PMID- 29732941
OWN - NLM
STAT- MEDLINE
DCOM- 20191111
LR  - 20191111
IS  - 1557-9042 (Electronic)
IS  - 0897-7151 (Linking)
VI  - 35
IP  - 17
DP  - 2018 Sep 1
TI  - Measurement of Cerebral Biomarkers Proving Traumatic Brain Injuries in 
      Post-Mortem Body Fluids.
PG  - 2044-2055
LID - 10.1089/neu.2017.5441 [doi]
AB  - Until now, it is impossible to identify a fatal traumatic brain injury (TBI) 
      before post-mortem radiological investigations or an autopsy take place. It would 
      be preferable to have an additional diagnostic tool such as post-mortem 
      biochemistry to get greater insight into the pathological pathways and survival 
      times after sustaining TBI. Cerebrospinal fluid (CSF) and serum samples of 84 
      autopsy cases were collected from forensic autopsies with post-mortem intervals 
      (PMI) of up to 148 h. The cases were categorized into a fatal TBI case group 
      (n = 42) and non-TBI controls (n = 42). The values of glial fibrillary acidic 
      protein (GFAP), brain-derived neurotrophic factor (BDNF), and neutrophil 
      gelatinase-associated lipocalin (NGAL) were analyzed by means of quantitative 
      chemiluminescent multiplex immunoassays. The main results indicate that the usage 
      of liquid samples with good macroscopic quality is more relevant for meaningful 
      biomarker analyses than the length of the PMI. All three proteins were shown to 
      differentiate TBI fatalities from the controls in CSF. In serum, only GFAP could 
      be shown to be able to identify TBI cases. This study is the first approach to 
      measure the three proteins together in CSF and serum in autopsy cases. Determined 
      threshold values may differentiate between fatal TBI and control cases. The 
      presented results emphasize the possible use of post-mortem biochemistry as a 
      supplemental tool in everyday forensic routine.
FAU - Ondruschka, Benjamin
AU  - Ondruschka B
AD  - 1 Institute of Legal Medicine, Medical Faculty, University of Leipzig , Leipzig, 
      Germany .
FAU - Sieber, Monique
AU  - Sieber M
AD  - 1 Institute of Legal Medicine, Medical Faculty, University of Leipzig , Leipzig, 
      Germany .
FAU - Kirsten, Holger
AU  - Kirsten H
AD  - 2 Institute for Medical Informatics, Statistics, and Epidemiology, Medical 
      Faculty, University of Leipzig , Leipzig, Germany .
FAU - Franke, Heike
AU  - Franke H
AD  - 3 Rudolf Boehm Institute of Pharmacology and Toxicology, Medical Faculty, 
      University of Leipzig , Leipzig, Germany .
FAU - Dressler, Jan
AU  - Dressler J
AD  - 1 Institute of Legal Medicine, Medical Faculty, University of Leipzig , Leipzig, 
      Germany .
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180706
PL  - United States
TA  - J Neurotrauma
JT  - Journal of neurotrauma
JID - 8811626
RN  - 0 (Biomarkers)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (GFAP protein, human)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (LCN2 protein, human)
RN  - 0 (Lipocalin-2)
RN  - 0 (Nerve Tissue Proteins)
RN  - 7171WSG8A2 (BDNF protein, human)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Autopsy
MH  - Biomarkers/blood/cerebrospinal fluid
MH  - Body Fluids/*chemistry
MH  - *Brain Chemistry
MH  - Brain Injuries, Traumatic/*blood/*cerebrospinal fluid/diagnosis
MH  - Brain-Derived Neurotrophic Factor/blood/cerebrospinal fluid
MH  - Case-Control Studies
MH  - Cause of Death
MH  - Female
MH  - Forensic Pathology
MH  - Glial Fibrillary Acidic Protein/blood/cerebrospinal fluid
MH  - Humans
MH  - Lipocalin-2/blood/cerebrospinal fluid
MH  - Male
MH  - Middle Aged
MH  - Nerve Tissue Proteins/*blood/*cerebrospinal fluid
MH  - Postmortem Changes
MH  - Survival Analysis
MH  - Young Adult
OTO - NOTNLM
OT  - autopsy
OT  - cerebrospinal fluid
OT  - chemiluminescent immunoassay
OT  - fatal traumatic brain injury
OT  - post-mortem biochemistry
EDAT- 2018/05/08 06:00
MHDA- 2019/11/12 06:00
CRDT- 2018/05/08 06:00
PHST- 2018/05/08 06:00 [pubmed]
PHST- 2019/11/12 06:00 [medline]
PHST- 2018/05/08 06:00 [entrez]
AID - 10.1089/neu.2017.5441 [doi]
PST - ppublish
SO  - J Neurotrauma. 2018 Sep 1;35(17):2044-2055. doi: 10.1089/neu.2017.5441. Epub 2018 
      Jul 6.