PMID- 29736615 OWN - NLM STAT- MEDLINE DCOM- 20190419 LR - 20211204 IS - 1573-6903 (Electronic) IS - 0364-3190 (Linking) VI - 43 IP - 6 DP - 2018 Jun TI - BDNF Reduces eEF2 Phosphorylation and Enhances Novel Protein Synthesis in the Growth Cones of Dorsal Root Ganglia Neurons. PG - 1242-1249 LID - 10.1007/s11064-018-2541-8 [doi] AB - The local translation, which is regulated by extracellular stimuli such as guidance molecules, in growth cones of neurons provides a molecular mechanism for axonal development. In this study, we performed immunocytochemistry together with atomic force microscopy to investigate the localization of ribosomal proteins in the growth cones of rat dorsal root ganglion (DRG) neurons. The immunoreactivity of ribosomal protein P0/1/2 and S6, and novel protein synthesis were observed in the central, sterically bulky region of growth cones. Brain derived neurotrophic factor (BDNF) reduced the eEF2 phosphorylation, indicating its activation, and enhanced protein synthesis within 30 min. The effects of BDNF were completely inhibited by rapamycin, an inhibitor of mammalian target of rapamycin (mTOR). These results indicated that BDNF rapidly activates translation and enhances novel protein synthesis in growth cones of DRG though the mTOR signaling. FAU - Hoshi, Osamu AU - Hoshi O AD - Department of Anatomy and Physiological Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo, Japan. o-hoshi.aps@tmd.ac.jp. FAU - Sugizaki, Ayana AU - Sugizaki A AD - Department of Anatomy and Physiological Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo, Japan. FAU - Cho, Yuichiro AU - Cho Y AD - Department of Anatomy and Physiological Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo, Japan. FAU - Takei, Nobuyuki AU - Takei N AUID- ORCID: 0000-0002-6122-939X AD - Department of Molecular Neurobiology, Brain Research Institute, Niigata University, Niigata, Japan. nobtak@bri.niigata-u.ac.jp. LA - eng PT - Journal Article DEP - 20180507 PL - United States TA - Neurochem Res JT - Neurochemical research JID - 7613461 RN - 0 (Brain-Derived Neurotrophic Factor) RN - EC 2.7.11.1 (TOR Serine-Threonine Kinases) RN - W36ZG6FT64 (Sirolimus) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/*metabolism MH - Cells, Cultured MH - Ganglia, Spinal/*metabolism MH - Growth Cones/*metabolism MH - Neurogenesis/drug effects MH - Neurons/*metabolism MH - Protein Biosynthesis/drug effects MH - Rats, Wistar MH - Signal Transduction/drug effects MH - Sirolimus/pharmacology MH - TOR Serine-Threonine Kinases/metabolism OTO - NOTNLM OT - AFM OT - BDNF OT - Growth cone OT - Immunocytochemistry OT - Local protein synthesis OT - Local translation OT - SUnSET method OT - eEF2 EDAT- 2018/05/08 06:00 MHDA- 2019/04/20 06:00 CRDT- 2018/05/09 06:00 PHST- 2018/02/25 00:00 [received] PHST- 2018/04/27 00:00 [accepted] PHST- 2018/04/19 00:00 [revised] PHST- 2018/05/08 06:00 [pubmed] PHST- 2019/04/20 06:00 [medline] PHST- 2018/05/09 06:00 [entrez] AID - 10.1007/s11064-018-2541-8 [pii] AID - 10.1007/s11064-018-2541-8 [doi] PST - ppublish SO - Neurochem Res. 2018 Jun;43(6):1242-1249. doi: 10.1007/s11064-018-2541-8. Epub 2018 May 7.