PMID- 29730577
OWN - NLM
STAT- MEDLINE
DCOM- 20181001
LR  - 20181202
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 167
DP  - 2018 Jul 1
TI  - The effects of biomimetically conjugated VEGF on osteogenesis and angiogenesis of 
      MSCs (human and rat) and HUVECs co-culture models.
PG  - 550-559
LID - S0927-7765(18)30273-X [pii]
LID - 10.1016/j.colsurfb.2018.04.060 [doi]
AB  - The purpose of this work was to investigate if the biomimetically conjugated VEGF 
      and HUVECs co-culture could modulate the osteogenic and angiogenic 
      differentiation of MSCs derived from rat and human bone marrow (rMSCs and hMSCs). 
      After treated by ammonia plasma, Poly(lactic-co-glycolic acid) (PLGA) electrospun 
      nanofibers were immobilized with VEGF through heparin to fulfil the sustained 
      release. The proliferation capacity of rMSCs and hMSCs on neat PLGA nanofibers 
      (NF) and VEGF immobilized NF (NF-VEGF) surfaces were assessed by CCK-8 and 
      compared when MSCs were mono-cultured and co-cultured with HUVECs. The effect of 
      VEGF and HUVECs co-culturing on osteogenic and angiogenic differentiation of 
      rMSCs and hMSCs were investigated by calcium deposits and CD31 expression on NF 
      and NF-VEGF surfaces. The results indicated that VEGF has been biomimetically 
      immobilized onto PLGA nanofibers surface and kept sustained release successfully. 
      The CD31 staining results showed that both VEGF and HUVECs co-culture could 
      enhance the angiogenesis of rMSCs and hMSCs. However, the proliferation and 
      osteogenic differentiation of MSCs when cultured with VEGF and HUVECs showed a 
      species dependent response. Taken together, VEGF immobilization and co-culture 
      with HUVECs promoted angiogenesis of MSCs, indicating a good strategy for 
      vascularization in bone tissue engineering.
CI  - Copyright (c) 2018. Published by Elsevier B.V.
FAU - Lu, Lanxin
AU  - Lu L
AD  - Emergency Center of the Affiliated Hospital of Xuzhou Medical University, 
      Institute of Emergency Rescue Medicine, Xuzhou Medical University, Xuzhou, 
      221002, China; Institute for Science and Technology in Medicine, School of 
      Medicine, Keele University, Stoke-on-Trent, ST4 7QB, UK.
FAU - Deegan, Anthony
AU  - Deegan A
AD  - Institute for Science and Technology in Medicine, School of Medicine, Keele 
      University, Stoke-on-Trent, ST4 7QB, UK.
FAU - Musa, Faiza
AU  - Musa F
AD  - Institute for Science and Technology in Medicine, School of Medicine, Keele 
      University, Stoke-on-Trent, ST4 7QB, UK.
FAU - Xu, Tie
AU  - Xu T
AD  - Emergency Center of the Affiliated Hospital of Xuzhou Medical University, 
      Institute of Emergency Rescue Medicine, Xuzhou Medical University, Xuzhou, 
      221002, China. Electronic address: xutie889@163.com.
FAU - Yang, Ying
AU  - Yang Y
AD  - Institute for Science and Technology in Medicine, School of Medicine, Keele 
      University, Stoke-on-Trent, ST4 7QB, UK. Electronic address: y.yang@keele.ac.uk.
LA  - eng
PT  - Journal Article
DEP - 20180430
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Animals
MH  - Biomimetics/methods
MH  - Cell Differentiation/drug effects/physiology
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Human Umbilical Vein Endothelial Cells/drug effects/*physiology
MH  - Humans
MH  - Lactic Acid/chemistry
MH  - Mesenchymal Stem Cells/drug effects/*physiology
MH  - Nanofibers/chemistry
MH  - Neovascularization, Physiologic/drug effects/*physiology
MH  - Osteogenesis/drug effects/*physiology
MH  - Polyglycolic Acid/chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Rats, Sprague-Dawley
MH  - Surface Properties
MH  - Tissue Engineering/methods
MH  - Vascular Endothelial Growth Factor A/*chemistry/pharmacology
OTO - NOTNLM
OT  - Angiogenic
OT  - Co-culture
OT  - HUVECs
OT  - MSCs
OT  - Osteogenic
EDAT- 2018/05/08 06:00
MHDA- 2018/10/03 06:00
CRDT- 2018/05/07 06:00
PHST- 2018/01/23 00:00 [received]
PHST- 2018/04/09 00:00 [revised]
PHST- 2018/04/29 00:00 [accepted]
PHST- 2018/05/08 06:00 [pubmed]
PHST- 2018/10/03 06:00 [medline]
PHST- 2018/05/07 06:00 [entrez]
AID - S0927-7765(18)30273-X [pii]
AID - 10.1016/j.colsurfb.2018.04.060 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2018 Jul 1;167:550-559. doi: 
      10.1016/j.colsurfb.2018.04.060. Epub 2018 Apr 30.