PMID- 29733586
OWN - NLM
STAT- MEDLINE
DCOM- 20190917
LR  - 20190917
IS  - 1520-5851 (Electronic)
IS  - 0013-936X (Linking)
VI  - 52
IP  - 15
DP  - 2018 Aug 7
TI  - Biphenyl-Metabolizing Microbial Community and a Functional Operon Revealed in 
      E-Waste-Contaminated Soil.
PG  - 8558-8567
LID - 10.1021/acs.est.7b06647 [doi]
AB  - Primitive electronic waste (e-waste) recycling activities release massive amounts 
      of persistent organic pollutants (POPs) and heavy metals into surrounding soils, 
      posing a major threat to the ecosystem and human health. Microbes capable of 
      metabolizing POPs play important roles in POPs remediation in soils, but their 
      phylotypes and functions remain unclear. Polychlorinated biphenyls (PCBs), one of 
      the main pollutants in e-waste contaminated soils, have drawn increasing 
      attention due to their high persistence, toxicity, and bioaccumulation. In the 
      present study, we employed the culture-independent method of DNA stable-isotope 
      probing to identify active biphenyl and PCB degraders in e-waste-contaminated 
      soil. A total of 19 rare operational taxonomic units and three dominant bacterial 
      genera ( Ralstonia, Cupriavidus, and uncultured bacterium DA101) were enriched in 
      the (13)C heavy DNA fraction, confirming their functions in PCBs metabolism. 
      Additionally, a 13.8 kb bph operon was amplified, containing a bphA gene labeled 
      by (13)C that was concentrated in the heavy DNA fraction. The tetranucleotide 
      signature characteristics of the bph operon suggest that it originated from 
      Ralstonia. The bph operon may be shared by horizontal gene transfer because it 
      contains a transposon gene and is found in various bacterial species. This study 
      gives us a deeper understanding of PCB-degrading mechanisms and provides a 
      potential resource for the bioremediation of PCBs-contaminated soils.
FAU - Jiang, Longfei
AU  - Jiang L
AD  - Guangzhou Institute of Geochemistry, Chinese Academy of Sciences , Guangzhou 
      510640 , China.
FAU - Luo, Chunling
AU  - Luo C
AUID- ORCID: 0000-0003-2359-4246
AD  - Guangzhou Institute of Geochemistry, Chinese Academy of Sciences , Guangzhou 
      510640 , China.
AD  - College of Natural Resources and Environment , South China Agricultural 
      University , Guangzhou 510642 , China.
FAU - Zhang, Dayi
AU  - Zhang D
AUID- ORCID: 0000-0002-4175-5982
AD  - School of Environment , Tsinghua University , Beijing 100084 , China.
FAU - Song, Mengke
AU  - Song M
AD  - College of Natural Resources and Environment , South China Agricultural 
      University , Guangzhou 510642 , China.
FAU - Sun, Yingtao
AU  - Sun Y
AD  - Guangzhou Institute of Geochemistry, Chinese Academy of Sciences , Guangzhou 
      510640 , China.
FAU - Zhang, Gan
AU  - Zhang G
AUID- ORCID: 0000-0002-9010-8140
AD  - Guangzhou Institute of Geochemistry, Chinese Academy of Sciences , Guangzhou 
      510640 , China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180518
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - 0 (Biphenyl Compounds)
RN  - 0 (Soil)
RN  - 0 (Soil Pollutants)
RN  - 2L9GJK6MGN (diphenyl)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
SB  - IM
MH  - Biphenyl Compounds
MH  - *Electronic Waste
MH  - *Microbiota
MH  - Operon
MH  - *Polychlorinated Biphenyls
MH  - Soil
MH  - Soil Microbiology
MH  - *Soil Pollutants
EDAT- 2018/05/08 06:00
MHDA- 2019/09/19 06:00
CRDT- 2018/05/08 06:00
PHST- 2018/05/08 06:00 [pubmed]
PHST- 2019/09/19 06:00 [medline]
PHST- 2018/05/08 06:00 [entrez]
AID - 10.1021/acs.est.7b06647 [doi]
PST - ppublish
SO  - Environ Sci Technol. 2018 Aug 7;52(15):8558-8567. doi: 10.1021/acs.est.7b06647. 
      Epub 2018 May 18.