PMID- 29727832
OWN - NLM
STAT- MEDLINE
DCOM- 20191023
LR  - 20191023
IS  - 1873-3336 (Electronic)
IS  - 0304-3894 (Linking)
VI  - 353
DP  - 2018 Jul 5
TI  - Identification of biphenyl-metabolising microbes in activated biosludge using 
      cultivation-independent and -dependent approaches.
PG  - 534-541
LID - S0304-3894(18)30260-7 [pii]
LID - 10.1016/j.jhazmat.2018.04.028 [doi]
AB  - Microbes have important roles in removing organic pollutants in wastewater 
      treatment plants (WWTPs), especially in mineralising recalcitrant persistent 
      organic pollutants (POPs) such as polychlorinated biphenyls (PCBs). However, the 
      majority of the microorganisms that metabolise these pollutants in situ remain 
      elusive owing to barriers of traditional techniques in unravel 
      yet-to-be-cultivated microbes. In this study, DNA stable-isotope probing (SIP) 
      coupled with high-throughput sequencing was applied to identify the microbes 
      responsible for PCB degradation in the activated biosludge of a WWTP using 
      (13)C-labelled biphenyl (BP). Results of time-course SIP revealed different 
      bacteria and archaea involved in BP metabolism, which dominated the BP-degrading 
      community at different time points. BP degradation by the genera Spartobacteria, 
      Alicyclobacillus, Flavobacterium and the order Cenarchaeales has not been 
      reported previously. The abundance of biphenyl dioxygenase (bphA) genes increased 
      over time and a novel bphA gene was identified from the (13)C-heavy DNA fraction. 
      In addition, three cultivable BP degraders were isolated, but did not participate 
      in BP degradation in situ or contain the identified bphA genes. Taken together, 
      these data reveal the huge potential and important roles of yet-to-be-cultivated 
      microbes responsible for PCB degradation in activated biosludge, providing 
      fundamental knowledge on WWTP management to remove POPs.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Song, Mengke
AU  - Song M
AD  - College of Natural Resources and Environment, South China Agricultural 
      University, Guangzhou, 510642, China; Guangzhou Institute of Geochemistry, 
      Chinese Academy of Sciences, Guangzhou 510640, China.
FAU - Jiang, Longfei
AU  - Jiang L
AD  - Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 
      510640, China.
FAU - Zhang, Dayi
AU  - Zhang D
AD  - School of Environment, Tsinghua University, Beijing 100084, China.
FAU - Luo, Chunling
AU  - Luo C
AD  - College of Natural Resources and Environment, South China Agricultural 
      University, Guangzhou, 510642, China; Guangzhou Institute of Geochemistry, 
      Chinese Academy of Sciences, Guangzhou 510640, China. Electronic address: 
      clluo@gig.ac.cn.
FAU - Yin, Hua
AU  - Yin H
AD  - College of Environment and Energy, South China University of Technology, 
      Guangzhou 510006, China.
FAU - Li, Yongtao
AU  - Li Y
AD  - College of Natural Resources and Environment, South China Agricultural 
      University, Guangzhou, 510642, China.
FAU - Zhang, Gan
AU  - Zhang G
AD  - Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 
      510640, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180416
PL  - Netherlands
TA  - J Hazard Mater
JT  - Journal of hazardous materials
JID - 9422688
RN  - 0 (Carbon Isotopes)
RN  - 0 (DNA Probes)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 0 (Water Pollutants, Chemical)
RN  - 9007-49-2 (DNA)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
RN  - EC 1.13.11.- (Dioxygenases)
SB  - IM
MH  - Archaea/metabolism
MH  - Bacteria/metabolism
MH  - Carbon Isotopes
MH  - DNA/genetics
MH  - DNA Probes
MH  - Dioxygenases/genetics
MH  - High-Throughput Nucleotide Sequencing
MH  - Polychlorinated Biphenyls/*metabolism
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sewage/*microbiology
MH  - Water Pollutants, Chemical/*metabolism
OTO - NOTNLM
OT  - Archaea
OT  - Bacteria
OT  - Biphenyl
OT  - Biphenyl dioxygenase gene
OT  - Stable-isotope probing (SIP)
EDAT- 2018/05/05 06:00
MHDA- 2019/10/24 06:00
CRDT- 2018/05/05 06:00
PHST- 2017/12/09 00:00 [received]
PHST- 2018/04/12 00:00 [revised]
PHST- 2018/04/13 00:00 [accepted]
PHST- 2018/05/05 06:00 [pubmed]
PHST- 2019/10/24 06:00 [medline]
PHST- 2018/05/05 06:00 [entrez]
AID - S0304-3894(18)30260-7 [pii]
AID - 10.1016/j.jhazmat.2018.04.028 [doi]
PST - ppublish
SO  - J Hazard Mater. 2018 Jul 5;353:534-541. doi: 10.1016/j.jhazmat.2018.04.028. Epub 
      2018 Apr 16.