PMID- 29731813 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20200930 IS - 1792-0981 (Print) IS - 1792-1015 (Electronic) IS - 1792-0981 (Linking) VI - 15 IP - 5 DP - 2018 May TI - Sevoflurane-induced memory impairment in the postnatal developing mouse brain. PG - 4097-4104 LID - 10.3892/etm.2018.5950 [doi] AB - The aim of the present study was to confirm that sevoflurane induces memory impairment in the postnatal developing mouse brain and determine its mechanism of action. C57BL/6 mice 7 days old were randomly assigned into a 2.6% sevoflurane (n=68), a 1.3% sevoflurane (n=68) and a control (n=38) group. Blood gas analysis was performed to evaluate hypoxia and respiratory depression during anesthesia in 78 mice. Measurements for expression of caspase-3 by immunohistochemistry, cleavage of poly adenosine diphosphate-ribose polymerase (PARP) by western blotting, as well as levels of brain-derived neurotrophic factor (BDNF), tyrosine kinase receptor type 2 (Ntrk2), pro-BDNF, p75 neurotrophin receptor (p75NTR) and protein kinase B (PKB/Akt) by enzyme-linked immunosorbent assay were performed in the hippocampus of 12 mice from each group. A total of 60 mice underwent the Morris water maze (MWM) test. Results from the MWM test indicated that the time spent in the northwest quadrant and platform site crossovers by mice in the 2.6 and 1.3% sevoflurane groups was significantly lower than that of the control group. Meanwhile, levels of caspase-3 and cleaved PARP in the 2.6 and 1.3% sevoflurane groups were significantly higher than that in the control group. Levels of pro-BDNF and p75NTR were significantly increased and the level of PKB/Akt was significantly decreased following exposure to 2.6% sevoflurane. Finally, the memory of postnatal mice was impaired by sevoflurane, this was determined using a MWM test. Therefore, the results of the current study suggest that caspase-3 induced cleavage of PARP, as well as pro-BDNF, p75NTR and PKB/Akt may be important in sevoflurane-induced memory impairment in the postnatal developing mouse brain. FAU - Lu, Zhijun AU - Lu Z AD - Department of Anesthesia, Rui Jin Hospital Luwan Branch, Shanghai Jiao Tong University School of Medicine, Shanghai 200020, P.R. China. FAU - Sun, Jihui AU - Sun J AD - Department of Anesthesia, Rui Jin Hospital Luwan Branch, Shanghai Jiao Tong University School of Medicine, Shanghai 200020, P.R. China. FAU - Xin, Yichun AU - Xin Y AD - Department of Anesthesia, Rui Jin Hospital Luwan Branch, Shanghai Jiao Tong University School of Medicine, Shanghai 200020, P.R. China. FAU - Chen, Ken AU - Chen K AD - Department of Anesthesia, Rui Jin Hospital Luwan Branch, Shanghai Jiao Tong University School of Medicine, Shanghai 200020, P.R. China. FAU - Ding, Wen AU - Ding W AD - Department of Anesthesia, Rui Jin Hospital Luwan Branch, Shanghai Jiao Tong University School of Medicine, Shanghai 200020, P.R. China. FAU - Wang, Yujia AU - Wang Y AD - Intensive Care Unit, Shanghai Jing'an District Shibei Hospital, Shanghai 200443, P.R. China. LA - eng PT - Journal Article DEP - 20180312 PL - Greece TA - Exp Ther Med JT - Experimental and therapeutic medicine JID - 101531947 PMC - PMC5920718 OTO - NOTNLM OT - hippocampus OT - memory impairment OT - postnatal mouse OT - sevoflurane EDAT- 2018/05/08 06:00 MHDA- 2018/05/08 06:01 PMCR- 2018/03/12 CRDT- 2018/05/08 06:00 PHST- 2016/03/31 00:00 [received] PHST- 2017/03/06 00:00 [accepted] PHST- 2018/05/08 06:00 [entrez] PHST- 2018/05/08 06:00 [pubmed] PHST- 2018/05/08 06:01 [medline] PHST- 2018/03/12 00:00 [pmc-release] AID - ETM-0-0-5950 [pii] AID - 10.3892/etm.2018.5950 [doi] PST - ppublish SO - Exp Ther Med. 2018 May;15(5):4097-4104. doi: 10.3892/etm.2018.5950. Epub 2018 Mar 12.