PMID- 29733455
OWN - NLM
STAT- MEDLINE
DCOM- 20190508
LR  - 20190508
IS  - 1938-3673 (Electronic)
IS  - 0741-5400 (Linking)
VI  - 104
IP  - 2
DP  - 2018 Aug
TI  - The chemokine fragment CXCL9(74-103) diminishes neutrophil recruitment and joint 
      inflammation in antigen-induced arthritis.
PG  - 413-422
LID - 10.1002/JLB.3MA1217-502R [doi]
AB  - This study investigates if treatment with a peptide corresponding to the 30 
      C-terminal amino acids of CXCL9, CXCL9(74-103), ameliorates joint inflammation in 
      a murine model of antigen-induced arthritis (AIA). AIA was induced in male 
      C57BL/6J mice. Intravenous injection of CXCL9(74-103), simultaneously performed 
      with a tibiofemoral challenge with methylated BSA (mBSA) as antigen in mice 
      immunized with mBSA, diminished the accumulation of leukocytes, in particular 
      neutrophils, in the synovial cavity. The levels of the chemokines CXCL1, CXCL2, 
      and CXCL6 and of the cytokine IL-6 were decreased in inflamed periarticular 
      tissue of mice treated with the CXCL9-derived peptide compared to non-treated AIA 
      mice. In addition, CXCL9(74-103) treatment substantially reduced joint and 
      cartilage damage. CXCL9(74-103) competes with CXCL6 and CCL3 for binding to the 
      glycosaminoglycans heparan sulfate and chondroitin sulfate in vitro. In vivo, 
      CXCL9(74-103) quickly binds to blood vessels in joints as observed by confocal 
      microscopy. Next, we evaluated if later treatment with CXCL9(74-103) had a 
      beneficial impact on joint inflammation. CXCL9(74-103) injection 6 h after mBSA 
      challenge still reduced neutrophil accumulation in the joint, although it did not 
      reduce chemokine and IL-6 concentrations. However, a delay of treatment until 
      12 h after challenge had no effect on cell recruitment and chemokine and IL-6 
      levels. Taken together, we demonstrated that treatment with a peptide, which 
      interferes with the interaction between chemokines and glycosaminoglycans, from 
      the beginning of the disease controlled the massive accumulation of neutrophils 
      in the joint of AIA mice, greatly impacting on joint inflammation and tissue 
      damage.
CI  - (c)2018 Society for Leukocyte Biology.
FAU - Boff, Daiane
AU  - Boff D
AD  - Laboratory of Molecular Immunology, Department of Microbiology and Immunology, 
      Rega Institute, KU Leuven, Leuven, Belgium.
AD  - Departamento de Bioquimica e Imunologia, Instituto de Ciencias Biologicas, 
      Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
FAU - Crijns, Helena
AU  - Crijns H
AD  - Laboratory of Molecular Immunology, Department of Microbiology and Immunology, 
      Rega Institute, KU Leuven, Leuven, Belgium.
FAU - Janssens, Rik
AU  - Janssens R
AD  - Laboratory of Molecular Immunology, Department of Microbiology and Immunology, 
      Rega Institute, KU Leuven, Leuven, Belgium.
FAU - Vanheule, Vincent
AU  - Vanheule V
AD  - Laboratory of Molecular Immunology, Department of Microbiology and Immunology, 
      Rega Institute, KU Leuven, Leuven, Belgium.
FAU - Menezes, Gustavo B
AU  - Menezes GB
AD  - Centro de Biologia Gastrointestinal, Departamento de Morfologia, Universidade 
      Federal de Minas Gerais, Belo Horizonte, Brazil.
FAU - Macari, Soraia
AU  - Macari S
AD  - Departmento de Clinica, Patologia e Cirurgias Odontologicas, Faculdade de 
      Odontologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
FAU - Silva, Tarcilia A
AU  - Silva TA
AD  - Departmento de Clinica, Patologia e Cirurgias Odontologicas, Faculdade de 
      Odontologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
FAU - Amaral, Flavio A
AU  - Amaral FA
AD  - Departamento de Bioquimica e Imunologia, Instituto de Ciencias Biologicas, 
      Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
FAU - Proost, Paul
AU  - Proost P
AD  - Laboratory of Molecular Immunology, Department of Microbiology and Immunology, 
      Rega Institute, KU Leuven, Leuven, Belgium.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180507
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Chemokine CXCL9)
RN  - 0 (Peptides)
SB  - IM
MH  - Animals
MH  - Arthritis, Experimental/*pathology
MH  - Arthritis, Rheumatoid/*pathology
MH  - Chemokine CXCL9/*pharmacology
MH  - Inflammation/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neutrophil Infiltration/*drug effects
MH  - Peptides/pharmacology
OTO - NOTNLM
OT  - chondroitin sulfate
OT  - glycosaminoglycan
OT  - heparan sulfate
OT  - joint damage
OT  - proteoglycan
EDAT- 2018/05/08 06:00
MHDA- 2019/05/09 06:00
CRDT- 2018/05/08 06:00
PHST- 2017/12/15 00:00 [received]
PHST- 2018/03/01 00:00 [revised]
PHST- 2018/04/05 00:00 [accepted]
PHST- 2018/05/08 06:00 [pubmed]
PHST- 2019/05/09 06:00 [medline]
PHST- 2018/05/08 06:00 [entrez]
AID - 10.1002/JLB.3MA1217-502R [doi]
PST - ppublish
SO  - J Leukoc Biol. 2018 Aug;104(2):413-422. doi: 10.1002/JLB.3MA1217-502R. Epub 2018 
      May 7.