PMID- 29734056
OWN - NLM
STAT- MEDLINE
DCOM- 20191023
LR  - 20221207
IS  - 1096-0023 (Electronic)
IS  - 1043-4666 (Linking)
VI  - 110
DP  - 2018 Oct
TI  - Promoter variants of TNF-alpha rs1800629 and IL-10 rs1800871 are independently 
      associated with the susceptibility of coronary artery disease in north Indian.
PG  - 131-136
LID - S1043-4666(18)30188-1 [pii]
LID - 10.1016/j.cyto.2018.04.035 [doi]
AB  - BACKGROUND: Tumor necrosis factor-alpha (TNF-alpha) are considered as a pro 
      inflammatory and interleukin-10 (IL-10) anti inflammatory have been shown to 
      predict the risk of incident of coronary artery disease (CAD). The polymorphism 
      at promoter of TNF-alpha and IL-10 has been shown to increase transcriptional 
      activity of the gene and play a important role in patho physiology of CAD. Aim of 
      present study is to examine the impact of the TNF-alpha and IL-10 variant allele on 
      various markers of the CAD and to study its relation with circulating TNF-alpha and 
      IL-10 levels. METHODS: The -308 G/A & -238 G/A of TNF-alpha and -1082 G/A & -819 C/T 
      of IL-10 gene polymorphism has been studied in 301 diagnosed CAD subjects (Age 
      51.50 +/- 9.28; BMI 25.30 +/- 3.58) and 305 healthy controls (Age 51.57 +/- 9.50; BMI 
      24.06 +/- 7.26). These polymorphism of TNF-alpha and IL-10 were detected by real time 
      PCR by using Taqman SNP genotyping assay. Furthermore serum TNF-alpha and IL-10 
      levels were also measured by ELISA. RESULTS: Allelic and genotypic frequencies 
      did not deviate from Hardy-Weinberg equilibrium in the controls (p > 0.05). On 
      allele contrast, significant association with susceptibility to CAD was detected 
      with polymorphisms in TNF-alpha -308 G/A, that variant genotype GA + AA (dominant 
      model) (p = 0.030: OR = 1.61: 95% CI = 1.06-2.44) and variant allele (A) 
      (p = 0.006: OR = 1.71: 95% CI = 1.17-2.51) of TNF-alpha 308 G/A gene was significant 
      highly observed in the cases as compared to control group. Furthermore, variant 
      genotype CT + TT (dominant model) (p = 0.004: OR = 1.62: 95% CI = 1.17-2.24) and 
      variant allele (T) (p < 0.001: OR = 1.49: 95% CI = 1.17-1.89) of IL-10 -819 C/T 
      gene was significant highly observed in the cases as compared to control group. 
      CONCLUSION: Our results suggest that the TNF-alpha G-308A polymorphism independently 
      associated with DBP, cholesterol, triglyceride, LDL, TNF-alpha and IL-10 levels which 
      may be leads to the development of coronary artery disease of North Indians.
CI  - Copyright (c) 2018. Published by Elsevier Ltd.
FAU - Kumari, Reena
AU  - Kumari R
AD  - Department of Biochemistry, KGMU, Lucknow 226003, India; Department of Zoology, 
      University of Lucknow, Lucknow 226007, India. Electronic address: 
      reena.xlnet@gmail.com.
FAU - Kumar, Sandeep
AU  - Kumar S
AD  - Department of Clinical Immunology, SGPGIMS, Lucknow 226014, India.
FAU - Ahmad, Mohammad Kaleem
AU  - Ahmad MK
AD  - Department of Biochemistry, KGMU, Lucknow 226003, India. Electronic address: 
      mohdkaleemahmad@kgmcindia.edu.
FAU - Singh, Rajeev
AU  - Singh R
AD  - Department of Biochemistry, KGMU, Lucknow 226003, India.
FAU - Kant Kumar, Shashi
AU  - Kant Kumar S
AD  - Department of Clinical Immunology, SGPGIMS, Lucknow 226014, India.
FAU - Pradhan, Akshayaya
AU  - Pradhan A
AD  - Department of Cardiology, KGMU, Lucknow 226003, India.
FAU - Chandra, Sharad
AU  - Chandra S
AD  - Department of Cardiology, KGMU, Lucknow 226003, India.
FAU - Kumar, Sudhir
AU  - Kumar S
AD  - Department of Zoology, University of Lucknow, Lucknow 226007, India.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180504
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Biomarkers)
RN  - 0 (IL10 protein, human)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Alleles
MH  - Asian People/*genetics
MH  - Biomarkers
MH  - Case-Control Studies
MH  - Coronary Artery Disease/*genetics
MH  - Female
MH  - Gene Frequency/genetics
MH  - Genetic Predisposition to Disease/*genetics
MH  - Genotype
MH  - Humans
MH  - India
MH  - Interleukin-10/*genetics
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Single Nucleotide/*genetics
MH  - Promoter Regions, Genetic/*genetics
MH  - Tumor Necrosis Factor-alpha/genetics
OTO - NOTNLM
OT  - CAD
OT  - ELISA
OT  - IHD
OT  - Pathophysiology
OT  - Real time PCR
EDAT- 2018/05/08 06:00
MHDA- 2019/10/24 06:00
CRDT- 2018/05/08 06:00
PHST- 2017/11/27 00:00 [received]
PHST- 2018/04/26 00:00 [revised]
PHST- 2018/04/28 00:00 [accepted]
PHST- 2018/05/08 06:00 [pubmed]
PHST- 2019/10/24 06:00 [medline]
PHST- 2018/05/08 06:00 [entrez]
AID - S1043-4666(18)30188-1 [pii]
AID - 10.1016/j.cyto.2018.04.035 [doi]
PST - ppublish
SO  - Cytokine. 2018 Oct;110:131-136. doi: 10.1016/j.cyto.2018.04.035. Epub 2018 May 4.