PMID- 29734171
OWN - NLM
STAT- MEDLINE
DCOM- 20180806
LR  - 20191210
IS  - 1938-2650 (Electronic)
IS  - 0001-5547 (Linking)
VI  - 62
IP  - 4
DP  - 2018
TI  - UroVysion Multi-Target Fluorescence in situ Hybridization Assay for the Detection 
      of Malignant Bile Duct Brushing Specimens: A Comparison with Routine Cytology.
PG  - 295-301
LID - 10.1159/000488636 [doi]
AB  - OBJECTIVE: Routine bile duct brushing cytology is an important diagnostic tool in 
      the evaluation of bile duct stricture. The purpose of this study was to evaluate 
      the performance of the UroVysion fluorescence in situ hybridization (FISH) assay 
      for the detection of malignant bile duct brushing specimens. STUDY DESIGN: 
      Thirty-five bile duct brushing specimens were included in the study. The FISH 
      assay utilized the commercially available UroVysion probes. The indeterminate 
      cytology results were considered as negative for statistical analysis. RESULTS: 
      Twenty-two of 35 patients were diagnosed as having malignancy based on tissue 
      diagnosis or clinical progression of disease by image assessment. The sensitivity 
      of routine cytology and FISH for the detection of malignancy was 14% (3/22) and 
      55% (12/22), respectively (p = 0.003). The specificity of routine cytology and 
      FISH was 100% (13/13) and 62% (8/13), respectively (p = 0.025). The 
      false-positive rate for routine cytology and FISH was 0% (0/13) and 38% (5/13), 
      respectively. CONCLUSIONS: Our study shows that FISH is significantly more 
      sensitive than routine cytology for the detection of malignancy in bile duct 
      brushing specimens. However, in our study, the specificity of FISH was poor 
      compared to the excellent specificity of routine cytology. The compromised 
      specificity of FISH may limit its utility in the detection of malignant bile duct 
      brushing specimens.
CI  - (c) 2018 S. Karger AG, Basel.
FAU - Zhai, Jing
AU  - Zhai J
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
DEP - 20180507
PL  - Switzerland
TA  - Acta Cytol
JT  - Acta cytologica
JID - 0370307
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Bile Duct Neoplasms/complications/*genetics/pathology
MH  - Bile Ducts, Extrahepatic/*pathology
MH  - Biomarkers, Tumor/*genetics
MH  - Child
MH  - Cholangiocarcinoma/complications/*genetics/pathology
MH  - Cholangiopancreatography, Endoscopic Retrograde
MH  - Cholestasis, Extrahepatic/etiology/*genetics/pathology
MH  - *Chromosome Aberrations
MH  - False Positive Reactions
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Liquid Biopsy
MH  - Male
MH  - Middle Aged
MH  - Pancreatic Neoplasms/complications/*genetics/pathology
MH  - Predictive Value of Tests
MH  - Reproducibility of Results
MH  - Young Adult
OTO - NOTNLM
OT  - Bile duct brushing cytology
OT  - Bile duct stricture
OT  - Fluorescence in situ hybridization
EDAT- 2018/05/08 06:00
MHDA- 2018/08/07 06:00
CRDT- 2018/05/08 06:00
PHST- 2017/12/29 00:00 [received]
PHST- 2018/03/21 00:00 [accepted]
PHST- 2018/05/08 06:00 [pubmed]
PHST- 2018/08/07 06:00 [medline]
PHST- 2018/05/08 06:00 [entrez]
AID - 000488636 [pii]
AID - 10.1159/000488636 [doi]
PST - ppublish
SO  - Acta Cytol. 2018;62(4):295-301. doi: 10.1159/000488636. Epub 2018 May 7.