PMID- 29732148
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2049-9434 (Print)
IS  - 2049-9442 (Electronic)
IS  - 2049-9434 (Linking)
VI  - 8
IP  - 5
DP  - 2018 May
TI  - Effect of molecular hydrogen on uterine inflammation during preterm labour.
PG  - 454-460
LID - 10.3892/br.2018.1082 [doi]
AB  - Intrauterine inflammation causes preterm birth and is associated with 
      complications in preterm neonates. Thus, strategies aimed at suppressing 
      inflammation are expected to be effective for reducing the risk of preterm birth 
      and associated complications. Our previous studies demonstrated that molecular 
      hydrogen (H(2)), an anti-inflammatory agent, prevented inflammation-induced 
      impairment in foetal brain and lung tissues in lipopolysaccharide (LPS)-induced 
      rodent models. However, it remains unclear whether H(2) is capable of inhibiting 
      preterm labour. The aim of the current study was therefore to investigate the 
      effect of H(2) on inflammation-induced preterm labour. Pregnant ICR (CD-1) mice 
      were divided into three groups: Control, LPS and H(2) water (HW) + LPS. In the 
      control and LPS groups, vehicle and LPS, respectively, were intraperitoneally 
      injected on embryonic day 15.5. In the HW + LPS group, HW was administered 24 h 
      prior to LPS injection. The time from LPS administration to parturition was 
      compared between the LPS and HW + LPS groups. Maternal uterus was collected 6 h 
      after LPS injection and the transcript levels of pro-inflammatory cytokines, 
      contractile-associated proteins (CAPs), matrix metalloproteinase-3 (Mmp3) and 
      endothelin-1 (Et1) were assessed by reverse transcription-quantitative polymerase 
      chain reaction. The protein levels of cyclooxygenase-2 (Cox2) were also evaluated 
      by immunohistochemistry. The time from LPS administration to parturition in the 
      HW + LPS group was significantly increased compared with that in the LPS group 
      (33.5+/-3.4 vs. 18.3+/-8.8 h, respectively, P=0.020). H(2) administration also 
      resulted in significantly higher progesterone levels compared with LPS treatment 
      alone (P=0.002). The transcript levels of pro-inflammatory cytokines, CAPs, Mmp3 
      and Et1 in the uteri of the LPS group were significantly higher than those in the 
      control group (all P<0.05). In turn, all these levels with the exception of 
      interleukin-8 and Mmp3 were significantly lower in the HW + LPS group compared 
      with those in the LPS group (all P<0.05). The protein levels of Cox2 in the LPS 
      group were also significantly increased compared with those in the control 
      (P<0.001) and HW + LPS (P=0.003) groups. These results suggest that 
      inflammation-induced changes in the uterus may be ameliorated through maternal 
      H(2) administration. Preventive H(2) administration may therefore represent an 
      effective strategy for the suppression of inflammation during preterm labour.
FAU - Nakano, Tomoko
AU  - Nakano T
AD  - Department of Obstetrics and Gynaecology, Nagoya University Graduate School of 
      Medicine, Nagoya, Aichi 466-8550, Japan.
FAU - Kotani, Tomomi
AU  - Kotani T
AD  - Department of Obstetrics and Gynaecology, Nagoya University Graduate School of 
      Medicine, Nagoya, Aichi 466-8550, Japan.
FAU - Imai, Kenji
AU  - Imai K
AD  - Department of Obstetrics and Gynaecology, Nagoya University Graduate School of 
      Medicine, Nagoya, Aichi 466-8550, Japan.
FAU - Iitani, Yukako
AU  - Iitani Y
AD  - Department of Obstetrics and Gynaecology, Nagoya University Graduate School of 
      Medicine, Nagoya, Aichi 466-8550, Japan.
FAU - Ushida, Takafumi
AU  - Ushida T
AD  - Department of Obstetrics and Gynaecology, Nagoya University Graduate School of 
      Medicine, Nagoya, Aichi 466-8550, Japan.
FAU - Tsuda, Hiroyuki
AU  - Tsuda H
AD  - Department of Obstetrics and Gynaecology, Japanese Red Cross Nagoya Daiichi 
      Hospital, Nagoya, Aichi 453-8511, Japan.
FAU - Li, Hua
AU  - Li H
AD  - Department of Neurology, Yanbian University Hospital, Yanji, Jilin 133000, P.R. 
      China.
FAU - Iwase, Akira
AU  - Iwase A
AD  - Department of Obstetrics and Gynaecology, Nagoya University Graduate School of 
      Medicine, Nagoya, Aichi 466-8550, Japan.
FAU - Toyokuni, Shinya
AU  - Toyokuni S
AD  - Department of Pathology and Biological Responses, Nagoya University Graduate 
      School of Medicine, Nagoya, Aichi 466-8550, Japan.
FAU - Kikkawa, Fumitaka
AU  - Kikkawa F
AD  - Department of Obstetrics and Gynaecology, Nagoya University Graduate School of 
      Medicine, Nagoya, Aichi 466-8550, Japan.
LA  - eng
PT  - Journal Article
DEP - 20180327
PL  - England
TA  - Biomed Rep
JT  - Biomedical reports
JID - 101613227
PMC - PMC5920961
OTO - NOTNLM
OT  - contractile-associated proteins
OT  - inflammation
OT  - preterm labour
OT  - progesterone
EDAT- 2018/05/08 06:00
MHDA- 2018/05/08 06:01
PMCR- 2018/03/27
CRDT- 2018/05/08 06:00
PHST- 2017/08/31 00:00 [received]
PHST- 2018/02/14 00:00 [accepted]
PHST- 2018/05/08 06:00 [entrez]
PHST- 2018/05/08 06:00 [pubmed]
PHST- 2018/05/08 06:01 [medline]
PHST- 2018/03/27 00:00 [pmc-release]
AID - BR-0-0-1082 [pii]
AID - 10.3892/br.2018.1082 [doi]
PST - ppublish
SO  - Biomed Rep. 2018 May;8(5):454-460. doi: 10.3892/br.2018.1082. Epub 2018 Mar 27.