PMID- 2972610
OWN - NLM
STAT- MEDLINE
DCOM- 19881207
LR  - 20190824
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 18
IP  - 4
DP  - 1988 Aug
TI  - Effect of an autoreactive T cell clone from (NZB x NZW)F1 mice on the production 
      of anti-DNA antibodies in vivo and in vitro.
PG  - 281-7
AB  - An autoreactive T lymphocyte clone, designated as F1C4 was established from an 
      autoimmune mouse strain (NZB/NZW)F1. This clone proliferated in the presence of 
      mitomycin C-treated splenic adherent cells (MMC-SAC) from syngeneic mice. This 
      response was dependent on the numbers of MMC-SAC. The specificity of F1C4 for I-A 
      was determined by an inhibition test carried out with monoclonal anti-Ia sera. 
      Furthermore, the F1C4 cells did not exhibit alloreactivity in a proliferation 
      assay and did not react to foreign antigens such as fetal calf serum (FCA) used 
      in the culture medium. When F1C4 cells were cultured with autologous non-T cells 
      in the absence of antigen, they strongly enhanced IgM class anti-ssDNA production 
      from non-T cells of both young and old B/W F1 mice at appropriate cell numbers in 
      vitro. Furthermore, the production of IgG class anti-ssDNA from non-T cells of 
      old B/W F1 mice was also enhanced. The adoptive transfer of F1C4 cells enhanced 
      the levels of both IgM and IgG anti-ssDNA antibodies in the serum of aged B/W F1 
      mice. Moreover, the serum levels of anti-ssDNA of IgG2a and IgG2b subclasses were 
      readily enhanced by the transfer of F1C4 in vivo.
FAU - Naiki, M
AU  - Naiki M
AD  - Institute of Bio-Active Science, Nippon Zoki Pharm. Co., Ltd., Hyogo, Japan.
FAU - Imai, Y
AU  - Imai Y
FAU - Kamita, Y
AU  - Kamita Y
FAU - Osawa, T
AU  - Osawa T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Animals
MH  - Antibodies, Antinuclear/*biosynthesis
MH  - Clone Cells/immunology
MH  - DNA, Single-Stranded/*immunology
MH  - Female
MH  - Immunoglobulin G/biosynthesis
MH  - Mice
MH  - Mice, Inbred NZB
MH  - T-Lymphocytes/*immunology
MH  - T-Lymphocytes, Helper-Inducer/immunology
EDAT- 1988/08/01 00:00
MHDA- 1988/08/01 00:01
CRDT- 1988/08/01 00:00
PHST- 1988/08/01 00:00 [pubmed]
PHST- 1988/08/01 00:01 [medline]
PHST- 1988/08/01 00:00 [entrez]
AID - 0165-2478(88)90175-7 [pii]
AID - 10.1016/0165-2478(88)90175-7 [doi]
PST - ppublish
SO  - Immunol Lett. 1988 Aug;18(4):281-7. doi: 10.1016/0165-2478(88)90175-7.