PMID- 29733303
OWN - NLM
STAT- MEDLINE
DCOM- 20180730
LR  - 20240330
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 134
DP  - 2018 Apr 23
TI  - An In Ovo Model for Testing Insulin-mimetic Compounds.
LID - 10.3791/57237 [doi]
LID - 57237
AB  - Elevated blood glucose levels in type 2 diabetes mellitus (T2DM), a complex and 
      multifactorial metabolic disease, are caused by insulin resistance and beta-cell 
      failure. Various strategies, including the injection of insulin or the usage of 
      insulin-sensitizing drugs, were pursued to treat T2DM or at least reduce the 
      symptoms. In addition, the application of herbal compounds has attracted 
      increasing attention. Thus, it is necessary to find efficient test systems to 
      identify and characterize insulin-mimetic compounds. Here we developed a modified 
      chick embryo model, which enables testing of synthetic compounds and herbal 
      extracts with insulin-mimetic properties. Using a fluorescence microscopy-based 
      primary screen, which quantifies the translocation of Glucose transporter 4 
      (Glut4) to the plasma membrane, we were able to identify compounds, mainly herbal 
      extracts, which lead to an increase of intracellular glucose concentrations in 
      adipocytes. However, the efficacy of these substances requires further 
      verification in a living organism. Thus, we used an in-ovo approach to identify 
      their blood glucose-reducing properties. The approval by an ethics committee is 
      not needed since the use of chicken embryos during the first two-thirds of 
      embryonic development is not considered an animal experiment. Here, the 
      application of this model is described in detail.
FAU - Haselgrubler, Renate
AU  - Haselgrubler R
AD  - University of Applied Sciences Upper Austria.
FAU - Stubl, Flora
AU  - Stubl F
AD  - University of Applied Sciences Upper Austria.
FAU - Stadlbauer, Verena
AU  - Stadlbauer V
AD  - University of Applied Sciences Upper Austria; Austrian Competence Center for Feed 
      and Food Quality, Safety and Innovation.
FAU - Lanzerstorfer, Peter
AU  - Lanzerstorfer P
AD  - University of Applied Sciences Upper Austria.
FAU - Weghuber, Julian
AU  - Weghuber J
AD  - University of Applied Sciences Upper Austria; Austrian Competence Center for Feed 
      and Food Quality, Safety and Innovation; julian.weghuber@fh-wels.at.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Video-Audio Media
DEP - 20180423
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
RN  - 0 (Insulin)
SB  - IM
MH  - Animals
MH  - Chick Embryo
MH  - Chickens
MH  - Diabetes Mellitus, Type 2
MH  - Disease Models, Animal
MH  - Female
MH  - Insulin/*pharmacology
PMC - PMC6100700
EDAT- 2018/05/08 06:00
MHDA- 2018/07/31 06:00
PMCR- 2018/04/23
CRDT- 2018/05/08 06:00
PHST- 2018/05/08 06:00 [entrez]
PHST- 2018/05/08 06:00 [pubmed]
PHST- 2018/07/31 06:00 [medline]
PHST- 2018/04/23 00:00 [pmc-release]
AID - 57237 [pii]
AID - 10.3791/57237 [doi]
PST - epublish
SO  - J Vis Exp. 2018 Apr 23;(134):57237. doi: 10.3791/57237.