PMID- 29725470
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 15
IP  - 5
DP  - 2018 May
TI  - Mutant monocyte chemoattractant protein-1 protein (7ND) inhibits osteoclast 
      differentiation and reduces oral squamous carcinoma cell bone invasion.
PG  - 7760-7768
LID - 10.3892/ol.2018.8308 [doi]
AB  - The seven-amino acid truncated (7ND) protein is an N-terminal deletion mutant of 
      monocyte chemoattractant protein-1 (MCP-1) and it functions as a 
      dominant-negative inhibitor. 7ND and wild-type MCP-1 form a heterodimer, which 
      binds to MCP-1 receptors and inhibits monocyte chemotaxis. In the present study, 
      the 7ND protein was cloned, expressed and purified. An MTT assay revealed that 
      the proliferation of oral squamous cell carcinoma (OSCC) SCC25 cells was not 
      affected following 3 days of treatment with synthetic 7ND protein. Serial 
      dilutions of the 7ND protein were tested for monocyte migration and osteoclast 
      differentiation, and tartrate-resistant acid phosphatase staining demonstrated 
      that significantly fewer osteoclasts were differentiated from cluster of 
      differentiation 14(+) (CD14(+)) monocytes using magnetic activated cell sorting. 
      Immunofluorescence confirmed these results and significantly less F-actin 
      staining was observed in 7ND-treated osteoclasts. Furthermore, bone invasion was 
      examined by subcutaneously injecting SCC25 cells into the area overlaying the 
      calvariae of nude mice. The results demonstrated that the average tumor volume of 
      SCC25 cells with 7ND protein was similar to the average volume of tumors formed 
      by untreated SCC25 cells. Flow cytometric analysis suggested that the CD14(+) 
      subpopulation in the bone marrow of 7ND-treated mice was reduced compared with 
      that of untreated mice. Micro-computed tomography imaging revealed significantly 
      less bone resorption in the calvariae injected with SCC25 cells plus the 7ND 
      protein. Taken together, the results of the present study demonstrated the 
      potential therapeutic value of the 7ND protein. The 7ND MCP-1 variant not only 
      functions in vitro to inhibit osteoclast differentiation, but also reduces the 
      progression of bone invasion by OSCC cells in vivo.
FAU - Luo, Shuyu
AU  - Luo S
AD  - Department of Oral Pathology, School and Hospital of Stomatology, Tianjin Medical 
      University, Heping, Tianjin 300070, P.R. China.
FAU - Zhou, Chuanxiang
AU  - Zhou C
AD  - Department of Oral Pathology, Peking University School and Hospital of 
      Stomatology, Haidian, Beijing 100081, P.R. China.
FAU - Zhang, Jianming
AU  - Zhang J
AD  - Department of Stomatology, General Hospital of Tianjin Medical University, 
      Heping, Tianjin 300052, P.R. China.
FAU - Chen, Mengshan
AU  - Chen M
AD  - School of Public Health, Tianjin Medical University, Heping, Tianjin 300070, P.R. 
      China.
FAU - Li, Hongjie
AU  - Li H
AD  - Department of Oral Pathology, School and Hospital of Stomatology, Tianjin Medical 
      University, Heping, Tianjin 300070, P.R. China.
FAU - Zheng, Shanchuan
AU  - Zheng S
AD  - Department of Stomatology, WuQing People's Hospital, Wuqing, Tianjin 301700, P.R. 
      China.
FAU - Quan, Jingjing
AU  - Quan J
AD  - Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-Sen University 
      and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong 
      510080, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180321
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC5920317
OTO - NOTNLM
OT  - 7ND
OT  - bone invasion
OT  - monocyte chemoattractant protein-1
OT  - oral squamous cell carcinoma
OT  - osteoclasts
EDAT- 2018/05/05 06:00
MHDA- 2018/05/05 06:01
PMCR- 2018/03/21
CRDT- 2018/05/05 06:00
PHST- 2017/03/15 00:00 [received]
PHST- 2018/01/23 00:00 [accepted]
PHST- 2018/05/05 06:00 [entrez]
PHST- 2018/05/05 06:00 [pubmed]
PHST- 2018/05/05 06:01 [medline]
PHST- 2018/03/21 00:00 [pmc-release]
AID - OL-0-0-8308 [pii]
AID - 10.3892/ol.2018.8308 [doi]
PST - ppublish
SO  - Oncol Lett. 2018 May;15(5):7760-7768. doi: 10.3892/ol.2018.8308. Epub 2018 Mar 
      21.