PMID- 29731855
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 15
IP  - 5
DP  - 2018 May
TI  - Auxiliary diagnostic value of p16 amplification combined with the detection of 
      heterozygous and homozygous loss for urothelial carcinoma.
PG  - 6533-6540
LID - 10.3892/ol.2018.8137 [doi]
AB  - The present study aimed to investigate the significance of detecting cyclin 
      dependent kinase inhibitor 2A (p16) gene aberrations in the diagnosis of 
      urothelial carcinoma (UC) using fluorescence in situ hybridization (FISH). A 
      total of 77 voided urine specimens from 65 patients with UC and 12 patients with 
      benign urinary disease were recruited into the current study. Under a 
      fluorescence microscope, cells with large and irregular nuclei were assessed for 
      chromosomal aberrations. The positive rate of p16 amplification in UC samples was 
      32.3% (21/65), which was significantly higher than that in benign urinary disease 
      samples (16.7%, 2/12; P<0.05). Heterozygous and homozygous loss of p16 was 
      identified in 12 (18.5%) and 23 (35.4%) patients with UC, respectively; p16 
      expression in the remainder of patients was normal. In addition, as tumor stage 
      or grade advanced, the positive rate of p16 aberrations also increased 
      significantly (P<0.05). In conclusion, p16 gene aberrations may serve important 
      roles in the auxiliary diagnosis of UC by FISH and could be utilized to monitor 
      UC progression.
FAU - Mao, Xiaopeng
AU  - Mao X
AD  - Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong 510080, P.R. China.
AD  - Department of Urology Surgery, The First Affiliated Hospital, Sun Yat-sen 
      University, Guangzhou, Guangdong 510080, P.R. China.
FAU - Li, Baimou
AU  - Li B
AD  - Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong 510080, P.R. China.
AD  - Department of Urology Surgery, The First Affiliated Hospital, Sun Yat-sen 
      University, Guangzhou, Guangdong 510080, P.R. China.
FAU - Liang, Ying
AU  - Liang Y
AD  - Department of Nephrology, General Hospital of Guangzhou Military Command of PLA, 
      Guangzhou, Guangdong 510010, P.R. China.
FAU - Li, Shuhua
AU  - Li S
AD  - Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong 510080, P.R. China.
FAU - Zhou, Jianwen
AU  - Zhou J
AD  - Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong 510080, P.R. China.
FAU - He, Qiong
AU  - He Q
AD  - Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong 510080, P.R. China.
FAU - Jiang, Neng
AU  - Jiang N
AD  - Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong 510080, P.R. China.
FAU - Chen, Yangshan
AU  - Chen Y
AD  - Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong 510080, P.R. China.
FAU - Sun, Yu
AU  - Sun Y
AD  - Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong 510080, P.R. China.
FAU - Cui, Yongmei
AU  - Cui Y
AD  - Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong 510080, P.R. China.
FAU - Jiang, Wenting
AU  - Jiang W
AD  - Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong 510080, P.R. China.
FAU - Wang, Han
AU  - Wang H
AD  - Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong 510080, P.R. China.
FAU - Wang, Liantang
AU  - Wang L
AD  - Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong 510080, P.R. China.
FAU - Ke, Zunfu
AU  - Ke Z
AD  - Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, 
      Guangzhou, Guangdong 510080, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180301
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC5920842
OTO - NOTNLM
OT  - amplification
OT  - deletion
OT  - fluorescence in situ hybridization
OT  - p16
OT  - urothelial carcinoma
EDAT- 2018/05/08 06:00
MHDA- 2018/05/08 06:01
PMCR- 2018/03/01
CRDT- 2018/05/08 06:00
PHST- 2017/08/11 00:00 [received]
PHST- 2018/01/10 00:00 [accepted]
PHST- 2018/05/08 06:00 [entrez]
PHST- 2018/05/08 06:00 [pubmed]
PHST- 2018/05/08 06:01 [medline]
PHST- 2018/03/01 00:00 [pmc-release]
AID - OL-0-0-8137 [pii]
AID - 10.3892/ol.2018.8137 [doi]
PST - ppublish
SO  - Oncol Lett. 2018 May;15(5):6533-6540. doi: 10.3892/ol.2018.8137. Epub 2018 Mar 1.