PMID- 29736822 OWN - NLM STAT- MEDLINE DCOM- 20190614 LR - 20190614 IS - 1432-0614 (Electronic) IS - 0175-7598 (Linking) VI - 102 IP - 14 DP - 2018 Jul TI - Immunogenomic screening approach to identify new antigens for the serological diagnosis of chronic Chagas' disease. PG - 6069-6080 LID - 10.1007/s00253-018-8992-7 [doi] AB - Serological tests are preferentially used for the diagnosis of Chagas' disease (CD) during the chronic phase because of the low parasitemia and high anti-Trypanosoma cruzi antibody titers. However, the current methods showed several disadvantages, as contradictory or inconclusive results, mainly related to the characteristics of the antigens used, in general, crude or whole parasites, but also due to antigen production protocol and the experimental conditions used in serological tests. Thus, better-quality serological assays are urgently needed. Here, we performed a wide immunogenomic screen strategy to identify conserved linear B-cell epitopes in the predicted proteome based on genome sequence from T. cruzi strains to will be applied as synthetic peptides in the serodiagnosis of the chronic CD. Three B-cell epitopes derived from mucin-associated surface protein (MASP) family, expressed in both infective parasite stages, trypomastigote and amastigotes, conserved in T. cruzi strains, and highly divergent as compared with Leishmania spp. proteome, were selected for this study. The results demonstrated that synthetic peptide 2 and a mixture of peptides (Mix II: peptides 2 and 3) were able to identify all chronic CD cases, indeterminate or Chagas cardiomyopathy clinical presentation, and simultaneously able to discriminate infections caused by Leishmania parasites, with high accuracy (98.37 and 100.00%, respectively) and agreement (kappa index = 0.967 and 1.000, respectively) with direct methods as compared to current diagnostic pipeline performed by reference laboratories in Brazil. This study represents an interesting strategy for the discovery of new antigens applied to serologic diagnosis of infectious diseases and for the technological development of platforms for large-scale production of diagnostic tests. FAU - Elisei, Rutyanne Maria Tonelli AU - Elisei RMT AD - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. FAU - Matos, Christiane Santos AU - Matos CS AD - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. FAU - Carvalho, Ana Maria Ravena Severino AU - Carvalho AMRS AD - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. FAU - Chaves, Ana Thereza AU - Chaves AT AD - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. FAU - Medeiros, Fernanda Alvarenga Cardoso AU - Medeiros FAC AD - Servico de Doencas Parasitarias, Instituto Octavio Magalhaes, Fundacao Ezequiel Dias (FUNED), Belo Horizonte, Minas Gerais, Brazil. FAU - Barbosa, Ronaldo AU - Barbosa R AD - Servico de Doencas Parasitarias, Instituto Octavio Magalhaes, Fundacao Ezequiel Dias (FUNED), Belo Horizonte, Minas Gerais, Brazil. FAU - Marcelino, Andreza Pain AU - Marcelino AP AD - Servico de Doencas Parasitarias, Instituto Octavio Magalhaes, Fundacao Ezequiel Dias (FUNED), Belo Horizonte, Minas Gerais, Brazil. FAU - Dos Santos Emidio, Kenia AU - Dos Santos Emidio K AD - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. FAU - Coelho, Eduardo Antonio Ferraz AU - Coelho EAF AD - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. AD - Departamento de Patologia Clinica, COLTEC, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. FAU - Duarte, Mariana Costa AU - Duarte MC AD - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. AD - Departamento de Patologia Clinica, COLTEC, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. FAU - de Oliveira Mendes, Tiago Antonio AU - de Oliveira Mendes TA AD - Departamento de Bioquimica e Biologia Molecular, Universidade Federal de Vicosa, Vicosa, Minas Gerais, Brazil. FAU - da Costa Rocha, Manoel Otavio AU - da Costa Rocha MO AD - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. FAU - Menezes-Souza, Daniel AU - Menezes-Souza D AUID- ORCID: 0000-0002-6804-4320 AD - Programa de Pos-Graduacao em Ciencias da Saude: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. dmsouza@ufmg.br. AD - Departamento de Patologia Clinica, COLTEC, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. dmsouza@ufmg.br. LA - eng GR - 23038.004862/2015-74/CAPES/ GR - 87-05/2016/PRPq/UFMG/ GR - 309939/2016-0/CNPq/ PT - Journal Article DEP - 20180507 PL - Germany TA - Appl Microbiol Biotechnol JT - Applied microbiology and biotechnology JID - 8406612 RN - 0 (Antibodies, Protozoan) RN - 0 (Antigens, Protozoan) RN - 0 (Epitopes, B-Lymphocyte) RN - 0 (Proteome) SB - IM MH - Antibodies, Protozoan/blood MH - Antigens, Protozoan/genetics/*immunology MH - Brazil MH - Chagas Disease/*diagnosis/immunology MH - Enzyme-Linked Immunosorbent Assay MH - Epitope Mapping MH - Epitopes, B-Lymphocyte/*immunology MH - *Genomics MH - Humans MH - Proteome MH - Serologic Tests MH - Trypanosoma cruzi/genetics/*immunology OTO - NOTNLM OT - B-cell epitope mapping OT - Chagas' disease OT - Immunodiagnosis OT - Immunoinformatics OT - Peptides OT - Trypanosoma cruzi EDAT- 2018/05/08 06:00 MHDA- 2019/06/15 06:00 CRDT- 2018/05/09 06:00 PHST- 2018/01/05 00:00 [received] PHST- 2018/04/05 00:00 [accepted] PHST- 2018/04/03 00:00 [revised] PHST- 2018/05/08 06:00 [pubmed] PHST- 2019/06/15 06:00 [medline] PHST- 2018/05/09 06:00 [entrez] AID - 10.1007/s00253-018-8992-7 [pii] AID - 10.1007/s00253-018-8992-7 [doi] PST - ppublish SO - Appl Microbiol Biotechnol. 2018 Jul;102(14):6069-6080. doi: 10.1007/s00253-018-8992-7. Epub 2018 May 7.