PMID- 2972937
OWN - NLM
STAT- MEDLINE
DCOM- 19881125
LR  - 20180214
IS  - 0028-3835 (Print)
IS  - 0028-3835 (Linking)
VI  - 48
IP  - 3
DP  - 1988 Sep
TI  - Differential effects of pituitary stalk-section on posterior pituitary and 
      hypothalamic contents of prolactin-releasing factor, oxytocin, dopamine and 
      beta-endorphin.
PG  - 314-9
AB  - We have recently shown that the posterior pituitary (neurointermediate lobe) 
      contains a potent prolactin (PRL)-releasing factor (PRF) which is distinct from 
      known PRL secretagogues. Posterior pituitary PRF appears to be a small peptide of 
      an unknown cellular origin. Using pituitary stalk-sectioned (SS) male rats, the 
      objectives of this study were: (1) to determine if PRF is transported from the 
      hypothalamus or is synthesized within the pituitary gland, and (2) to compare 
      changes in PRF activity with alterations in the posterior pituitary content of 
      beta-endorphin (beta-END), oxytocin (OXY), and dopamine (DA). One or two weeks 
      following pituitary SS or sham surgery (SHAM), acid extracts of the posterior 
      pituitary and medial basal hypothalamus (MBH) were analyzed for their hormone 
      content. PRF activity was assessed by determining the stimulation of PRL 
      secretion from perifused anterior pituitary cells, DA was measured by HPLC, and 
      OXY and beta-END levels were determined by RIAs. OXY and DA concentrations in the 
      posterior pituitary were reduced more than 95% at both 1 and 2 weeks after SS. 
      PRF activity in the posterior pituitary was significantly reduced by 75 and 90%, 
      1 and 2 weeks after SS, respectively. In contrast, beta-END levels in the 
      posterior pituitary at these times increased 20 and 60%, as compared to SHAM 
      rats. Unlike the posterior pituitary, OXY levels in the MBH increased 123% 1 week 
      following SS, and 1,260% at 2 weeks. These increases may reflect the accumulation 
      of OXY-containing secretory vesicles in the severed nerves. DA concentrations in 
      the MBH showed a biphasic pattern. DA levels were initially decreased by 70%, and 
      then increased, but remained 30% below SHAM levels. The reason for these 
      alterations in DA levels is not clear.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Hyde, J F
AU  - Hyde JF
AD  - Department of Physiology and Biophysics, Indiana University School of Medicine, 
      Indianapolis.
FAU - Murai, I
AU  - Murai I
FAU - Ben-Jonathan, N
AU  - Ben-Jonathan N
LA  - eng
GR  - DK-08008/DK/NIDDK NIH HHS/United States
GR  - HD-21135/HD/NICHD NIH HHS/United States
GR  - NS-13234/NS/NINDS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Neuroendocrinology
JT  - Neuroendocrinology
JID - 0035665
RN  - 0 (Proteins)
RN  - 50-56-6 (Oxytocin)
RN  - 5Y5F15120W (Thyrotropin-Releasing Hormone)
RN  - 60617-12-1 (beta-Endorphin)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Animals
MH  - Dopamine/*metabolism
MH  - Drinking
MH  - Hypothalamus, Middle/*metabolism
MH  - Male
MH  - Oxytocin/*metabolism
MH  - Pituitary Gland, Posterior/*physiology
MH  - Proteins/metabolism
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Thyrotropin-Releasing Hormone/*metabolism
MH  - beta-Endorphin/*metabolism
EDAT- 1988/09/01 00:00
MHDA- 1988/09/01 00:01
CRDT- 1988/09/01 00:00
PHST- 1988/09/01 00:00 [pubmed]
PHST- 1988/09/01 00:01 [medline]
PHST- 1988/09/01 00:00 [entrez]
AID - 10.1159/000125028 [doi]
PST - ppublish
SO  - Neuroendocrinology. 1988 Sep;48(3):314-9. doi: 10.1159/000125028.