drug effects MH - Coated Materials, Biocompatible/*chemistry/pharmacology MH - Extracellular Matrix/*chemistry MH - HEK293 Cells MH - Hemolysis/drug effects MH - Humans MH - Kidney/drug effects/metabolism MH - *Membranes, Artificial MH - Microscopy, Confocal MH - Microscopy, Electron, Scanning MH - Platelet Adhesiveness/drug effects MH - Polymers/*chemistry MH - Renal Dialysis/methods MH - Sulfones/*chemistry MH - Uremia/prevention & control MH - Vitamin E/*chemistry OTO - NOTNLM OT - Biocompatibility OT - Extracellular matrix coating OT - Hollow fiber membranes OT - Human embryonic kidney-293 cells OT - Polyethersulfone OT - TPGS OT - Uremic toxins separation EDAT- 2018/05/04 06:00 MHDA- 2018/10/03 06:00 CRDT- 2018/05/04 06:00 PHST- 2018/02/21 00:00 [received] PHST- 2018/04/15 00:00 [revised] PHST- 2018/04/23 00:00 [accepted] PHST- 2018/05/04 06:00 [pubmed] PHST- 2018/10/03 06:00 [medline] PHST- 2018/05/04 06:00 [entrez] AID - S0927-7765(18)30253-4 [pii] AID - 10.1016/j.colsurfb.2018.04.043 [doi] PST - ppublish SO - Colloids Surf B Biointerfaces. 2018 Jul 1;167:457-467. doi: 10.1016/j.colsurfb.2018.04.043. Epub 2018 Apr 24.