PMID- 29731869
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 15
IP  - 5
DP  - 2018 May
TI  - Effects of human umbilical cord-derived mesenchymal stem cells on hematologic 
      malignancies.
PG  - 6982-6990
LID - 10.3892/ol.2018.8254 [doi]
AB  - Mesenchymal stem cells (MSCs) have been used in hematopoietic stem cell 
      transplantation for years. However, the safety of MSCs applied in various types 
      of hematologic malignancy has not been comprehensively explored. In the present 
      study, the effects of human umbilical cord-derived mesenchymal stem cells 
      (hUC-MSCs) on six representative hematologic malignancy cell lines were explored, 
      including leukemia, multiple myeloma and lymphoma cells. Direct and indirect 
      co-culture models were established, and cell proliferation was assessed by 
      carboxyfluorescein diacetate succinimidyl ester staining. A cytometric bead array 
      cytokine kit was used to quantify cytokines. The expression of interleukin (IL)-6 
      receptor elements on tumor cells was detected by reverse transcription-polymerase 
      chain reaction and flow cytometry, and the effects of exogenous IL-6 on cell 
      proliferation were determined using a Cell Counting kit-8 assay. The results 
      demonstrated that hUC-MSCs inhibited the proliferation of most of the cell lines 
      examined (THP-1, HL-60, K562 and RPMI-8226), but promoted the proliferation of 
      Raji cells. In addition, hUC-MSCs secreted abundant IL-6, promoted the secretion 
      of IL-10 by RPMI-8226 and Raji cells, and inhibited the secretion of tumor 
      necrosis factor-alpha by THP-1 cells. These data indicate a varied effect of hUC-MSCs 
      on various types of hematologic malignancy, including distinct mechanisms of 
      cell-to-cell contact and cytokines. Researchers applying hUC-MSCs in lymphoma 
      should be aware of a potential tumor growth-promoting effect.
FAU - Li, Qian
AU  - Li Q
AD  - Department of Hematopoietic Stem Cell Transplantation, Affiliated Hospital of 
      Academy of Military Medical Sciences, Beijing 100071, P.R. China.
AD  - Cell and Gene Therapy Center, Affiliated Hospital of Academy of Military Medical 
      Sciences, Beijing 100071, P.R. China.
AD  - Department of Oncology, The Army General Hospital, Beijing 100010, P.R. China.
FAU - Pang, Yilin
AU  - Pang Y
AD  - Department of Hematopoietic Stem Cell Transplantation, Affiliated Hospital of 
      Academy of Military Medical Sciences, Beijing 100071, P.R. China.
AD  - Cell and Gene Therapy Center, Affiliated Hospital of Academy of Military Medical 
      Sciences, Beijing 100071, P.R. China.
AD  - Department of Emergency, Beijing Children's Hospital, Capital Medical University, 
      Beijing 100071, P.R. China.
FAU - Liu, Tingting
AU  - Liu T
AD  - Department of Hematopoietic Stem Cell Transplantation, Affiliated Hospital of 
      Academy of Military Medical Sciences, Beijing 100071, P.R. China.
AD  - Cell and Gene Therapy Center, Affiliated Hospital of Academy of Military Medical 
      Sciences, Beijing 100071, P.R. China.
FAU - Tang, Yongyong
AU  - Tang Y
AD  - Department of Hematopoietic Stem Cell Transplantation, Affiliated Hospital of 
      Academy of Military Medical Sciences, Beijing 100071, P.R. China.
AD  - Cell and Gene Therapy Center, Affiliated Hospital of Academy of Military Medical 
      Sciences, Beijing 100071, P.R. China.
FAU - Xie, Jing
AU  - Xie J
AD  - Department of Hematopoietic Stem Cell Transplantation, Affiliated Hospital of 
      Academy of Military Medical Sciences, Beijing 100071, P.R. China.
AD  - Cell and Gene Therapy Center, Affiliated Hospital of Academy of Military Medical 
      Sciences, Beijing 100071, P.R. China.
FAU - Zhang, Bin
AU  - Zhang B
AD  - Department of Hematopoietic Stem Cell Transplantation, Affiliated Hospital of 
      Academy of Military Medical Sciences, Beijing 100071, P.R. China.
AD  - Cell and Gene Therapy Center, Affiliated Hospital of Academy of Military Medical 
      Sciences, Beijing 100071, P.R. China.
FAU - Chen, Hu
AU  - Chen H
AD  - Department of Hematopoietic Stem Cell Transplantation, Affiliated Hospital of 
      Academy of Military Medical Sciences, Beijing 100071, P.R. China.
AD  - Cell and Gene Therapy Center, Affiliated Hospital of Academy of Military Medical 
      Sciences, Beijing 100071, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180313
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC5920879
OTO - NOTNLM
OT  - cytokines
OT  - hematologic malignancy
OT  - interleukin-6
OT  - mesenchymal stem cells
OT  - proliferation
EDAT- 2018/05/08 06:00
MHDA- 2018/05/08 06:01
PMCR- 2018/03/13
CRDT- 2018/05/08 06:00
PHST- 2016/03/03 00:00 [received]
PHST- 2017/07/05 00:00 [accepted]
PHST- 2018/05/08 06:00 [entrez]
PHST- 2018/05/08 06:00 [pubmed]
PHST- 2018/05/08 06:01 [medline]
PHST- 2018/03/13 00:00 [pmc-release]
AID - OL-0-0-8254 [pii]
AID - 10.3892/ol.2018.8254 [doi]
PST - ppublish
SO  - Oncol Lett. 2018 May;15(5):6982-6990. doi: 10.3892/ol.2018.8254. Epub 2018 Mar 
      13.