PMID- 29730532
OWN - NLM
STAT- MEDLINE
DCOM- 20180529
LR  - 20180529
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1087-1088
DP  - 2018 Jun 15
TI  - Development and comparison of HPLC-MS/MS and UPLC-MS/MS methods for determining 
      eight coccidiostats in beef.
PG  - 98-107
LID - S1570-0232(17)32084-6 [pii]
LID - 10.1016/j.jchromb.2018.04.044 [doi]
AB  - A high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) 
      method and an ultraperformance liquid chromatography-tandem mass spectrometry 
      (UPLC-MS/MS) method for determining eight coccidiostat (halofuginone, lasalocid, 
      maduramicin, monensin, narasin, nigericin, robenidine and salinomycin) residues 
      in beef were developed and compared. Samples were extracted with a mixture of 
      acetic acid, acetonitrile and ethyl acetate and were then purified on a C(18) 
      solid-phase extraction (SPE) column. The purified samples were analyzed by 
      HPLC-MS/MS and UPLC-MS/MS, using 0.1% formic acid-water solution (A) and pure 
      methanol (B) as the mobile phase. The samples were fractionated on a C(18) column 
      using different gradient elution procedures, followed by qualitative analysis 
      using a mass spectrometer operated in multiple reaction monitoring (MRM) mode 
      with positive electrospray ionization; the external standard method was used for 
      quantitation. At spiked levels that ranged from the limit of quantification (LOQ) 
      to 100 mug/kg, the average recoveries were 71.96%-100.32% and 71.24%-89.24%, with 
      relative standard deviations (RSDs) of 2.65%-12.38% and 2.98%-14.86% for 
      UPLC-MS/MS and HPLC-MS/MS, respectively. The limits of detection (LODs) and LOQs 
      of the eight coccidiostats were 0.14-0.32 mug/kg and 0.43-1.21 mug/kg, 
      respectively, for UPLC-MS/MS analysis and 0.16-0.58 mug/kg and 0.53-1.92 mug/kg, 
      respectively, for HPLC-MS/MS analysis. Both methods had good accuracy and 
      precision, but UPLC-MS/MS had higher sensitivity than HPLC-MS/MS.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Zhao, Xia
AU  - Zhao X
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular 
      Design of Jiangsu Province, Yangzhou 225009, China; Joint International Research 
      Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, China.
FAU - Wang, Bo
AU  - Wang B
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular 
      Design of Jiangsu Province, Yangzhou 225009, China; Joint International Research 
      Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, China.
FAU - Xie, Kaizhou
AU  - Xie K
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular 
      Design of Jiangsu Province, Yangzhou 225009, China; Joint International Research 
      Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, China. 
      Electronic address: yzxkz168@163.com.
FAU - Liu, Jianyu
AU  - Liu J
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular 
      Design of Jiangsu Province, Yangzhou 225009, China; Joint International Research 
      Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, China.
FAU - Zhang, Yangyang
AU  - Zhang Y
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular 
      Design of Jiangsu Province, Yangzhou 225009, China; Joint International Research 
      Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, China.
FAU - Wang, Yajuan
AU  - Wang Y
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular 
      Design of Jiangsu Province, Yangzhou 225009, China; Joint International Research 
      Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, China.
FAU - Guo, Yawen
AU  - Guo Y
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular 
      Design of Jiangsu Province, Yangzhou 225009, China; Joint International Research 
      Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, China.
FAU - Zhang, Genxi
AU  - Zhang G
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular 
      Design of Jiangsu Province, Yangzhou 225009, China; Joint International Research 
      Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, China.
FAU - Dai, Guojun
AU  - Dai G
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular 
      Design of Jiangsu Province, Yangzhou 225009, China; Joint International Research 
      Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, China.
FAU - Wang, Jinyu
AU  - Wang J
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China; Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular 
      Design of Jiangsu Province, Yangzhou 225009, China; Joint International Research 
      Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, China.
LA  - eng
PT  - Journal Article
DEP - 20180430
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Coccidiostats)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Chromatography, High Pressure Liquid/methods
MH  - Coccidiostats/*analysis
MH  - Drug Residues/*analysis
MH  - Limit of Detection
MH  - Linear Models
MH  - Red Meat/*analysis
MH  - Reproducibility of Results
MH  - Tandem Mass Spectrometry/methods
OTO - NOTNLM
OT  - Beef
OT  - Coccidiostats
OT  - HPLC-MS/MS
OT  - UPLC-MS/MS
EDAT- 2018/05/08 06:00
MHDA- 2018/05/31 06:00
CRDT- 2018/05/07 06:00
PHST- 2017/12/06 00:00 [received]
PHST- 2018/04/17 00:00 [revised]
PHST- 2018/04/26 00:00 [accepted]
PHST- 2018/05/08 06:00 [pubmed]
PHST- 2018/05/31 06:00 [medline]
PHST- 2018/05/07 06:00 [entrez]
AID - S1570-0232(17)32084-6 [pii]
AID - 10.1016/j.jchromb.2018.04.044 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Jun 15;1087-1088:98-107. doi: 
      10.1016/j.jchromb.2018.04.044. Epub 2018 Apr 30.